NF-kappa-B essential modulator (Protein name
), NEMO_HUMAN from NCBI database.
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General Annotation
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Antigen Annotation
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Predicted Eptitope
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Gene name:
IKBKG(FIP3;NEMO);
Protein name:
NF-kappa-B essential modulator(NEMO);
Alternative:
IkB kinase-associated protein 1(IKKAP1);FIP-3;NF-kappa-B essential modifier;Inhibitor of nuclear factor kappa-B kinase subunit gamma(I-kappa-B kinase subunit gamma;IKK-gamma;IKKG;IkB kinase subunit gamma);
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
Homodimer; disulfide-linked. Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. The IKK complex associates with TERF2IP/RAP1, leading to promote IKK-mediated phosphorylation of RELA/p65. Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Interacts with COPS3, CYLD, NALP2, TRPC4AP and LRDD. Interacts with ATM; the complex is exported from the nucleus. Interacts with TRAF6. Interacts with HTLV-1 Tax oncoprotein; the interaction activates IKBKG. Interacts with TANK; the interaction is enhanced by IKBKE and TBK1. Part of a ternary complex consisting of TANK, IKBKB and IKBKG. Interacts with ZFAND5. Interacts with RIPK2.
Function:
Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity (By similarity). Essential for viral activation of IRF3.
Subcellular Location:
Cytoplasm
Nucleus
Sumoylated NEMO accumulates in the nucleus in response to genotoxic stress.
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.8%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤9.2%
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Recovery
Recovery was determined by spiking various levels of NEMOinto serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
96
89-102
Plasma
97
91-105
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NEMO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
90-103%
92-102%
89-104%
91-107%
EDTA plasma(n=5)
93-101%
94-100%
90-103%
89-106%
heparin plasma(n=5)
92-103%
91-105%
94-101%
93-102%
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Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);PARTIAL PROTEIN SEQUENCE
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT IP VAL-407
"Ikbkg gene modulates the herpes virus susceptibility in mice." Perelygin A.A.
,
Perelygina L.M.
Submitted (2002-05) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)
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Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3)
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Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
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Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
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Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 51-419 (ISOFORM 1);PROTEIN SEQUENCE OF 144-159
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Cited for: SUBUNIT OF A COMPLEX CONTAINING CREBBP; NCOA2; NCOA3; IKKA AND IKKB
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Cited for: SUMOYLATION AT LYS-277 AND LYS-309;UBIQUITINATION AT LYS-277 AND LYS-309;MUTAGENESIS OF LYS-277 AND LYS-309;SUBCELLULAR LOCATION;CHARACTERIZATION OF VARIANT EDAID ARG-417
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Cited for: SELF-ASSOCIATION;COMPOSITION OF THE IKK COMPLEX
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Cited for: UBIQUITINATION AT LYS-285;MUTAGENESIS OF LYS-115; LYS-224; LYS-285 AND LYS-399
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Cited for: FUNCTION;UBIQUITINATION AT LYS-399;MUTAGENESIS OF LYS-399
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Cited for: UBIQUITIN-BINDING;MUTAGENESIS OF LEU-329;CHARACTERIZATION OF VARIANT EDAID ASN-311
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Cited for: INTERACTION WITH ATM;PHOSPHORYLATION AT SER-85;MUTAGENESIS OF SER-85
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Cited for: INTERACTION WITH IKBKB;PHOSPHORYLATION AT SER-68;MUTAGENESIS OF SER-68
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Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
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Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
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Cited for: UBIQUITIN-BINDING;MUTAGENESIS OF VAL-414 AND MET-415;CHARACTERIZATION OF VARIANT IP VAL-407
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Cited for: FUNCTION;DOMAIN LEUCINE-ZIPPER AND C2HC-TYPE ZINC-FINGER
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Cited for: MUTAGENESIS OF GLU-296; PHE-312; GLU-315; ALA-323; LEU-329 AND LEU-336
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Cited for: INTERACTION WITH SHIGELLA FLEXNERI IPAH9.8;UBIQUITINATION AT LYS-309 AND LYS-321
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Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
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Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
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Cited for: UBIQUITIN-BINDING;CHARACTERIZATION OF VARIANT EDAID ASN-311
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Cited for: UBIQUITINATION AT LYS-277; LYS-285; LYS-309; LYS-326; LYS-111; LYS-143; LYS-226; LYS-246; LYS-264; LYS-292 AND LYS-302 BY THE LUBAC COMPLEX;UBIQUITINATION AT LYS-139 AND LYS-283
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Cited for: STRUCTURE BY NMR OF 394-419 OF WILD-TYPE AND MUTANT PHE-417;DOMAIN ZINC-FINGER
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Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 44-111 IN COMPLEX WITH CHUK AND IKBKB;SUBUNIT
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Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 246-337;UBIQUITIN-BINDING;MUTAGENESIS OF VAL-300; LEU-301; GLN-304; ILE-307; TYR-308; PHE-312; GLN-313 AND GLN-317;CHARACTERIZATION OF VARIANT EDAID ASN-311;CHARACTERIZATION OF VARIANT IMD33 ALA-315;CHARACTERIZATION OF VARIANTS GLN-319 AND IP PRO-323
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Cited for: VARIANTS EDAID PRO-175; PRO-227; GLY-288; ASN-311; ARG-417 AND PHE-417
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Cited for: VARIANTS IP LYS-57; LYS-90 DEL AND TRP-123;VARIANT ASN-113;CHARACTERIZATION OF VARIANTS IP LYS-57; LYS-90 DEL AND TRP-123;CHARACTERIZATION OF VARIANT ASN-113
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Cited for: VARIANTS EDAID ARG-153 AND ARG-417;VARIANT NEMOID TYR-417
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Cited for: VARIANT IP PRO-323;CHARACTERIZATION OF VARIANT IP PRO-323;INTERACTION WITH TRAF6