IRAK1 ELISA Kit for Human(Homo sapiens) | EIAab.comIRAK1 ELISA Kit for Human(Homo sapiens)

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IRAK1 (Gene name),

Interleukin-1 receptor-associated kinase 1 (Protein name ), IRAK1_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

IRAK1(IRAK);

Protein name:

Interleukin-1 receptor-associated kinase 1(IRAK-1);

Alternative:

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer (By similarity). Interacts with TOLLIP; this interaction occurs in the cytosol prior to receptor activation. Interacts with MYD88; this interaction recruits IRAK1 to the stimulated receptor complex. Interacts with IL1RL1. Interacts with IRAK1BP1 (By similarity). Associates with TRAF6, PELI1 and IRAK4; this complex recruits TAK1/NR2C2, TAB1 and TAB2 to mediate NF-kappa-B activation. Interacts (when polyubiquitinated) with IKBKG/NEMO.

Function:

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-TAK1/NR2C2-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, TAK1/NR2C2 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.

Subcellular Location:

Cytoplasm Nucleus Translocates to the nucleus when sumoylated.

Protein Attributes:

Sequence length:

712

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

712:

RQGPEESDEF | QS

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

P51617

UniGene:

Hs.522819

String:

P51617

Pfam:

PF00531

STRING:

9606.ENSP00000358997

KEGG:

hsa:3654

MIM:

300283

Uniprot:

P51617

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ELISA Kit for Human IRAK-1

Cat.:

E1514h

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96T

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ELISA Kit for Human IRAK-1

Cat.:

E1514m

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Manual:

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96T

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ELISA Kit for Human IRAK-1

Cat.:

E1514b

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Packing:

96T

CLIA Kit for Human IRAK-1

Cat.:

U1514b

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Packing:

96T

CLIA Kit for Human IRAK-1

Cat.:

U1514h

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Packing:

96T

CLIA Kit for Human IRAK-1

Cat.:

U1514m

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Packing:

96T

Polyclonal Antibody for Human IRAK-1

Cat.:

P1514Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human IRAK-1

Cat.:

P1514Rb-m

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human IRAK-1

Monoclonal Antibody for Human IRAK-1

Protein for Human IRAK-1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.3%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of IRAK1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	105	100-110
Plasma	104	102-112

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IRAK1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	99-110%	102-110%	101-109%	99-109%
EDTA plasma(n=5)	98-113%	99-107%	96-112%	97-108%
heparin plasma(n=5)	100-107%	97-110%	102-107%	100-106%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"IRAK: a kinase associated with the interleukin-1 receptor."
Cao Z. , Henzel W.J. , Gao X.
Science271:1128-1131(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);PARTIAL PROTEIN SEQUENCE;VARIANTS SER-196 AND LEU-532
2.

"Comparative sequence analysis of the MECP2-locus in human and mouse reveals new transcribed regions."
Reichwald K. , Thiesen J. , Wiehe T. , Weitzel J. , Poustka W.A. , Rosenthal A. , Platzer M. , Stratling W.H. , Kioschis P.
Mamm. Genome11:182-190(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1)
3.

"IRAK1b, a novel alternative splice variant of interleukin-1 receptor-associated kinase (IRAK), mediates interleukin-1 signaling and has prolonged stability."
Jensen L.E. , Whitehead A.S.
J. Biol. Chem.276:29037-29044(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2);FUNCTION;TISSUE SPECIFICITY;PHOSPHORYLATION
4.

"A novel splice variant of interleukin-1 receptor (IL-1R)-associated kinase 1 plays a negative regulatory role in Toll/IL-1R-induced inflammatory signaling."
Rao N. , Nguyen S. , Ngo K. , Fung-Leung W.P.
Mol. Cell. Biol.25:6521-6532(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4)
5.

