PIK3CA ELISA Kit for Human(Homo sapiens) | EIAab.comPIK3CA ELISA Kit for Human(Homo sapiens)

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PIK3CA (Gene name),

Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (Protein name ), PK3CA_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

PIK3CA;

Protein name:

Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform(PI3-kinase subunit alpha;PI3K-alpha;PI3Kalpha;PtdIns-3-kinase subunit alpha);

Alternative:

Phosphoinositide-3-kinase catalytic alpha polypeptide;Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha(PtdIns-3-kinase subunit p110-alpha;p110alpha);2.7.11.1;Serine/threonine protein kinase PIK3CA);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Heterodimer of a catalytic subunit PIK3CA and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interacts with IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity). Interacts with RASD2 (By similarity). Interacts with APPL1. Interacts with HRAS1 and KRAS (By similarity). Interaction with HRAS1/KRAS is required for PI3K pathway signaling and cell proliferation stimulated by EGF and FGF2.

Function:

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol-3,4,5-triphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS.

Subcellular Location:

N/A

Protein Attributes:

Sequence length:

1068

Sequence:

50:

100:

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650:

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750:

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850:

900:

950:

1000:

1050:

1068:

WTTKMDWIFH | TIKQHALN

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

STRING:

9606.ENSP00000263967

UniGene:

Hs.553498

Pfam:

PF00454

MIM:

114480

SMR:

P42336

KEGG:

hsa:5290

String:

P42336

Uniprot:

P42336

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ELISA Kit for Human PI3-kinase subunit alpha

Cat.:

E14307b

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96T

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ELISA Kit for Human PI3-kinase subunit alpha

Cat.:

E14307h

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96T

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ELISA Kit for Human PI3-kinase subunit alpha

Cat.:

E14307m

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96T

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CLIA Kit for Human PI3-kinase subunit alpha

Cat.:

U14307m

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96T

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CLIA Kit for Human PI3-kinase subunit alpha

Cat.:

U14307h

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Packing:

96T

CLIA Kit for Human PI3-kinase subunit alpha

MSDS:

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Polyclonal Antibody for Human PI3-kinase subunit alpha

Monoclonal Antibody for Human PI3-kinase subunit alpha

Protein for Human PI3-kinase subunit alpha

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

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Recovery

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Linearity

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References

1.

"Molecular cloning, cDNA sequence, and chromosomal localization of the human phosphatidylinositol 3-kinase p110 alpha (PIK3CA) gene."
Volinia S. , Hiles I. , Ormondroyd E. , Nizetic D. , Antonacci R. , Rocchi M. , Waterfield M.
Genomics24:472-477(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANTS VAL-43 AND ARG-332
2.

"Cloning and mutagenesis of the p110 alpha subunit of human phosphoinositide 3'-hydroxykinase."
Stirdivant S.M. , Ahern J. , Conroy R.R. , Barnett S.F. , Ledder L.M. , Oliff A. , Heimbrook D.C.
Bioorg. Med. Chem.5:65-74(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Lung.
4.

"Identification of a chromosome 3p14.3-21.1 gene, APPL, encoding an adaptor molecule that interacts with the oncoprotein-serine/threonine kinase AKT2."
Mitsuuchi Y. , Johnson S.W. , Sonoda G. , Tanno S. , Golemis E.A. , Testa J.R.
Oncogene18:4891-4898(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH APPL1
5.

"Phosphoinositide 3-kinase signaling pathway mediated by p110{alpha} regulates invadopodia formation."
Yamaguchi H. , Yoshida S. , Muroi E. , Yoshida N. , Kawamura M. , Kouchi Z. , Nakamura Y. , Sakai R. , Fukami K.
J. Cell Biol.193:1275-1288(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN INVADOPODIA FORMATION;CHARACTERIZATION OF VARIANTS LYS-545 AND ARG-1047
6.

"Insights into the oncogenic effects of PIK3CA mutations from the structure of p110alpha/p85alpha."
Huang C.-H. , Mandelker D. , Gabelli S.B. , Amzel L.M.
Cell Cycle7:1151-1156(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON CANCER
7.

"Class I PI3K in oncogenic cellular transformation."
Zhao L. , Vogt P.K.
Oncogene27:5486-5496(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION;REVIEW ON CANCER
8.

"Should individual PI3 kinase isoforms be targeted in cancer?"
Jia S. , Roberts T.M. , Zhao J.J.
Curr. Opin. Cell Biol.21:199-208(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION
9.

