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Index > Protein center > CHIT1(Gene name) > Human
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  • CHIT1 (Gene name),
  • Chitotriosidase-1 (Protein name ),  CHIT1_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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    • Gene name:
    CHIT1;
    Protein name:
    Chitotriosidase-1;
    Alternative:
    Chitinase-1;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Monomer.
    Function:
    Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity.
    Subcellular Location:
    Secreted Lysosome A small proportion is lysosomal.
    Protein Attributes:
    Sequence length:
    466
    Sequence:
    50:
    MVRSVAWAGF | MVLLMIPWGS | AAKLVCYFTN | WAQYRQGEAR | FLPKDLDPSL | 
    100:
    CTHLIYAFAG | MTNHQLSTTE | WNDETLYQEF | NGLKKMNPKL | KTLLAIGGWN | 
    150:
    FGTQKFTDMV | ATANNRQTFV | NSAIRFLRKY | SFDGLDLDWE | YPGSQGSPAV | 
    200:
    DKERFTTLVQ | DLANAFQQEA | QTSGKERLLL | SAAVPAGQTY | VDAGYEVDKI | 
    250:
    AQNLDFVNLM | AYDFHGSWEK | VTGHNSPLYK | RQEESGAAAS | LNVDAAVQQW | 
    300:
    LQKGTPASKL | ILGMPTYGRS | FTLASSSDTR | VGAPATGSGT | PGPFTKEGGM | 
    350:
    LAYYEVCSWK | GATKQRIQDQ | KVPYIFRDNQ | WVGFDDVESF | KTKVSYLKQK | 
    400:
    GLGGAMVWAL | DLDDFAGFSC | NQGRYPLIQT | LRQELSLPYL | PSGTPELEVP | 
    450:
    KPGQPSEPEH | GPSPGQDTFC | QGKADGLYPN | PRERSSFYSC | AAGRLFQQSC | 
    466:
    PTGLVFSNSC | KCCTWN
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    KEGG:
    hsa:1118
    SMR:
    Q13231
    MIM:
    600031
    STRING:
    9606.ENSP00000255427
    UniGene:
    Hs.201688
    Pfam:
    PF01607
    String:
    Q13231
    Uniprot:
    Q13231
     
    FOR
    ELISA Kit for Human Chitotriosidase-1
    Cat.:
    E13980h
    Price:
    Please sign in first.
    Manual:
    Looking for manual.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Chitotriosidase-1
    Cat.:
    E13980m
    Price:
    Please sign in first.
    Manual:
    Looking for manual.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Chitotriosidase-1
    Cat.:
    U13980m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Chitotriosidase-1
    Cat.:
    U13980h
    Price:
    Please sign in first.
    Manual:
    Looking for manual.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    Polyclonal Antibody for Human Chitotriosidase-1
    Polyclonal Antibody for Human Chitotriosidase-1
    Monoclonal Antibody for Human Chitotriosidase-1
    Monoclonal Antibody for Human Chitotriosidase-1
    Protein for Human Chitotriosidase-1
    Protein for Human Chitotriosidase-1

    R&D Technical Data

    s

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    s
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Precision
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1：2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      "Cloning of a cDNA encoding chitotriosidase, a human chitinase produced by macrophages."
      Boot R.G. , Renkema G.H. , Strijland A. , van Zonneveld A.J. , Aerts J.M.F.G.
      J. Biol. Chem.270:26252-26256(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2);FUNCTION;SUBCELLULAR LOCATION
      tissue: Macrophage.
    2. 2.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4);VARIANT GLY-442
    3. 3.
      "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G. , Barlow K.F. , McLay K.E. , Kaul R. , Swarbreck D. , Dunham A. , Scott C.E. , Howe K.L. , Woodfine K. , Spencer C.C.A. , Jones M.C. , Gillson C. , Searle S. , Zhou Y. , Kokocinski F. , McDonald L. , Evans R. , Phillips K. , more...
      Nature441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    4. 4.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1);VARIANTS SER-102 AND GLY-442
    5. 5.
      "Purification and characterization of human chitotriosidase, a novel member of the chitinase family of proteins."
      Renkema G.H. , Boot R.G. , Muijsers A.O. , Donker-Koopman W.E. , Aerts J.M.F.G.
      J. Biol. Chem.270:2198-2202(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 22-43 AND 178-198;FUNCTION;CATALYTIC ACTIVITY;TISSUE SPECIFICITY
      tissue: Spleen.
    6. 6.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: POLYMORPHISM;FUNCTION (ISOFORM 3)
    7. 7.
      "Structure of human chitotriosidase. Implications for specific inhibitor design and function of mammalian chitinase-like lectins."
      Fusetti F. , von Moeller H. , Houston D. , Rozeboom H.J. , Dijkstra B.W. , Boot R.G. , Aerts J.M.F.G. , van Aalten D.M.F.
      J. Biol. Chem.277:25537-25544(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 22-386 IN COMPLEX WITH CHITOBIOSE AND ALLOSAMIDIN;SUBCELLULAR LOCATION;DISULFIDE BONDS
    8. 8.
      "Crystal structures of allosamidin derivatives in complex with human macrophage chitinase."
      Rao F.V. , Houston D.R. , Boot R.G. , Aerts J.M. , Sakuda S. , van Aalten D.M.
      J. Biol. Chem.278:20110-20116(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 22-385 IN COMPLEX WITH ALLOSAMIDIN
    9. 9.
      "Specificity and affinity of natural product cyclopentapeptide inhibitors against A. fumigatus, human, and bacterial chitinases."
      Rao F.V. , Houston D.R. , Boot R.G. , Aerts J.M. , Hodkinson M. , Adams D.J. , Shiomi K. , Omura S. , van Aalten D.M.
      Chem. Biol.12:65-76(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 22-466 IN COMPLEX WITH THE CYCLOPENTAPEPTIDE INHIBITOR ARGADIN;CATALYTIC ACTIVITY
    10. 10.
      "Type 1 Gaucher disease: null and hypomorphic novel chitotriosidase mutations-implications for diagnosis and therapeutic monitoring."
      Grace M.E. , Balwani M. , Nazarenko I. , Prakash-Cheng A. , Desnick R.J.
      Hum. Mutat.28:866-873(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS LYS-74 AND SER-102
    11. 11.
      "Common G102S polymorphism in chitotriosidase differentially affects activity towards 4-methylumbelliferyl substrates."
      Bussink A.P. , Verhoek M. , Vreede J. , Ghauharali-van der Vlugt K. , Donker-Koopman W.E. , Sprenger R.R. , Hollak C.E. , Aerts J.M. , Boot R.G.
      FEBS J.276:5678-5688(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION OF VARIANT SER-102;SUBCELLULAR LOCATION;CATALYTIC ACTIVITY;GLYCOSYLATION AT ASN-100
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