"The DNA sequence of the human X chromosome."
Ross M.T. , Grafham D.V. , Coffey A.J. , Scherer S. , McLay K. , Muzny D. , Platzer M. , Howell G.R. , Burrows C. , Bird C.P. , Frankish A. , Lovell F.L. , Howe K.L. , Ashurst J.L. , Fulton R.S. , Sudbrak R. , Wen G. , Jones M.C. , more...
Nature434:325-337(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
7.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4)
tissue: Placenta.
8.

"MyD88: an adapter that recruits IRAK to the IL-1 receptor complex."
Wesche H. , Henzel W.J. , Shillinglaw W. , Li S. , Cao Z.
Immunity7:837-847(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MYD88
9.

"Tollip, a new component of the IL-1R1 pathway, links IRAK to the IL-1 receptor."
Burns K. , Clatworthy J. , Martin L. , Martinon F. , Plumpton C. , Maschera B. , Lewis A. , Ray K. , Tschopp J. , Volpe F.
Nat. Cell Biol.2:346-351(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TOLLIP
10.

"IRAK4: a novel member of the IRAK family with the properties of an IRAK-kinase."
Li S. , Strelow A. , Fontana E.J. , Wesche H.
Proc. Natl. Acad. Sci. U.S.A.99:5567-5572(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH IRAK4
11.

"Pellino 1 is required for interleukin-1 (IL-1)-mediated signaling through its interaction with the IL-1 receptor-associated kinase 4 (IRAK4)-IRAK-tumor necrosis factor receptor-associated factor 6 (TRAF6) complex."
Jiang Z. , Johnson H.J. , Nie H. , Qin J. , Bird T.A. , Li X.
J. Biol. Chem.278:10952-10956(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PELI1 AND TRAF6
12.

"Characterization of Pellino2, a substrate of IRAK1 and IRAK4."
Strelow A. , Kollewe C. , Wesche H.
FEBS Lett.547:157-161(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF PELI2
13.

"Inhibition of interleukin 1 receptor/Toll-like receptor signaling through the alternatively spliced, short form of MyD88 is due to its failure to recruit IRAK-4."
Burns K. , Janssens S. , Brissoni B. , Olivos N. , Beyaert R. , Tschopp J.
J. Exp. Med.197:263-268(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION BY IRAK4
14.

"Pellino3, a novel member of the Pellino protein family, promotes activation of c-Jun and Elk-1 and may act as a scaffolding protein."
Jensen L.E. , Whitehead A.S.
J. Immunol.171:1500-1506(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PELI3
15.

"Regulation of interleukin receptor-associated kinase (IRAK) phosphorylation and signaling by iota protein kinase C."
Mamidipudi V. , Lin C. , Seibenhener M.L. , Wooten M.W.
J. Biol. Chem.279:4161-4165(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;PHOSPHORYLATION AT THR-66
16.

"Sequential autophosphorylation steps in the interleukin-1 receptor-associated kinase-1 regulate its availability as an adapter in interleukin-1 signaling."
Kollewe C. , Mackensen A.C. , Neumann D. , Knop J. , Cao P. , Li S. , Wesche H. , Martin M.U.
J. Biol. Chem.279:5227-5236(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-209 AND THR-387;DOMAIN;MUTAGENESIS OF THR-209 AND THR-387
17.

"IRAK4 kinase activity is redundant for interleukin-1 (IL-1) receptor-associated kinase phosphorylation and IL-1 responsiveness."
Qin J. , Jiang Z. , Qian Y. , Casanova J.-L. , Li X.
J. Biol. Chem.279:26748-26753(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;MUTAGENESIS OF LYS-239
18.

"IRAK1 serves as a novel regulator essential for lipopolysaccharide-induced interleukin-10 gene expression."
Huang Y. , Li T. , Sane D.C. , Li L.
J. Biol. Chem.279:51697-51703(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF STAT3
19.

"IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST 2 and induces T helper type 2-associated cytokines."
Schmitz J. , Owyang A. , Oldham E. , Song Y. , Murphy E. , McClanahan T.K. , Zurawski G. , Moshrefi M. , Qin J. , Li X. , Gorman D.M. , Bazan J.F. , Kastelein R.A.
Immunity23:479-490(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH IL1RL1
20.

