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Index > Protein center > ORM1(Gene name) > Human
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  • ORM1 (Gene name),
  • Alpha-1-acid glycoprotein 1 (Protein name ),  A1AG1_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Gene name:
    ORM1(AGP1);
    Protein name:
    Alpha-1-acid glycoprotein 1(AGP 1);
    Alternative:
    Orosomucoid;Orosomucoid-1(OMD 1);ORM;
    Organism:
    ORM1 (Homo sapiens). 
    General Annotation
    Sub Unit:
    Function:
    Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    201
    Sequence:
    50:
    MALSWVLTVL | SLLPLLEAQI | PLCANLVPVP | ITNATLDQIT | GKWFYIASAF | 
    100:
    RNEEYNKSVQ | EIQATFFYFT | PNKTEDTIFL | REYQTRQDQC | IYNTTYLNVQ | 
    150:
    RENGTISRYV | GGQEHFAHLL | ILRDTKTYML | AFDVNDEKNW | GLSVYADKPE | 
    200:
    TTKEQLGEFY | EALDCLRIPK | SDVVYTDWKK | DKCEPLEKQH | EKERKQEEGE | 
    201:
    S
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    String:
    Pfam:
    MIM:
    UniGene:
    SMR:
    KEGG:
    Uniprot:
     
    FOR
    ELISA Kit for Human AGP 1
    Cat.:
    E0816h
    Price:
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Human AGP 1
    Cat.:
    E0816m
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Human AGP 1
    Cat.:
    E0816r
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Human AGP 1
    Cat.:
    E0816b
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Human AGP 1
    MSDS:
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    CLIA Kit for Human AGP 1
    Cat.:
    U0816h
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human AGP 1
    Cat.:
    U0816b
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human AGP 1
    MSDS:
    Please sign in first.
    CLIA Kit for Human AGP 1
    Cat.:
    U0816r
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    MSDS:
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    Packing:
    96T
    Range:
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    CLIA Kit for Human AGP 1
    Cat.:
    U0816m
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    MSDS:
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    Packing:
    96T
    Polyclonal Antibody for Human AGP 1
    Cat.:
    P0816Rb-m
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human AGP 1
    Polyclonal Antibody for Human AGP 1
    Cat.:
    P0816Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human AGP 1
    Polyclonal Antibody for Human AGP 1
    Cat.:
    P0816Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human AGP 1
    Monoclonal Antibody for Human AGP 1
    Monoclonal Antibody for Human AGP 1
    Monoclonal Antibody for Human AGP 1
    Monoclonal Antibody for Human AGP 1
    Protein for Human AGP 1
    Protein for Human AGP 1
    Protein for Human AGP 1
    Protein for Human AGP 1
    Protein for Human AGP 1
    Cat.:
    R0816h
    Price:
    Please sign in first.
    Packing:
    1mg
    Purity:
    >90%

    R&D Technical Data

     12% SDS-PAGE analysis

              12% SDS-PAGE analysis

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    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤7.6%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤11.3%

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    Recovery
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    Recovery was determined by spiking various levels of A1AG into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    102

    99-106

    Plasma

    101

    95-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of A1AG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    97-106%

    98-107%

    98-107%

    97-107%

    EDTA plasma(n=5)

    100-104%

    95-109%

    99-105%

    96-108%

    heparin plasma(n=5)

    99-105%

     

    99-106%

    97-105%

    99-105%

     

