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Index > Protein center > Wnk1(Gene name) > Rat
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  • Wnk1 (Gene name),
  • Serine/threonine-protein kinase WNK1 (Protein name ),  WNK1_RAT from NCBI database.
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  • General Annotation
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  • Gene name:
    Wnk1(Hsn2;Prkwnk1);
    Protein name:
    Serine/threonine-protein kinase WNK1;
    Alternative:
    Protein kinase with no lysine 1;Protein kinase lysine-deficient 1;
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    Interacts with SYT2. Interacts with WNK3 and WNK4.
    Function:
    Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L.
    Subcellular Location:
    Cytoplasm
    Protein Attributes:
    Sequence length:
    2126
    Sequence:
    50:
    MSDGTAEKQS | GTPGFLSPPA | PVPKNGSSSD | SSVGEKLGAA | VADSGIGRTE | 
    100:
    EYRRRRHTMD | KDSRGAAATT | TPTEHRFFRR | SVICDSNATA | LELPGLPLSI | 
    150:
    PQPSVPAVVP | QSAPPEPHRE | ETLTATVASQ | VSQQPSAAAS | PGEQAVVGSA | 
    200:
    TATVPSSTSK | DRPVSQPSLV | GSKEEPPPSR | SGSGSGGASA | KEPQEERNQQ | 
    250:
    QDDIEELETK | AVGMSNDGRF | LKFDIEIGRG | SFKTVYKGLD | TETTVEVAWC | 
    300:
    ELQDRKLTKS | ERQRFKEEAE | MLKGLQHPNI | VRFYDSWEST | VKGKKCIVLV | 
    350:
    TELMTSGTLK | TYLKRFKVMK | IKVLRSWCRQ | ILKGLQFLHT | RTPPIIHRDL | 
    400:
    KCDNIFITGP | TGSVKIGDLG | LATLKRASFA | KSVIGTPEFM | APEMYEEKYD | 
    450:
    ESVDVYAFGM | CMLEMATSEY | PYSECQNAAQ | IYRRVTSGVK | PASFDKVAIP | 
    500:
    EVKEIIEGCI | RQNKDERYSI | KDLLNHAFFQ | EETGVRVELA | EEDDGEKIAI | 
    550:
    KLWLRIEDIK | KLKGKYKDNE | AIEFSFDLER | DVPEDVAQEM | VESGYVCEGD | 
    600:
    HKTMAKAIKD | RVSLIKRKRE | QRQLVREEQE | KRKQEESSFK | QQNEQQASVS | 
    650:
    QAGIQPLSVA | STGIPTAPTT | SASVSTQVEP | EEPEADQHQQ | LQYQQPSISV | 
    700:
    LSDGTVDSGQ | GSSVFTESRV | SSQQTVSYGS | QHEQAHSIGT | APGHTVSSIQ | 
    750:
    AQSQPHGVYP | PSSMAQGQNQ | GQPSSSLAGV | LSSQPVQHPQ | QQGIQPTVPP | 
    800:
    QQAVQYSLPQ | AASSSEGTVQ | PVSQPQVSAG | TQSSTQGVSQ | AAPPEQTPIT | 
    850:
    QSQPTQPVPL | VSSVDSAHSD | VASGMSDGNE | NAPSSSGRHE | GRTTKRHYRK | 
    900:
    SVRSRSRHEK | TSRPKLRILN | VSNKGDRVVE | CQLETHNRKM | VTFKFDLDGD | 
    950:
    NPEEIATIMV | NNDFILAIER | ESFVAQVREI | IEKADEMLSE | DVSVEPEGDQ | 
    1000:
    GLESLQGKDD | YGFPGSQKLE | GEFKQPIAVS | SMPQQIGVPT | SSLTQVVHSA | 
    1050:
    GRRFIVSPVP | ESRLRESKIF | TSEIPDPVAA | STSQGPGMNL | SHSASSLSLQ | 
    1100:
    QAFSELKHGQ | MTEGPNTAPP | NFNHPGPTFS | PFLTSIAGVQ | TVAASTPSVS | 
    1150:
    VPITSSPLND | ISTSVMQSEG | ALPTDKGIGG | VTTSTGVVAS | GGLTTLSVSE | 
    1200:
    TPTLSSAVSS | STAPAVVTVS | TTSQPVQAST | SGSIASSTGS | FPSGTFSTTT | 
    1250:
    GTTVSSVAVP | NAKPPTVLLQ | QVAGNTAGVA | IVTSVSTTTP | FPAMASQPSL | 
    1300:
    PLGSSTSAPT | LAETVVVSAH | SLDKASHSST | AGLGLSFCAP | SSSSSSGTAV | 
    1350:
    SSSVSQPGIV | HPLVISSAIA | STPVLPQPAV | PTSTPLLPQV | PNIPPLVQPV | 
    1400:
    ANVPAVQQTL | IHSQPQPALL | PNQPHTHCPE | MDADTQSKAP | GIDDIKTLEE | 
    1450:
    KLRSLFSEHS | SSGTQHASVS | LETPLVVETV | TPGIPTTAVA | PSKLMTSTTS | 
    1500:
    TCLPPTNLPL | GTAGMPVMPV | GTPGQVSTPG | THASAPASTA | TGAKPGTTPP | 
    1550:
    KPSLTKTVVP | PVGTELSAGT | VPCEQLPPFP | GPSLIQTQQP | LEDLDAQLRR | 
    1600:
    TLSPETIPVT | PAVGPLSTMS | STAVTEAGSQ | PQKDGTEVHV | TASSSGAGVV | 
    1650:
    KMGRFQVSVT | MDDAQKERKN | RSEDTKSVHF | ESSTSESSVL | SSSSPESTLV | 
    1700:
    KPEPNGITVS | GISLDVPDST | HRTPTPEAKS | ETGQPTKVGR | FQVTTTANKV | 
    1750:
    GRFSVSRTED | KVTELKKEGP | VTSPFRDSEQ | TVIPAAIPKK | EKPELAEPSH | 
    1800:
    LNGPSSDLEA | AFLSRGGEDG | SGSPHSPPHL | CSKSLPIQTL | SQSLSNSFNS | 
    1850:
    SYMSSDNESD | IEDEDLRLEL | RRLREKHLKE | IQDLQSRQKH | EIESLYTKLG | 
    1900:
    KVPPAVIIPP | AAPLSGRRRR | PTKSKGSKSS | RSSSLGNKSP | QLSGNLSGQS | 
    1950:
    GTSVLNPQQT | LHPPGNTPET | GHNQLLQPLK | PSPSSDNLYS | AFTSDGAISV | 
    2000:
    PSLSAPGQGT | SSTNTVGGTV | SSQAAQAQPP | AMTSSRKGTF | TDDLHKLVDN | 
    2050:
    WARDAMNLSG | RRGSKGHMNY | EGPGMARKFS | APGQLCISMT | SNMGGSTPIS | 
