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Index > Protein center > ADORA1(Gene name) > Human
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  • ADORA1 (Gene name),
  • Adenosine receptor A1 (Protein name ),  AA1R_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    ADORA1;
    Protein name:
    Adenosine receptor A1;
    Alternative:

    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
    Subcellular Location:
    Cell membrane Multi-pass membrane protein
    Protein Attributes:
    Sequence length:
    326
    Sequence:
    50:
    MPPSISAFQA | AYIGIEVLIA | LVSVPGNVLV | IWAVKVNQAL | RDATFCFIVS | 
    100:
    LAVADVAVGA | LVIPLAILIN | IGPQTYFHTC | LMVACPVLIL | TQSSILALLA | 
    150:
    IAVDRYLRVK | IPLRYKMVVT | PRRAAVAIAG | CWILSFVVGL | TPMFGWNNLS | 
    200:
    AVERAWAANG | SMGEPVIKCE | FEKVISMEYM | VYFNFFVWVL | PPLLLMVLIY | 
    250:
    LEVFYLIRKQ | LNKKVSASSG | DPQKYYGKEL | KIAKSLALIL | FLFALSWLPL | 
    300:
    HILNCITLFC | PSCHKPSILT | YIAIFLTHGN | SAMNPIVYAF | RIQKFRVTFL | 
    326:
    KIWNDHFRCQ | PAPPIDEDLP | EERPDD
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    SMR:
    KEGG:
    UniGene:
    MIM:
    Pfam:
    String:
    Uniprot:
     
    FOR
    ELISA Kit for Human Adenosine receptor A1
    Cat.:
    E12273h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Adenosine receptor A1
    MSDS:
    Please sign in first.
    ELISA Kit for Human Adenosine receptor A1
    MSDS:
    Please sign in first.
    ELISA Kit for Human Adenosine receptor A1
    MSDS:
    Please sign in first.
    ELISA Kit for Human Adenosine receptor A1
    Cat.:
    E12273m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human Adenosine receptor A1
    Cat.:
    E12273r
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human Adenosine receptor A1
    MSDS:
    Please sign in first.
    CLIA Kit for Human Adenosine receptor A1
    MSDS:
    Please sign in first.
    CLIA Kit for Human Adenosine receptor A1
    MSDS:
    Please sign in first.
    CLIA Kit for Human Adenosine receptor A1
    MSDS:
    Please sign in first.
    CLIA Kit for Human Adenosine receptor A1
    MSDS:
    Please sign in first.
    CLIA Kit for Human Adenosine receptor A1
    Cat.:
    U12273h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Adenosine receptor A1
    Cat.:
    U12273m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Adenosine receptor A1
    Cat.:
    U12273r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human Adenosine receptor A1
    Polyclonal Antibody for Human Adenosine receptor A1
    Polyclonal Antibody for Human Adenosine receptor A1
    Polyclonal Antibody for Human Adenosine receptor A1
    Cat.:
    P12273Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Adenosine receptor A1
    Polyclonal Antibody for Human Adenosine receptor A1
    Polyclonal Antibody for Human Adenosine receptor A1
    Monoclonal Antibody for Human Adenosine receptor A1
    Monoclonal Antibody for Human Adenosine receptor A1
    Monoclonal Antibody for Human Adenosine receptor A1
    Monoclonal Antibody for Human Adenosine receptor A1
    Monoclonal Antibody for Human Adenosine receptor A1
    Monoclonal Antibody for Human Adenosine receptor A1
    Monoclonal Antibody for Human Adenosine receptor A1
    Protein for Human Adenosine receptor A1
    Protein for Human Adenosine receptor A1
    Protein for Human Adenosine receptor A1
    Protein for Human Adenosine receptor A1
    Protein for Human Adenosine receptor A1
    Protein for Human Adenosine receptor A1
    Protein for Human Adenosine receptor A1

    R&D Technical Data

    s

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    s
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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤8.2%

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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery

    Recovery was determined by spiking various levels of AA1R into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    101

    93-104

    Plasma

    100

    92-106

     

     

     

     

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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AA1R and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    94-108%

    97-106%

    95-106%

    92-110%

    EDTA plasma(n=5)

    92-107%

    95-105%

    91-108%

    94-105%

    heparin plasma(n=5)

    95-103%

     

    93-108%

    93-107%

    90-110%

     

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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    References
    1. 1.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Hippocampus.
    2. 2.
      "Molecular cloning and characterisation of a human brain A1 adenosine receptor cDNA."
      Townsend-Nicholson A. , Shine J.
      Brain Res. Mol. Brain Res.16:365-370(1992) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Brain.
    3. 3.
      "Characterization of the human A1 adenosine receptor gene. Evidence for alternative splicing."
      Ren H. , Stiles G.L.
      J. Biol. Chem.269:3104-3110(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Hippocampus.
    4. 4.
      Salvatore C.A. , Luneau C.J. , Johnson R.G. , Jacobson M.
      Submitted (1992-09) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Brain.
    5. 5.
      "Complete nucleotide sequence of an adenosine A1-receptor from heart."
      Hirabatake Y. , Takao K. , Hagiwara S. , Kasanuki H. , Hosoda S. , Kokubun S.
      Submitted (1997-06) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
      tissue: Heart.
    6. 6.
      "Identification of a 3TM adenosine receptor subtype 1 variant."
      Feng Y.-H. , Cheng H. , Qiu R.
      Submitted (2006-10) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)
    7. 7.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Brain.
      tissue: Hippocampus.
    8. 8.
      "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III , Ikeda S.R. , Aronstam R.S.
      Submitted (2002-07) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
      tissue: Brain.
    9. 9.
      "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
      Submitted (2003-05) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
    10. 10.
      "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L. , Schick M. , Neubert P. , Schatten R. , Henze S. , Korn B.
      Submitted (2004-06) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
    11. 11.
      "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G. , Barlow K.F. , McLay K.E. , Kaul R. , Swarbreck D. , Dunham A. , Scott C.E. , Howe K.L. , Woodfine K. , Spencer C.C.A. , Jones M.C. , Gillson C. , Searle S. , Zhou Y. , Kokocinski F. , McDonald L. , Evans R. , Phillips K. , more...
      Nature441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    12. 12.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    13. 13.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1);VARIANTS SER-43; PRO-50; HIS-105 AND GLN-261
      tissue: Brain.
    14. 14.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT [LARGE SCALE ANALYSIS] LYS-170
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