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    References
    1. 1.
      "Structure and expression of the genes coding for human alpha 1-acid glycoprotein."
      Dente L. , Pizza M.G. , Metspalu A. , Cortese R.
      EMBO J.6:2289-2296(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    2. 2.
      "Molecular cloning and nucleotide sequence of human alpha 1 acid glycoprotein cDNA."
      Board P.G. , Jones I.M. , Bentley A.K.
      Gene44:127-131(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    3. 3.
      "Structure of the human alpha 1-acid glycoprotein gene: sequence homology with other human acute phase protein genes."
      Dente L. , Ciliberto G. , Cortese R.
      Nucleic Acids Res.13:3941-3952(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    4. 4.
      "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
      Submitted (2004-10) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    5. 5.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Liver.
    6. 6.
      "DNA sequence and analysis of human chromosome 9."
      Humphray S.J. , Oliver K. , Hunt A.R. , Plumb R.W. , Loveland J.E. , Howe K.L. , Andrews T.D. , Searle S. , Hunt S.E. , Scott C.E. , Jones M.C. , Ainscough R. , Almeida J.P. , Ambrose K.D. , Ashwell R.I.S. , Babbage A.K. , Babbage S. , Bagguley C.L. , more...
      Nature429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA];VARIANT ARG-38
    7. 7.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA];VARIANT ARG-38
    8. 8.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANTS ARG-38; CYS-167 AND MET-174
      tissue: Liver.
    9. 9.
      "Structure of alpha 1-acid glycoprotein. The complete amino acid sequence, multiple amino acid substitutions, and homology with the immunoglobulins."
      Schmid K. , Kaufmann H. , Isemura S. , Bauer F. , Emura J. , Motoyama T. , Ishiguro M. , Nanno S.
      Biochemistry12:2711-2724(1973) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 19-129;PYROGLUTAMATE FORMATION AT GLN-19
    10. 10.
      "Isolation and partial characterization of the cyanogen bromide fragments of alpha 1-acid glycoprotein and the elucidation of the amino acid sequence of the carboxyl-terminal cyanogen bromide fragment."
      Ikenaka T. , Ishiguro M. , Emura J. , Kaufmann H. , Isemura S. , Bauer W. , Schmid K.
      Biochemistry11:3817-3829(1972) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 129-201
    11. 11.
      "The disulfide bonds of alpha1-acid glycoprotein."
      Schmid K. , Buergi W. , Collins J.H. , Nanno S.
      Biochemistry13:2694-2697(1974) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISULFIDE BONDS
    12. 12.
      "Analysis of the five glycosylation sites of human alpha 1-acid glycoprotein."
      Treuheit M.J. , Costello C.E. , Halsall H.B.
      Biochem. J.283:105-112(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT ASN-33; ASN-56; ASN-72; ASN-93 AND ASN-103
    13. 13.
      "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry."
      Zhang H. , Li X.-J. , Martin D.B. , Aebersold R.
      Nat. Biotechnol.21:660-666(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT ASN-33
    14. 14.
      "A new strategy for identification of N-glycosylated proteins and unambiguous assignment of their glycosylation sites using HILIC enrichment and partial deglycosylation."
      Hagglund P. , Bunkenborg J. , Elortza F. , Jensen O.N. , Roepstorff P.
      J. Proteome Res.3:556-566(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT ASN-33; ASN-72 AND ASN-93;IDENTIFICATION BY MASS SPECTROMETRY
    15. 15.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33 AND ASN-93
      tissue: Bile.
    16. 16.
      "Screening for N-glycosylated proteins by liquid chromatography mass spectrometry."
      Bunkenborg J. , Pilch B.J. , Podtelejnikov A.V. , Wisniewski J.R.
      Proteomics4:454-465(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33; ASN-56; ASN-72; ASN-93 AND ASN-103
      tissue: Plasma.
    17. 17.
      "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
      Liu T. , Qian W.-J. , Gritsenko M.A. , Camp D.G. II , Monroe M.E. , Moore R.J. , Smith R.D.
      J. Proteome Res.4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33; ASN-56; ASN-72; ASN-93 AND ASN-103
      tissue: Plasma.
    18. 18.
      "Selective binding of imatinib to the genetic variants of human alpha1-acid glycoprotein."
      Fitos I. , Visy J. , Zsila F. , Mady G. , Simonyi M.
      Biochim. Biophys. Acta1760:1704-1712(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    19. 19.
      "Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."
      Ramachandran P. , Boontheung P. , Xie Y. , Sondej M. , Wong D.T. , Loo J.A.
      J. Proteome Res.5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-93
      tissue: Saliva.
    20. 20.
      "The drug binding site of human alpha1-acid glycoprotein: insight from induced circular dichroism and electronic absorption spectra."
      Zsila F. , Iwao Y.
      Biochim. Biophys. Acta1770:797-809(2007) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    21. 21.
      "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
      Chen R. , Jiang X. , Sun D. , Han G. , Wang F. , Ye M. , Wang L. , Zou H.
      J. Proteome Res.8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-93 AND ASN-103
      tissue: Liver.
    22. 22.
      "Enrichment of glycopeptides for glycan structure and attachment site identification."
      Nilsson J. , Rueetschi U. , Halim A. , Hesse C. , Carlsohn E. , Brinkmalm G. , Larson G.
      Nat. Methods6:809-811(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33 AND ASN-72;STRUCTURE OF CARBOHYDRATES
      tissue: Cerebrospinal fluid.
    23. 23.
      "Human urinary glycoproteomics; attachment site specific analysis of N-and O-linked glycosylations by CID and ECD."
      Halim A. , Nilsson J. , Ruetschi U. , Hesse C. , Larson G.
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      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT ASN-33;STRUCTURE OF CARBOHYDRATES;IDENTIFICATION BY MASS SPECTROMETRY
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      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 20-201;DOMAIN;DISULFIDE BONDS
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      "Human orosomucoid polymorphism: molecular basis of the three common ORM1 alleles, ORM1*F1, ORM1*F2, and ORM1*S."
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      Hum. Genet.99:393-398(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS ARG-38 AND MET-174
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