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Index > Protein center > TEC(Gene name) > Human
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  • TEC (Gene name),
  • Tyrosine-protein kinase Tec (Protein name ),  TEC_HUMAN from NCBI database.
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  • General Annotation
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  • Gene name:
    TEC(PSCTK4);
    Protein name:
    Tyrosine-protein kinase Tec;
    Alternative:

    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Interacts with INPP5D/SHIP1 and INPPL1/SHIP2. Interacts with CD28, FASLG, FGF2, GRB10 and KIT. Interact with VAV1 and JAK2.
    Function:
    Non-receptor tyrosine kinase that contributes to signaling from many receptors and participates as a signal transducer in multiple downstream pathways, including regulation of the actin cytoskekleton. Plays a redundant role to ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. Required for TCR-dependent IL2 gene induction. Phosphorylates DOK1, one CD28-specific substrate, and contributes to CD28-signaling. Mediates signals that negatively regulate IL2RA expression induced by TCR cross-linking. Plays a redundant role to BTK in BCR-signaling for B-cell development and activation, especially by phosphorylating STAP1, a BCR-signaling protein. Required in mast cells for efficient cytokine production. Involved in both growth and differentiation mechanisms of myeloid cells through activation by the granulocyte colony-stimulating factor CSF3, a critical cytokine to promoting the growth, differentiation, and functional activation of myeloid cells. Participates in platelet signaling downstream of integrin activation. Cooperates with JAK2 through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. GRB10, a negative modifier of the FOS activation pathway, is another substrate of TEC. TEC is involved in G protein-coupled receptor- and integrin-mediated signalings in blood platelets. Plays a role in hepatocyte proliferation and liver regeneration and is involved in HGF-induced ERK signaling pathway. TEC regulates also FGF2 unconventional secretion (endoplasmic reticulum (ER)/Golgi-independent mechanism) under various physiological conditions through phosphorylation of FGF2 'Tyr-215'. May also be involved in the regulation of osteoclast differentiation.
    Subcellular Location:
    Cytoplasm Cell membrane Peripheral membrane protein Cytoplasm cytoskeleton Following B-cell or T-cell receptors activation by antigen, translocates to the plasma membrane through its PH domain. Thrombin and integrin engagement induces translocation of TEC to the cytoskeleton during platelet activation. In cardiac myocytes, assumes a diffuse intracellular localization under basal conditions but is recruited to striated structures upon various stimuli, including ATP.
    Protein Attributes:
    Sequence length:
    631
    Sequence:
    50:
    MNFNTILEEI | LIKRSQQKKK | TSPLNYKERL | FVLTKSMLTY | YEGRAEKKYR | 
    100:
    KGFIDVSKIK | CVEIVKNDDG | VIPCQNKYPF | QVVHDANTLY | IFAPSPQSRD | 
    150:
    LWVKKLKEEI | KNNNNIMIKY | HPKFWTDGSY | QCCRQTEKLA | PGCEKYNLFE | 
    200:
    SSIRKALPPA | PETKKRRPPP | PIPLEEEDNS | EEIVVAMYDF | QAAEGHDLRL | 
    250:
    ERGQEYLILE | KNDVHWWRAR | DKYGNEGYIP | SNYVTGKKSN | NLDQYEWYCR | 
    300:
    NMNRSKAEQL | LRSEDKEGGF | MVRDSSQPGL | YTVSLYTKFG | GEGSSGFRHY | 
    350:
    HIKETTTSPK | KYYLAEKHAF | GSIPEIIEYH | KHNAAGLVTR | LRYPVSVKGK | 
    400:
    NAPTTAGFSY | EKWEINPSEL | TFMRELGSGL | FGVVRLGKWR | AQYKVAIKAI | 
    450:
    REGAMCEEDF | IEEAKVMMKL | THPKLVQLYG | VCTQQKPIYI | VTEFMERGCL | 
    500:
    LNFLRQRQGH | FSRDVLLSMC | QDVCEGMEYL | ERNSFIHRDL | AARNCLVSEA | 
    550:
    GVVKVSDFGM | ARYVLDDQYT | SSSGAKFPVK | WCPPEVFNYS | RFSSKSDVWS | 
    600:
    FGVLMWEVFT | EGRMPFEKYT | NYEVVTMVTR | GHRLYQPKLA | SNYVYEVMLR | 
    631:
    CWQEKPEGRP | SFEDLLRTID | ELVECEETFG | R
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    Pfam:
    UniGene:
    SMR:
    String:
    KEGG:
    MIM:
    Uniprot:
     
    FOR
    ELISA Kit for Human Tyrosine-protein kinase Tec
    Cat.:
    E8704h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Tyrosine-protein kinase Tec
    Cat.:
    E8704m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Tyrosine-protein kinase Tec
    Cat.:
    U8704h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Tyrosine-protein kinase Tec
    Cat.:
    U8704m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human Tyrosine-protein kinase Tec
    Cat.:
    P8704Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Tyrosine-protein kinase Tec
    Cat.:
    P8704Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human Tyrosine-protein kinase Tec
    Monoclonal Antibody for Human Tyrosine-protein kinase Tec
    Protein for Human Tyrosine-protein kinase Tec
    Protein for Human Tyrosine-protein kinase Tec