    2100:
    AASATSLGHF | TKSMCPPQQY | GFPAAPFGTQ | WSGTGGPAPQ | PLGQFQPVGT | 
    2126:
    TSLQNFNISN | LQKSISNPPG | SNLRTT
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    String:
    Pfam:
    UniGene:
    Uniprot:
     
    FOR
    ELISA Kit for Rat Serine/threonine-protein kinase WNK1
    ELISA Kit for Rat Serine/threonine-protein kinase WNK1
    ELISA Kit for Rat Serine/threonine-protein kinase WNK1
    ELISA Kit for Rat Serine/threonine-protein kinase WNK1
    CLIA Kit for Rat Serine/threonine-protein kinase WNK1
    CLIA Kit for Rat Serine/threonine-protein kinase WNK1
    CLIA Kit for Rat Serine/threonine-protein kinase WNK1
    CLIA Kit for Rat Serine/threonine-protein kinase WNK1
    Polyclonal Antibody for Rat Serine/threonine-protein kinase WNK1
    Polyclonal Antibody for Rat Serine/threonine-protein kinase WNK1
    Polyclonal Antibody for Rat Serine/threonine-protein kinase WNK1
    Polyclonal Antibody for Rat Serine/threonine-protein kinase WNK1
    Monoclonal Antibody for Rat Serine/threonine-protein kinase WNK1
    Monoclonal Antibody for Rat Serine/threonine-protein kinase WNK1
    Monoclonal Antibody for Rat Serine/threonine-protein kinase WNK1
    Monoclonal Antibody for Rat Serine/threonine-protein kinase WNK1
    Protein for Rat Serine/threonine-protein kinase WNK1
    Protein for Rat Serine/threonine-protein kinase WNK1
    Protein for Rat Serine/threonine-protein kinase WNK1
    Protein for Rat Serine/threonine-protein kinase WNK1

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    Precision
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    Recovery
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    Linearity
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    References
    1. 1.
      "WNK1, a novel mammalian serine/threonine protein kinase lacking the catalytic lysine in subdomain II."
      Xu B.-E. , English J.M. , Wilsbacher J.L. , Stippec S. , Goldsmith E.J. , Cobb M.H.
      J. Biol. Chem.275:16795-16801(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);ENZYME REGULATION;SUBCELLULAR LOCATION;MUTAGENESIS OF LYS-233; CYS-250; LYS-256 AND LYS-259
    2. 2.
      "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
      Gibbs R.A. , Weinstock G.M. , Metzker M.L. , Muzny D.M. , Sodergren E.J. , Scherer S. , Scott G. , Steffen D. , Worley K.C. , Burch P.E. , Okwuonu G. , Hines S. , Lewis L. , Deramo C. , Delgado O. , Dugan-Rocha S. , Miner G. , Morgan M. , more...
      Nature428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 312-2126
      strain: Brown Norway.
    3. 3.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION OF THE HSN2 EXON
    4. 4.
      "Regulation of WNK1 by an autoinhibitory domain and autophosphorylation."
      Xu B.-E. , Min X. , Stippec S. , Lee B.H. , Goldsmith E.J. , Cobb M.H.
      J. Biol. Chem.277:48456-48462(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ENZYME REGULATION;PHOSPHORYLATION AT SER-378 AND SER-382;MUTAGENESIS OF LYS-233; CYS-250; SER-378; SER-382; PHE-524 AND PHE-526
    5. 5.
      "The thiazide-sensitive Na-Cl cotransporter is regulated by a WNK kinase signaling complex."
      Yang C.L. , Zhu X. , Ellison D.H.
      J. Clin. Invest.117:3403-3411(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH WNK3 AND WNK4
    6. 6.
      "Serum and glucocorticoid-induced kinase (SGK) 1 and the epithelial sodium channel are regulated by multiple with no lysine (WNK) family members."
      Heise C.J. , Xu B.E. , Deaton S.L. , Cha S.K. , Cheng C.J. , Earnest S. , Sengupta S. , Juang Y.C. , Stippec S. , Xu Y. , Zhao Y. , Huang C.L. , Cobb M.H.
      J. Biol. Chem.285:25161-25167(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    7. 7.
      "Crystal structure of the kinase domain of WNK1, a kinase that causes a hereditary form of hypertension."
      Min X. , Lee B.-H. , Cobb M.H. , Goldsmith E.J.
      Structure12:1303-1311(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 194-483 OF MUTANT ALA-382;SUBUNIT
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