DHFR ELISA Kit for Human(Homo sapiens) | EIAab.comDHFR ELISA Kit for Human(Homo sapiens)

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Index > Protein center > DHFR(Gene name) > Human

DHFR (Gene name),

Dihydrofolate reductase (Protein name ), DYR_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

DHFR;

Protein name:

Dihydrofolate reductase;

Alternative:

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer.

Function:

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

Subcellular Location:

N/A

Protein Attributes:

Sequence length:

187

Sequence:

50:

100:

150:

187:

EIDLEKYKLL | PEYPGVLSDV | QEEKGIKYKF | EVYEKND

3D Structure:

N/A

Predicted Eptitope:

Please Sign in.

Vaild Sequence:

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Related Databases

String:

P00374

STRING:

9606.ENSP00000396308

UniGene:

Hs.592364

KEGG:

hsa:1719

SMR:

P00374

Pfam:

PF00186

MIM:

126060

Uniprot:

P00374

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ELISA Kit for Human Dihydrofolate reductase

Cat.:

E9276h

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96T

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ELISA Kit for Human Dihydrofolate reductase

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ELISA Kit for Human Dihydrofolate reductase

Cat.:

E9276m

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Looking for manual.

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96T

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ELISA Kit for Human Dihydrofolate reductase

Cat.:

E9276r

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96T

ELISA Kit for Human Dihydrofolate reductase

Cat.:

E9276p

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96T

ELISA Kit for Human Dihydrofolate reductase

Cat.:

E9276b

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96T

CLIA Kit for Human Dihydrofolate reductase

Cat.:

U9276b

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96T

CLIA Kit for Human Dihydrofolate reductase

Cat.:

U9276r

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96T

CLIA Kit for Human Dihydrofolate reductase

Cat.:

U9276p

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Packing:

96T

CLIA Kit for Human Dihydrofolate reductase

MSDS:

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CLIA Kit for Human Dihydrofolate reductase

Cat.:

U9276h

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MSDS:

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Packing:

96T

CLIA Kit for Human Dihydrofolate reductase

Cat.:

U9276m

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96T

Polyclonal Antibody for Human Dihydrofolate reductase

Cat.:

P9276Rb-m

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40ug/0.2ml

Polyclonal Antibody for Human Dihydrofolate reductase

Cat.:

P9276Rb-h

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40ug/0.2ml

Monoclonal Antibody for Human Dihydrofolate reductase

Protein for Human Dihydrofolate reductase

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"The functional human dihydrofolate reductase gene."
Chen M.-J. , Shimada T. , Moulton A.D. , Cline A. , Humphries R.K. , Maizel J. , Nienhuis A.W.
J. Biol. Chem.259:3933-3943(1984) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
2.

"The nucleotide sequence of the cDNA coding for the human dihydrofolic acid reductase."
Masters J.N. , Attardi G.
Gene21:59-63(1983) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
3.

"Human dihydrofolate reductase gene organization. Extensive conservation of the G + C-rich 5' non-coding sequence and strong intron size divergence from homologous mammalian genes."
Yang J.K. , Masters J.N. , Attardi G.
J. Mol. Biol.176:169-187(1984) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
4.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
5.

"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J. , Martin J. , Terry A. , Couronne O. , Grimwood J. , Lowry S. , Gordon L.A. , Scott D. , Xie G. , Huang W. , Hellsten U. , Tran-Gyamfi M. , She X. , Prabhakar S. , Aerts A. , Altherr M. , Bajorek E. , Black S. , more...
Nature431:268-274(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue: Eye.
7.

"Identification and characterization of an inborn error of metabolism caused by dihydrofolate reductase deficiency."
Banka S. , Blom H.J. , Walter J. , Aziz M. , Urquhart J. , Clouthier C.M. , Rice G.I. , de Brouwer A.P. , Hilton E. , Vassallo G. , Will A. , Smith D.E. , Smulders Y.M. , Wevers R.A. , Steinfeld R. , Heales S. , Crow Y.J. , Pelletier J.N. , more...
Am. J. Hum. Genet.88:216-225(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY;VARIANT DHFRD PHE-80
8.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
9.

