LILRB2 ELISA Kit for Human(Homo sapiens) | EIAab.comLILRB2 ELISA Kit for Human(Homo sapiens)

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LILRB2 (Gene name),

Leukocyte immunoglobulin-like receptor subfamily B member 2 (Protein name ), LIRB2_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

LILRB2(ILT4;LIR2;MIR10);

Protein name:

Leukocyte immunoglobulin-like receptor subfamily B member 2(LIR-2;Leukocyte immunoglobulin-like receptor 2);

Alternative:

Immunoglobulin-like transcript 4(ILT-4);CD85 antigen-like family member D;Monocyte/macrophage immunoglobulin-like receptor 10(MIR-10);

CD:

CD85d;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Binds PTPN6 when phosphorylated. Binds FCGR1A.

Function:

Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance. Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

Subcellular Location:

Membrane Single-pass type I membrane protein

Protein Attributes:

Sequence length:

598

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

598:

AAASEAPQDV | TYAQLHSLTL | RRKATEPPPS | QEREPPAEPS | IYATLAIH

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

SMR:

Q8N423

UniGene:

Hs.655652

KEGG:

hsa:10288

MIM:

604815

STRING:

9606.ENSP00000375629

String:

Q8N423

Uniprot:

Q8N423

FOR

ELISA Kit for Human LIR-2

Cat.:

E7745h

Price:

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Manual:

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MSDS:

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Packing:

96T

Range:

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CLIA Kit for Human LIR-2

Cat.:

U7745h

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Manual:

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MSDS:

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Packing:

96T

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Polyclonal Antibody for Human LIR-2

Cat.:

P7745Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human LIR-2

Protein for Human LIR-2

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤12.7%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LIRB2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-107%	95-104%	93-103%
EDTA plasma(n=5)	89-107%	93-105%	90-104%	92-106%
heparin plasma(n=5)	93-104%	94-101%	91-106%	94-100%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"A common inhibitory receptor for major histocompatibility complex class I molecules on human lymphoid and myelomonocytic cells."
Colonna M. , Navarro F. , Bellon T. , Llano M. , Garcia P. , Samaridis J. , Angman L. , Cella M. , Lopez-Botet M.
J. Exp. Med.186:1809-1818(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3);VARIANTS ASP-161; TYR-300; TRP-306 AND HIS-322
2.

"A family of human lymphoid and myeloid Ig-like receptors, some of which bind to MHC class I molecules."
Borges L. , Hsu M.-L. , Fanger N. , Kubin M. , Cosman D.
J. Immunol.159:5192-5196(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);TISSUE SPECIFICITY;FUNCTION;VARIANTS ASP-161; TYR-300; TRP-306 AND HIS-322
tissue: Dendritic cell.
tissue: Peripheral blood monocyte.
3.

Canavez F.C.
Submitted (2000-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2);VARIANTS ASP-161; TYR-300; TRP-306 AND HIS-322
4.

"The DNA sequence and biology of human chromosome 19."
Grimwood J. , Gordon L.A. , Olsen A.S. , Terry A. , Schmutz J. , Lamerdin J.E. , Hellsten U. , Goodstein D. , Couronne O. , Tran-Gyamfi M. , Aerts A. , Altherr M. , Ashworth L. , Bajorek E. , Black S. , Branscomb E. , Caenepeel S. , Carrano A.V. , more...
Nature428:529-535(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1);VARIANTS ASP-161; TYR-300; TRP-306 AND HIS-322
tissue: Peripheral blood leukocyte.
6.

"The MHC class I binding proteins LIR-1 and LIR-2 inhibit Fc receptor-mediated signaling in monocytes."
Fanger N.A. , Cosman D. , Peterson L. , Braddy S.C. , Maliszewski C.R. , Borges L.
Eur. J. Immunol.28:3423-3434(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PTPN6 AND FCGR1A;PHOSPHORYLATION;TISSUE SPECIFICITY;FUNCTION
7.

"Tolerization of dendritic cells by T(S) cells: the crucial role of inhibitory receptors ILT3 and ILT4."
Chang C.C. , Ciubotariu R. , Manavalan J.S. , Yuan J. , Colovai A.I. , Piazza F. , Lederman S. , Colonna M. , Cortesini R. , Dalla-Favera R. , Suciu-Foca N.
Nat. Immunol.3:237-243(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
8.

"Human inhibitory receptors Ig-like transcript 2 (ILT2) and ILT4 compete with CD8 for MHC class I binding and bind preferentially to HLA-G."
Shiroishi M. , Tsumoto K. , Amano K. , Shirakihara Y. , Colonna M. , Braud V.M. , Allan D.S.J. , Makadzange A. , Rowland-Jones S. , Willcox B.E. , Jones E.Y. , van der Merwe P.A. , Kumagai I. , Maenaka K.
Proc. Natl. Acad. Sci. U.S.A.100:8856-8861(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TYPE I MHC ALLELES;FUNCTION
9.

"Crystal structure of LIR-2 (ILT4) at 1.8 A: differences from LIR-1 (ILT2) in regions implicated in the binding of the human cytomegalovirus class I MHC homolog UL18."
Willcox B.E. , Thomas L.M. , Chapman T.L. , Heikema A.P. , West A.P. Jr. , Bjorkman P.J.
BMC Struct. Biol.2:6-6(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 22-219

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