Growth hormone plays an important role in growth control and is involved in the regulation of several anabolic processes. Implicated as an osmoregulatory substance important for seawater adaptation.
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s
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.5%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.3%
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.0%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.5%
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Recovery
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Recovery was determined by spiking various levels of GH1 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
108
105-110
Plasma
109
107-112
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Recovery was determined by spiking various levels of GH1 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
98-105
Plasma
98
96-103
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GH1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
105-110%
89-95%
97-106%
103-114%
EDTA plasma(n=5)
103-108%
109-113%
112-115%
96-105%
heparin plasma(n=5)
89-99%
109-115%
99-105%
106-112%
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GH1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
96-100%
87-95%
98-103%
106-109%
EDTA plasma(n=5)
97-99%
95-108%
103-110%
94-103%
heparin plasma(n=5)
97-106%
94-99%
89-96%
96-104%
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Vaild Sequence:
