VPS4B ELISA Kit for Human(Homo sapiens) | EIAab.comVPS4B ELISA Kit for Human(Homo sapiens)

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VPS4B (Gene name),

Vacuolar protein sorting-associated protein 4B (Protein name ), VPS4B_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

VPS4B(MIG1;SKD1;VPS42);

Protein name:

Vacuolar protein sorting-associated protein 4B;

Alternative:

Suppressor of K(+) transport growth defect 1(Protein SKD1);Cell migration-inducing gene 1 protein;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Proposed to be monomeric or homodimeric in nucleotide-free form and to oligomerize upon binding to ATP to form two stacked hexameric or heptameric rings with a central pore through which ESCRT-III substrates are translocated in an ATP-dependent manner. In vitro, associates on the inside of a helical tubular structure formed by a CHMP2A-CHMP3 polymer. Interacts with CHMP1A, CHMP1B, CHMP2A, CHMP4B and CHMP6. Interacts with VPS4A; the interaction suggests a heteromeric assembly with VPS4A. Interacts with VTA1.

Function:

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).

Subcellular Location:

Prevacuolar compartment membrane Peripheral membrane protein Late endosome membrane Peripheral membrane protein Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells.

Protein Attributes:

Sequence length:

444

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

444:

GDKLLEPVVS | MSDMLRSLSN | TKPTVNEHDL | LKLKKFTEDF | GQEG

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF00004

STRING:

9606.ENSP00000238497

SMR:

O75351

UniGene:

Hs.126550

KEGG:

hsa:9525

MIM:

609983

String:

O75351

Uniprot:

O75351

FOR

ELISA Kit for Human Vacuolar protein sorting-associated protein 4B

CLIA Kit for Human Vacuolar protein sorting-associated protein 4B

Polyclonal Antibody for Human Vacuolar protein sorting-associated protein 4B

Monoclonal Antibody for Human Vacuolar protein sorting-associated protein 4B

Protein for Human Vacuolar protein sorting-associated protein 4B

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Mammalian cells express two VPS4 proteins both of which are involved in intracellular protein trafficking."
Scheuring S. , Roehricht R.A. , Schoening-Burkhardt B. , Beyer A. , Mueller S. , Abts H.F. , Koehrer K.
J. Mol. Biol.312:469-480(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION;INTERACTION WITH VPS4A;SUBCELLULAR LOCATION;TISSUE SPECIFICITY;MUTAGENESIS OF GLU-235
2.

"Comparative sequence and expression analyses of four mammalian VPS4 genes."
Beyer A. , Scheuring S. , Mueller S. , Mincheva A. , Lichter P. , Koehrer K.
Gene305:47-59(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
tissue: Skin.
3.

"Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning."
Mao M. , Fu G. , Wu J.-S. , Zhang Q.-H. , Zhou J. , Kan L.-X. , Huang Q.-H. , He K.-L. , Gu B.-W. , Han Z.-G. , Shen Y. , Gu J. , Yu Y.-P. , Xu S.-H. , Wang Y.-X. , Chen S.-J. , Chen Z.
Proc. Natl. Acad. Sci. U.S.A.95:8175-8180(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Umbilical cord blood.
4.

"Identification of a human cell migration gene 1."
Kim J.W.
Submitted (2003-02) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
5.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Testis.
7.

"CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins."
Howard T.L. , Stauffer D.R. , Degnin C.R. , Hollenberg S.M.
J. Cell Sci.114:2395-2404(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CHMP2A
8.

"The protein network of HIV budding."
von Schwedler U.K. , Stuchell M. , Mueller B. , Ward D.M. , Chung H.-Y. , Morita E. , Wang H.E. , Davis T. , He G.P. , Cimbora D.M. , Scott A. , Kraeusslich H.-G. , Kaplan J. , Morham S.G. , Sundquist W.I.
Cell114:701-713(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN HIV-1 BUDDING;INTERACTION WITH CHMP1A; CHMP1B CHMP2A; CHMP4B AND CHMP6;SUBCELLULAR LOCATION
9.

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
10.

"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S. , Ballif B.A. , Smogorzewska A. , McDonald E.R. III , Hurov K.E. , Luo J. , Bakalarski C.E. , Zhao Z. , Solimini N. , Lerenthal Y. , Shiloh Y. , Gygi S.P. , Elledge S.J.
Science316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Embryonic kidney.
11.

"Two distinct modes of ESCRT-III recognition are required for VPS4 functions in lysosomal protein targeting and HIV-1 budding."
Kieffer C. , Skalicky J.J. , Morita E. , De Domenico I. , Ward D.M. , Kaplan J. , Sundquist W.I.
Dev. Cell15:62-73(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF ALA-15 AND LEU-66
12.

"Phosphoproteome of resting human platelets."
Zahedi R.P. , Lewandrowski U. , Wiesner J. , Wortelkamp S. , Moebius J. , Schuetz C. , Walter U. , Gambaryan S. , Sickmann A.
J. Proteome Res.7:526-534(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Platelet.
13.

"Novel interactions of ESCRT-III with LIP5 and VPS4 and their implications for ESCRT-III disassembly."
Shim S. , Merrill S.A. , Hanson P.I.
Mol. Biol. Cell19:2661-2672(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH VTA1;MUTAGENESIS OF 390-GLY--TRP-396
14.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
15.

"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."
Han G. , Ye M. , Zhou H. , Jiang X. , Feng S. , Jiang X. , Tian R. , Wan D. , Zou H. , Gu J.
Proteomics8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Liver.
16.

"Helical structures of ESCRT-III are disassembled by VPS4."
Lata S. , Schoehn G. , Jain A. , Pires R. , Piehler J. , Goettlinger H.G. , Weissenhorn W.
Science321:1354-1357(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;ASSOCIATION WITH THE CHMP2A-CHMP3 POLYMER;ELECTRON MICROSCOPY
17.

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
18.

"Biochemical analyses of human IST1 and its function in cytokinesis."
Bajorek M. , Morita E. , Skalicky J.J. , Morham S.G. , Babst M. , Sundquist W.I.
Mol. Biol. Cell20:1360-1373(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH IST1
19.

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C. , Kumar C. , Gnad F. , Nielsen M.L. , Rehman M. , Walther T.C. , Olsen J.V. , Mann M.
Science325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
20.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-93; SER-102 AND SER-108;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
21.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
22.

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
23.

"Structural characterization of the MIT domain from human Vps4b."
Takasu H. , Jee J.G. , Ohno A. , Goda N. , Fujiwara K. , Tochio H. , Shirakawa M. , Hiroaki H.
Biochem. Biophys. Res. Commun.334:460-465(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 1-77;DOMAIN;VARIANT MET-58
24.

"Structural and mechanistic studies of VPS4 proteins."
Scott A. , Chung H.Y. , Gonciarz-Swiatek M. , Hill G.C. , Whitby F.G. , Gaspar J. , Holton J.M. , Viswanathan R. , Ghaffarian S. , Hill C.P. , Sundquist W.I.
EMBO J.24:3658-3669(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 123-444;SUBUNIT;INTERACTION WITH VTA1;MUTAGENESIS OF 208-TRP-LEU-209 AND GLY-210
25.

"Solution structure of MIT domain from human SKD1."
RIKEN structural genomics initiative (RSGI)
Submitted (2005-11) to the PDB data bank

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 1-108
26.

"ESCRT-III recognition by VPS4 ATPases."
Stuchell-Brereton M.D. , Skalicky J.J. , Kieffer C. , Karren M.A. , Ghaffarian S. , Sundquist W.I.
Nature449:740-744(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 1-86 IN COMPLEX WITH CHMP2B;INTERACTION WITH CHMP1B

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