"Interleukin-1 receptor-associated kinase-1 plays an essential role for Toll-like receptor (TLR)7- and TLR9-mediated interferon-{alpha} induction."
Uematsu S. , Sato S. , Yamamoto M. , Hirotani T. , Kato H. , Takeshita F. , Matsuda M. , Coban C. , Ishii K.J. , Kawai T. , Takeuchi O. , Akira S.
J. Exp. Med.201:915-923(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF IRF7
21.

"Smad6 negatively regulates interleukin 1-receptor-Toll-like receptor signaling through direct interaction with the adaptor Pellino-1."
Choi K.C. , Lee Y.S. , Lim S. , Choi H.K. , Lee C.H. , Lee E.K. , Hong S. , Kim I.H. , Kim S.J. , Park S.H.
Nat. Immunol.7:1057-1065(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION IN COMPLEX WITH IRAK4; MYD88; PELI1 AND TRAF6
22.

"Differential regulation of interleukin-1 receptor associated kinase 1 (IRAK1) splice variants."
Su J. , Richter K. , Zhang C. , Gu Q. , Li L.
Mol. Immunol.44:900-905(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUMOYLATION;SUBCELLULAR LOCATION
23.

"The IRAK-catalysed activation of the E3 ligase function of Pellino isoforms induces the Lys63-linked polyubiquitination of IRAK1."
Ordureau A. , Smith H. , Windheim M. , Peggie M. , Carrick E. , Morrice N. , Cohen P.
Biochem. J.409:43-52(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF PELI1
24.

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H. , Olsen J.V. , Bairlein M. , Gnad F. , Oppermann F.S. , Korner R. , Greff Z. , Keri G. , Stemmann O. , Mann M.
Mol. Cell31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
25.

"Lys63-linked polyubiquitination of IRAK-1 is required for interleukin-1 receptor- and toll-like receptor-mediated NF-kappaB activation."
Conze D.B. , Wu C.J. , Thomas J.A. , Landstrom A. , Ashwell J.D.
Mol. Cell. Biol.28:3538-3547(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION AT LYS-134 AND LYS-180 BY TRAF6;INTERACTION WITH IKBKG/NEMO
26.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
27.

"Direct activation of protein kinases by unanchored polyubiquitin chains."
Xia Z.-P. , Sun L. , Chen X. , Pineda G. , Jiang X. , Adhikari A. , Zeng W. , Chen Z.J.
Nature461:114-119(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION
28.

"IRAK1 and IRAK4 promote phosphorylation, ubiquitination, and degradation of MyD88 adaptor-like (Mal)."
Dunne A. , Carpenter S. , Brikos C. , Gray P. , Strelow A. , Wesche H. , Morrice N. , O'Neill L.A.
J. Biol. Chem.285:18276-18282(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF TIRAP
29.

"IRAK1: a critical signaling mediator of innate immunity."
Gottipati S. , Rao N.L. , Fung-Leung W.P.
Cell. Signal.20:269-276(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION
30.

"The Pellino family: IRAK E3 ligases with emerging roles in innate immune signalling."
Moynagh P.N.
Trends Immunol.30:33-42(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION
31.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
32.

"Patterns of somatic mutation in human cancer genomes."
Greenman C. , Stephens P. , Smith R. , Dalgliesh G.L. , Hunter C. , Bignell G. , Davies H. , Teague J. , Butler A. , Stevens C. , Edkins S. , O'Meara S. , Vastrik I. , Schmidt E.E. , Avis T. , Barthorpe S. , Bhamra G. , Buck G. , more...
Nature446:153-158(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS [LARGE SCALE ANALYSIS] MET-398; MET-412; HIS-421; LEU-532; SER-619; MET-625; TRP-638 AND GLY-690

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