Language:
  
[Sign in] [Register]   

EIAab logo

EIAab news detail, please contact eiaab@eiaab.com if you have any questions about online orders and payment.
Investigation of a Commercial ELISA for the Detection of Canine Procalcitonin
Update time:2014-02-18 21:51:00   【 Font: Large  Medium Small

 

Background

Rapid identification of sepsis enables prompt administration of antibiotics and is essential to improve patient survival. Procalcitonin (PCT) is a biomarker used to diagnose sepsis in people. Commercial assays to measure canine PCT peptide have not been validated.

Objective

To investigate the validity of a commercially available enzyme-linked immunosorbent assay (ELISA) marketed for the m

Animals

Three dogs with sepsis, 1 healthy dog, 1 dog with thyroid carcinoma.

easurement of canine PCT.

 

 

Methods

Experimental study. The ELISA's ability to detect recombinant and native canine PCT was investigated and intra-assay and interassay coefficients of variability were calculated. Assay validation including mass spectrometry of the kit standard solution was performed.

Results

The ELISA did not consistently detect recombinant canine PCT. Thyroid lysate yielded a positive ELISA signal. Intra-assay variability ranged from 18.9 to 77.4%, while interassay variability ranged from 56.1 to 79.5%. Mass spectrometry of the standard solution provided with the evaluated ELISA kit did not indicate presence of PCT.

Conclusions and Clinical Importance

The results of this investigation do not support the use of this ELISA for the detection of PCT in dogs.

Cited products
Source:Journal of Veterinary      by ANK Floras, MK Holowaychuk
Hot Genes
ALCAM ACE KSR2 ASPRO C19orf80 Gdf5 Trap1a Atf2
Top Searches
Ubiquitin-protein ligase Ubiquitin ELISA metalloproteinase Asprosin Tumor necrosis TRAP1A vitamin d
Why choose EIAAB
Our products have been quoted by many publications in famous journals such as Cell; Cell Metabolism; Hepatology; Biomaterials.more
Further Information
About us Protein center Bank account Distributors Terms & Conditions Career eiaab.cn

Copyright & copy www.eiaab.com2006-2016 All Rights Reserved    EIAab         Email:eiaab@eiaab.com

Twitter