"The structure of a human p110alpha/p85alpha complex elucidates the effects of oncogenic PI3Kalpha mutations."
Huang C.-H. , Mandelker D. , Schmidt-Kittler O. , Samuels Y. , Velculescu V.E. , Kinzler K.W. , Vogelstein B. , Gabelli S.B. , Amzel L.M.
Science318:1744-1748(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3 ANGSTROMS) IN A COMPLEX WITH PIK3R1;DOMAINS
10.

"Solution structure of the C2 domain from human PI3-kinase p110 subunit alpha."
RIKEN structural genomics initiative (RSGI)
Submitted (2008-04) to the PDB data bank

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 331-481
11.

"A frequent kinase domain mutation that changes the interaction between PI3Kalpha and the membrane."
Mandelker D. , Gabelli S.B. , Schmidt-Kittler O. , Zhu J. , Cheong I. , Huang C.H. , Kinzler K.W. , Vogelstein B. , Amzel L.M.
Proc. Natl. Acad. Sci. U.S.A.106:16996-17001(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF MUTANT HIS-1047 IN COMPLEX WITH WORTMANNIN AND PIK3R1;INTERACTION WITH PIK3R1;CHARACTERIZATION OF VARIANT ARG-1047;DOMAINS
12.

"Mutations of PIK3CA in anaplastic oligodendrogliomas, high-grade astrocytomas, and medulloblastomas."
Broderick D.K. , Di C. , Parrett T.J. , Samuels Y.R. , Cummins J.M. , McLendon R.E. , Fults D.W. , Velculescu V.E. , Bigner D.D. , Yan H.
Cancer Res.64:5048-5050(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS CANCER LYS-542; LYS-545; PRO-546; ASN-1021; ARG-1047; LEU-1047 AND TYR-1065
13.

"Mutation of the PIK3CA gene in ovarian and breast cancer."
Campbell I.G. , Russell S.E. , Choong D.Y.H. , Montgomery K.G. , Ciavarella M.L. , Hooi C.S.F. , Cristiano B.E. , Pearson R.B. , Phillips W.A.
Cancer Res.64:7678-7681(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS CANCER GLY-545; LYS-545; LYS-546; GLU-546; ARG-1047 AND LEU-1047
14.

"High frequency of mutations of the PIK3CA gene in human cancers."
Samuels Y. , Wang Z. , Bardelli A. , Silliman N. , Ptak J. , Szabo S. , Yan H. , Gazdar A. , Powell S.M. , Riggins G.J. , Willson J.K.V. , Markowitz S. , Kinzler K.W. , Vogelstein B. , Velculescu V.E.
Science304:554-554(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT CANCER ARG-1047
15.

"PIK3CA mutations in glioblastoma multiforme."
Hartmann C. , Bartels G. , Gehlhaar C. , Holtkamp N. , von Deimling A.
Acta Neuropathol.109:639-642(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS CANCER GLN-88; LYS-542; ALA-545 AND ASN-1025
16.

"Mutations of PIK3CA in gastric adenocarcinoma."
Li V.S.W. , Wong C.W. , Chan T.L. , Chan A.S.W. , Zhao W. , Chu K.-M. , So S. , Chen X. , Yuen S.T. , Leung S.Y.
BMC Cancer5:29-29(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS CANCER LYS-542; LYS-545 AND ARG-1047
17.

"Functional analysis of PIK3CA gene mutations in human colorectal cancer."
Ikenoue T. , Kanai F. , Hikiba Y. , Obata T. , Tanaka Y. , Imamura J. , Ohta M. , Jazag A. , Guleng B. , Tateishi K. , Asaoka Y. , Matsumura M. , Kawabe T. , Omata M.
Cancer Res.65:4562-4567(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION OF VARIANTS CRC HIS-38; VAL-106; ARG-420; GLN-453; LYS-542; LYS-545; ILE-1043 AND ARG-1047
18.

"High frequency of coexistent mutations of PIK3CA and PTEN genes in endometrial carcinoma."
Oda K. , Stokoe D. , Taketani Y. , McCormick F.
Cancer Res.65:10669-10673(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS CANCER GLN-542; LYS-542; GLY-545; LYS-545; ARG-1007; HIS-1021; CYS-1021; VAL-1035; ILE-1043; TYR-1047; ARG-1047; ASP-1050; LYS-1052 AND LEU-1065
19.

"The prevalence of PIK3CA mutations in gastric and colon cancer."
Velho S. , Oliveira C. , Ferreira A. , Ferreira A.C. , Suriano G. , Schwartz S. Jr. , Duval A. , Carneiro F. , Machado J.C. , Hamelin R. , Seruca R.
Eur. J. Cancer41:1649-1654(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS CANCER LYS-542; GLY-545; LYS-545; GLN-1023; ARG-1047 AND LEU-1047
20.