"The former annotated human pseudogene dihydrofolate reductase-like 1 (DHFRL1) is expressed and functional."
McEntee G. , Minguzzi S. , O'Brien K. , Ben Larbi N. , Loscher C. , O'Fagain C. , Parle-McDermott A.
Proc. Natl. Acad. Sci. U.S.A.108:15157-15162(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CATALYTIC ACTIVITY;RNA-BINDING;BIOPHYSICOCHEMICAL PROPERTIES
10.

"Identification of a de novo thymidylate biosynthesis pathway in mammalian mitochondria."
Anderson D.D. , Quintero C.M. , Stover P.J.
Proc. Natl. Acad. Sci. U.S.A.108:15163-15168(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
11.

"Crystal structure of human dihydrofolate reductase complexed with folate."
Oefner C. , D'Arcy A. , Winkler F.K.
Eur. J. Biochem.174:377-385(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH FOLATE
12.

"Crystal structures of recombinant human dihydrofolate reductase complexed with folate and 5-deazafolate."
Davies J.F. , Delcamp T.J. , Prendergast N.J. , Ashford V.A. , Freisheim J.H. , Kraut J.
Biochemistry29:9467-9479(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEXES WITH FOLATE AND 5-DEAZAFOLATE;SUBUNIT
13.

"Sequence-specific 1H and 15N resonance assignments for human dihydrofolate reductase in solution."
Stockman B.J. , Nirmala N.R. , Wagner G. , Delcamp T.J. , Deyarman M.T. , Freisheim J.H.
Biochemistry31:218-229(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR
14.

"Methotrexate-resistant variants of human dihydrofolate reductase with substitutions of leucine 22. Kinetics, crystallography, and potential as selectable markers."
Lewis W.S. , Cody V. , Galitsky N. , Luft J.R. , Pangborn W. , Chunduru S.K. , Spencer H.T. , Appleman J.R. , Blakley R.L.
J. Biol. Chem.270:5057-5064(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) IN COMPLEXES WITH NADPH; PIRITREXIM AND METOTHREXATE;MUTAGENESIS OF LEU-23
15.

"Comparison of two independent crystal structures of human dihydrofolate reductase ternary complexes reduced with nicotinamide adenine dinucleotide phosphate and the very tight-binding inhibitor PT523."
Cody V. , Galitsky N. , Luft J.R. , Pangborn W. , Blakley R.L. , Gangjee A.
Biochemistry36:13897-13903(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS)
16.

"Structure-based design and synthesis of lipophilic 2,4-diamino-6-substituted quinazolines and their evaluation as inhibitors of dihydrofolate reductases and potential antitumor agents."
Gangjee A. , Vidwans A.P. , Vasudevan A. , Queener S.F. , Kisliuk R.L. , Cody V. , Li R. , Galitsky N. , Luft J.R. , Pangborn W.
J. Med. Chem.41:3426-3434(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEXES WITH NADPH AND QUINAZOLINE INHIBITORS;CATALYTIC ACTIVITY
17.

"Atomic structures of human dihydrofolate reductase complexed with NADPH and two lipophilic antifolates at 1.09 A and 1.05 A resolution."
Klon A.E. , Heroux A. , Ross L.J. , Pathak V. , Johnson C.A. , Piper J.R. , Borhani D.W.
J. Mol. Biol.320:677-693(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.05 ANGSTROMS) IN COMPLEXES WITH NADP AND THE SYNTHETIC INHIBITORS SRI-9439 AND SRI-9662;FUNCTION;CATALYTIC ACTIVITY
18.

"Analysis of two polymorphic forms of a pyrido[2,3-d]pyrimidine N9-C10 reversed-bridge antifolate binary complex with human dihydrofolate reductase."
Cody V. , Galitsky N. , Luft J.R. , Pangborn W. , Gangjee A.
Acta Crystallogr. D59:654-661(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH INHIBITOR
19.

"Analysis of three crystal structure determinations of a 5-methyl-6-N-methylanilino pyridopyrimidine antifolate complex with human dihydrofolate reductase."
Cody V. , Luft J.R. , Pangborn W. , Gangjee A.
Acta Crystallogr. D59:1603-1609(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH INHIBITOR
20.

