VEGFA ELISA Kit for Pig(Sus scrofa) | EIAab.comVEGFA ELISA Kit for Pig(Sus scrofa)

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VEGFA (Gene name),

Vascular endothelial growth factor A (Protein name ), VEGFA_PIG from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (1)

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Gene name:

VEGFA(VEGF);

Protein name:

Vascular endothelial growth factor A(VEGF-A);

Alternative:

Vascular permeability factor(VPF);

Organism:

Pig (Sus scrofa).

General Annotation

Sub Unit:

Homodimer; disulfide-linked. Also found as heterodimer with PGF.

Function:

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin.

Subcellular Location:

Secreted Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.

Protein Attributes:

Sequence length:

190

Sequence:

50:

100:

150:

190:

HLFVQDPQTC | KCSCKNTDSR | CKARQLELNE | RTCRCDKPRR |

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF00341

KEGG:

ssc:397157

UniGene:

Ssc.57541

String:

P49151

SMR:

P49151

Uniprot:

P49151

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ELISA Kit for Pig VEGF-A

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E0143h

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ELISA Kit for Pig VEGF-A

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E0143r

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ELISA Kit for Pig VEGF-A

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E0143p

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ELISA Kit for Pig VEGF-A

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E0143c

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ELISA Kit for Pig VEGF-A

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E0143d

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ELISA Kit for Pig VEGF-A

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E0143m

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ELISA Kit for Pig VEGF-A

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E0143b

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CLIA Kit for Pig VEGF-A

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U0143b

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96T

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CLIA Kit for Pig VEGF-A

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U0143d

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CLIA Kit for Pig VEGF-A

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U0143p

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CLIA Kit for Pig VEGF-A

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U0143r

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CLIA Kit for Pig VEGF-A

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U0143h

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96T

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CLIA Kit for Pig VEGF-A

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U0143m

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96T

CLIA Kit for Pig VEGF-A

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U0143c

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Polyclonal Antibody for Pig VEGF-A

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P0143Rb-m

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40ug/0.2ml

Polyclonal Antibody for Pig VEGF-A

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P0143Rb-h

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40ug/0.2ml

Polyclonal Antibody for Pig VEGF-A

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P0143Rb-r

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40ug/0.2ml

Polyclonal Antibody for Pig VEGF-A

Monoclonal Antibody for Pig VEGF-A

Protein for Pig VEGF-A

R&D Technical Data

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 4.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 10.2%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.7%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of VEGF-A into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	86	80-95
Plasma	95	90-102

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of VEGFA into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	103	99-106
Plasma	102	96-107

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGF-A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	84-93%	79-94%	88-96%	78-91%
EDTA plasma(n=5)	78-89%	82-97%	90-102%	89-103%
heparin plasma(n=5)	83-94%	89-101%	87-99%	91-103%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGFA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	98-107%	101-104%	99-106%	100-106%
EDTA plasma(n=5)	100-105%	100-108%	100-105%	99-107%
heparin plasma(n=5)	97-109%	99-106%	95-110%	100-105%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"Development of a Porcine Bladder Acellular Matrix with Well-Preserved Extracellular Bioactive Factors for Tissue Engineering"

Bin Yang, Yifen Zhang, Liuhua Zhou, Zeyu Sun,Junhua Zheng, Yun Chen, and Yutian Dai,

From:TISSUE ENGINEERING

References

1.

"Nucleotide sequence and expression of the porcine vascular endothelial growth factor."
Sharma H.S. , Tang Z.H. , Gho B.C.H. , Verdouw P.D.
Biochim. Biophys. Acta1260:235-238(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Heart.
2.

"PCR cloning of porcine cardiac vascular endothelial growth factor gene."
Lee T. , Canty J.M.
Submitted (2000-11) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]

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