"PIK3CA mutations in advanced ovarian carcinomas."
Wang Y. , Helland A. , Holm R. , Kristensen G.B. , Boerresen-Dale A.-L.
Hum. Mutat.25:322-322(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS CANCER LYS-545 AND ARG-1047
21.

"PIK3CA gene is frequently mutated in breast carcinomas and hepatocellular carcinomas."
Lee J.W. , Soung Y.H. , Kim S.Y. , Lee H.W. , Park W.S. , Nam S.W. , Kim S.H. , Lee J.Y. , Yoo N.J. , Lee S.H.
Oncogene24:1477-1480(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT HCC ALA-545
22.

"PIK3CA mutations in head and neck squamous cell carcinoma."
Qiu W. , Schoenleben F. , Li X. , Ho D.J. , Close L.G. , Manolidis S. , Bennett B.P. , Su G.H.
Clin. Cancer Res.12:1441-1446(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS CANCER CYS-343; LYS-542; LYS-545 AND ARG-1047;VARIANT MET-391
23.

"PIK3CA mutations in nasopharyngeal carcinoma."
Or Y.Y.-Y. , Hui A.B.-Y. , To K.-F. , Lam C.N.-Y. , Lo K.-W.
Int. J. Cancer118:1065-1067(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT CANCER ARG-1047
24.

"PIK3CA mutation and histological type in breast carcinoma: high frequency of mutations in lobular carcinoma."
Buttitta F. , Felicioni L. , Barassi F. , Martella C. , Paolizzi D. , Fresu G. , Salvatore S. , Cuccurullo F. , Mezzetti A. , Campani D. , Marchetti A.
J. Pathol.208:350-355(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS BC LYS-542; VAL-542; LYS-545; ARG-546; ARG-1047 AND LEU-1047
25.

"Cancer-specific mutations in PIK3CA are oncogenic in vivo."
Bader A.G. , Kang S. , Vogt P.K.
Proc. Natl. Acad. Sci. U.S.A.103:1475-1479(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION OF VARIANTS CANCER LYS-542; LYS-545 AND ARG-1047
26.

"Oncogenic PIK3CA mutations occur in epidermal nevi and seborrheic keratoses with a characteristic mutation pattern."
Hafner C. , Lopez-Knowles E. , Luis N.M. , Toll A. , Baselga E. , Fernandez-Casado A. , Hernandez S. , Ribe A. , Mentzel T. , Stoehr R. , Hofstaedter F. , Landthaler M. , Vogt T. , Pujol R.M. , Hartmann A. , Real F.X.
Proc. Natl. Acad. Sci. U.S.A.104:13450-13454(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS KERSEB LYS-542; LYS-545; GLY-545 AND ARG-1047
27.

"Somatic mosaic activating mutations in PIK3CA cause CLOVES syndrome."
Kurek K.C. , Luks V.L. , Ayturk U.M. , Alomari A.I. , Fishman S.J. , Spencer S.A. , Mulliken J.B. , Bowen M.E. , Yamamoto G.L. , Kozakewich H.P. , Warman M.L.
Am. J. Hum. Genet.90:1108-1115(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS CLOVE ARG-420; LYS-542 AND ARG-1047
28.

"De novo germline and postzygotic mutations in AKT3, PIK3R2 and PIK3CA cause a spectrum of related megalencephaly syndromes."
Riviere J.B. , Mirzaa G.M. , O'Roak B.J. , Beddaoui M. , Alcantara D. , Conway R.L. , St-Onge J. , Schwartzentruber J.A. , Gripp K.W. , Nikkel S.M. , Worthylake T. , Sullivan C.T. , Ward T.R. , Butler H.E. , Kramer N.A. , Albrecht B. , Armour C.M. , Armstrong L. , more...
Nat. Genet.44:934-940(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS MCAP LYS-81; GLN-88; ARG-364; LYS-365; TYR-378; GLU-453 DEL; LYS-545; LYS-726; ARG-914; CYS-1021; ALA-1025; VAL-1035; ILE-1043; TYR-1047 AND SER-1049
29.

"Germline PIK3CA and AKT1 mutations in Cowden and Cowden-like syndromes."
Orloff M.S. , He X. , Peterson C. , Chen F. , Chen J.L. , Mester J.L. , Eng C.
Am. J. Hum. Genet.92:76-80(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS CWS5 ASP-118; LYS-135; LYS-218; ILE-356; LYS-382 AND ALA-545

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