    R&D Technical Data

    s

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    s
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤7.6%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤8.9%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery

    Recovery was determined by spiking various levels of TEC into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    95

    91-99

    Plasma

    96

    93-101

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TEC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-99%

    90-101%

    91-98%

    92-97%

    EDTA plasma(n=5)

    90-102%

    93-100%

    89-104%

    93-98%

    heparin plasma(n=5)

    93-97%

     

    91-103%

    92-100%

    91-102%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      "Molecular cloning and analysis of the human Tec protein-tyrosine kinase."
      Sato K. , Mano H. , Ariyama T. , Inazawa J. , Yazaki Y. , Hirai H.
      Leukemia8:1663-1672(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Blood.
    2. 2.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    3. 3.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Liver.
    4. 4.
      "Identification of phosphorylation sites within the SH3 domains of Tec family tyrosine kinases."
      Nore B.F. , Mattsson P.T. , Antonsson P. , Backesjo C.-M. , Westlund A. , Lennartsson J. , Hansson H. , Low P. , Ronnstrand L. , Smith C.I.E.
      Biochim. Biophys. Acta1645:123-132(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 203-206;PHOSPHORYLATION AT TYR-206
    5. 5.
      "Tec kinase associates with c-kit and is tyrosine phosphorylated and activated following stem cell factor binding."
      Tang B. , Mano H. , Yi T. , Ihle J.N.
      Mol. Cell. Biol.14:8432-8437(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION;INTERACTION WITH KIT;ENZYME REGULATION
    6. 6.
      "Integrin-dependent tyrosine phosphorylation and cytoskeletal translocation of Tec in thrombin-activated platelets."
      Laffargue M. , Monnereau L. , Tuech J. , Ragab A. , Ragab-Thomas J. , Payrastre B. , Raynal P. , Chap H.
      Biochem. Biophys. Res. Commun.238:247-251(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION;SUBCELLULAR LOCATION
    7. 7.
      "SOCS-1/JAB/SSI-1 can bind to and suppress Tec protein-tyrosine kinase."
      Ohya K. , Kajigaya S. , Yamashita Y. , Miyazato A. , Hatake K. , Miura Y. , Ikeda U. , Shimada K. , Ozawa K. , Mano H.
      J. Biol. Chem.272:27178-27182(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ENZYME REGULATION
    8. 8.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH GRB10;FUNCTION IN PHOSPHORYLATION OF GRB10
    9. 9.
      "Tec is involved in G protein-coupled receptor- and integrin-mediated signalings in human blood platelets."
      Hamazaki Y. , Kojima H. , Mano H. , Nagata Y. , Todokoro K. , Abe T. , Nagasawa T.
      Oncogene16:2773-2779(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION;ENZYME REGULATION;SUBCELLULAR LOCATION
    10. 10.
      "Molecular cloning of a docking protein, BRDG1, that acts downstream of the Tec tyrosine kinase."
      Ohya K. , Kajigaya S. , Kitanaka A. , Yoshida K. , Miyazato A. , Yamashita Y. , Yamanaka T. , Ikeda U. , Shimada K. , Ozawa K. , Mano H.
      Proc. Natl. Acad. Sci. U.S.A.96:11976-11981(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF STAP1;ENZYME REGULATION
    11. 11.
      "SHIP family inositol phosphatases interact with and negatively regulate the Tec tyrosine kinase."
      Tomlinson M.G. , Heath V.L. , Turck C.W. , Watson S.P. , Weiss A.
      J. Biol. Chem.279:55089-55096(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH INPP5D AND INPPL1
    12. 12.
      "Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening."
      Voss M. , Lettau M. , Janssen O.
      BMC Immunol.10:53-53(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH FASLG
    13. 13.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-206 AND TYR-228;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    14. 14.
      "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
      Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-519;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Leukemic T-cell.
    15. 15.
      "Tec protein tyrosine kinase inhibits CD25 expression in human T-lymphocyte."
      Susaki K. , Kitanaka A. , Dobashi H. , Kubota Y. , Kittaka K. , Kameda T. , Yamaoka G. , Mano H. , Mihara K. , Ishida T.
      Immunol. Lett.127:135-142(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    16. 16.
      "Tec-kinase-mediated phosphorylation of fibroblast growth factor 2 is essential for unconventional secretion."
      Ebert A.D. , Laussmann M. , Wegehingel S. , Kaderali L. , Erfle H. , Reichert J. , Lechner J. , Beer H.D. , Pepperkok R. , Nickel W.
      Traffic11:813-826(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN PHOSPHORYLATION OF FGF2;INTERACTION WITH FGF2
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