Vascular endothelial growth factor A (Protein name
), VEGFA_RAT from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Related Databases
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Citations (4)
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Gene name:
Vegfa(Vegf);
Protein name:
Vascular endothelial growth factor A(VEGF-A);
Alternative:
Vascular permeability factor(VPF);
Organism:
Rat (Rattus norvegicus).
General Annotation
Sub Unit:
Homodimer; disulfide-linked. Also found as heterodimer with PGF.
Function:
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.
Subcellular Location:
Secreted
VEGF-A120 is acidic and freely secreted. VEGF-A164 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF-A188 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤ 4.8%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤ 10.2%
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤7.5%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤11.7%
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.7%
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Recovery
Recovery was determined by spiking various levels of VEGF-A into serum and plasma .
Sample Type
Average(%)
Recovery Range (%)
Serum
86
80-95
Plasma
95
90-102
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Recovery was determined by spiking various levels of VEGFA into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
103
99-106
Plasma
102
96-107
Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
91-103
Plasma
98
93-106
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGF-A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
84-93%
79-94%
88-96%
78-91%
EDTA plasma(n=5)
78-89%
82-97%
90-102%
89-103%
heparin plasma(n=5)
83-94%
89-101%
87-99%
91-103%
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGFA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
98-107%
101-104%
99-106%
100-106%
EDTA plasma(n=5)
100-105%
100-108%
100-105%
99-107%
heparin plasma(n=5)
97-109%
99-106%
95-110%
100-105%
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
92-105%
90-101%
95-105%
92-109%
EDTA plasma(n=5)
89-95%
93105%
89-94%
90-106%
heparin plasma(n=5)
93-104%
94-110%
101-111%
91-110%
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM VEGF-A164);PROTEIN SEQUENCE OF 27-190
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS VEGF-A188; VEGF-A164; VEGF-A144 AND VEGF-A120)
3.
"Cloning of multiple VEGF splice variants from hypoxic neonatal rat cardiomyocytes." Marion S.
,
Lee T.-C.
Submitted (2001-04) to the EMBL/GenBank/DDBJ databases
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS VEGF-A164 AND VEGF-A188)
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: PROTEIN SEQUENCE OF 27-40;HETERODIMERIZATION WITH PGF
[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for: FUNCTION;TISSUE SPECIFICITY;DEVELOPMENTAL STAGE