"Structure determination of tetrahydroquinazoline antifolates in complex with human and Pneumocystis carinii dihydrofolate reductase: correlations between enzyme selectivity and stereochemistry."
Cody V. , Luft J.R. , Pangborn W. , Gangjee A. , Queener S.F.
Acta Crystallogr. D60:646-655(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH NADPH AND INHIBITOR;MUTAGENESIS OF GLN-36 AND ASN-65;CATALYTIC ACTIVITY;BIOPHYSICOCHEMICAL PROPERTIES
21.

"Understanding the role of Leu22 variants in methotrexate resistance: comparison of wild-type and Leu22Arg variant mouse and human dihydrofolate reductase ternary crystal complexes with methotrexate and NADPH."
Cody V. , Luft J.R. , Pangborn W.
Acta Crystallogr. D61:147-155(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEXES WITH NADP AND METHOTREXATE;MUTAGENESIS OF LEU-23
22.

"Solution structure of human dihydrofolate reductase in its complex with trimethoprim and NADPH."
Kovalevskaya N.V. , Smurnyy Y.D. , Polshakov V.I. , Birdsall B. , Bradbury A.F. , Frenkiel T. , Feeney J.
J. Biomol. NMR33:69-72(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR IN COMPLEX WITH TRIMETHOPRIM AND NADPH
23.

"Novel boron-containing, nonclassical antifolates: synthesis and preliminary biological and structural evaluation."
Reynolds R.C. , Campbell S.R. , Fairchild R.G. , Kisliuk R.L. , Micca P.L. , Queener S.F. , Riordan J.M. , Sedwick W.D. , Waud W.R. , Leung A.K. , Dixon R.W. , Suling W.J. , Borhani D.W.
J. Med. Chem.50:3283-3289(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) IN COMPLEXES WITH NADP AND BORON-CONTAINING INHIBITORS;CATALYTIC ACTIVITY
24.

"Correlations of inhibitor kinetics for Pneumocystis jirovecii and human dihydrofolate reductase with structural data for human active site mutant enzyme complexes."
Cody V. , Pace J. , Makin J. , Piraino J. , Queener S.F. , Rosowsky A.
Biochemistry48:1702-1711(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.23 ANGSTROMS) IN COMPLEXES WITH NADP AND THE SYNTHETIC INHIBITOR PY957;CATALYTIC ACTIVITY;BIOPHYSICOCHEMICAL PROPERTIES;MUTAGENESIS OF GLN-36 AND ASN-65
25.

"Multiple conformers in active site of human dihydrofolate reductase F31R/Q35E double mutant suggest structural basis for methotrexate resistance."
Volpato J.P. , Yachnin B.J. , Blanchet J. , Guerrero V. , Poulin L. , Fossati E. , Berghuis A.M. , Pelletier J.N.
J. Biol. Chem.284:20079-20089(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF MUTANT ARG-32/GLU-36 IN COMPLEX WITH METHOTREXATE AND NADP;MUTAGENESIS OF PHE-32 AND GLN-36;CATALYTIC ACTIVITY
26.

"Design, synthesis, and X-ray crystal structure of classical and nonclassical 2-amino-4-oxo-5-substituted-6-ethylthieno[2,3-d]pyrimidines as dual thymidylate synthase and dihydrofolate reductase inhibitors and as potential antitumor agents."
Gangjee A. , Li W. , Kisliuk R.L. , Cody V. , Pace J. , Piraino J. , Makin J.
J. Med. Chem.52:4892-4902(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.24 ANGSTROMS) OF 1-187
27.

"Dihydrofolate reductase deficiency due to a homozygous DHFR mutation causes megaloblastic anemia and cerebral folate deficiency leading to severe neurologic disease."
Cario H. , Smith D.E. , Blom H. , Blau N. , Bode H. , Holzmann K. , Pannicke U. , Hopfner K.P. , Rump E.M. , Ayric Z. , Kohne E. , Debatin K.M. , Smulders Y. , Schwarz K.
Am. J. Hum. Genet.88:226-231(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT DHFRD VAL-153

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