Adora1 ELISA Kit for Rat(Rattus norvegicus) | EIAab.comAdora1 ELISA Kit for Rat(Rattus norvegicus) | EIAab.com

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Adora1 (Gene name),

Adenosine receptor A1 (Protein name ), AA1R_RAT from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Citations (1)

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Gene name:

Adora1;

Protein name:

Adenosine receptor A1;

Alternative:

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

N/A

Function:

Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.

Subcellular Location:

Cell membrane Multi-pass membrane protein

Protein Attributes:

Sequence length:

326

Sequence:

50:

MPPYISAFQA | AYIGIEVLIA | LVSVPGNVLV | IWAVKVNQAL | RDATFCFIVS |

100:

LAVADVAVGA | LVIPLAILIN | IGPQTYFHTC | LMVACPVLIL | TQSSILALLA |

150:

IAVDRYLRVK | IPLRYKTVVT | QRRAAVAIAG | CWILSLVVGL | TPMFGWNNLS |

200:

VVEQDWRANG | SVGEPVIKCE | FEKVISMEYM | VYFNFFVWVL | PPLLLMVLIY |

250:

LEVFYLIRKQ | LNKKVSASSG | DPQKYYGKEL | KIAKSLALIL | FLFALSWLPL |

300:

HILNCITLFC | PTCQKPSILI | YIAIFLTHGN | SAMNPIVYAF | RIHKFRVTFL |

326:

KIWNDHFRCQ | PKPPIDEDLP | EEKAED

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

UniGene:

Pfam:

KEGG:

Uniprot:

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ELISA Kit for Rat Adenosine receptor A1

Cat.:

E12273h

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ELISA Kit for Rat Adenosine receptor A1

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ELISA Kit for Rat Adenosine receptor A1

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ELISA Kit for Rat Adenosine receptor A1

MSDS:

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ELISA Kit for Rat Adenosine receptor A1

Cat.:

E12273m

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ELISA Kit for Rat Adenosine receptor A1

Cat.:

E12273r

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96T

ELISA Kit for Rat Adenosine receptor A1

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CLIA Kit for Rat Adenosine receptor A1

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CLIA Kit for Rat Adenosine receptor A1

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CLIA Kit for Rat Adenosine receptor A1

MSDS:

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CLIA Kit for Rat Adenosine receptor A1

MSDS:

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CLIA Kit for Rat Adenosine receptor A1

Cat.:

U12273h

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CLIA Kit for Rat Adenosine receptor A1

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U12273m

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96T

CLIA Kit for Rat Adenosine receptor A1

Cat.:

U12273r

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Polyclonal Antibody for Rat Adenosine receptor A1

Polyclonal Antibody for Rat Adenosine receptor A1

Polyclonal Antibody for Rat Adenosine receptor A1

Polyclonal Antibody for Rat Adenosine receptor A1

Cat.:

P12273Rb-h

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40ug/0.2ml

Polyclonal Antibody for Rat Adenosine receptor A1

Polyclonal Antibody for Rat Adenosine receptor A1

Polyclonal Antibody for Rat Adenosine receptor A1

Monoclonal Antibody for Rat Adenosine receptor A1

Monoclonal Antibody for Rat Adenosine receptor A1

Monoclonal Antibody for Rat Adenosine receptor A1

Monoclonal Antibody for Rat Adenosine receptor A1

Monoclonal Antibody for Rat Adenosine receptor A1

Monoclonal Antibody for Rat Adenosine receptor A1

Monoclonal Antibody for Rat Adenosine receptor A1

Protein for Rat Adenosine receptor A1

Protein for Rat Adenosine receptor A1

Protein for Rat Adenosine receptor A1

Protein for Rat Adenosine receptor A1

Protein for Rat Adenosine receptor A1

Protein for Rat Adenosine receptor A1

Protein for Rat Adenosine receptor A1

R&D Technical Data

s

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s

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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.2%

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

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Recovery

Recovery was determined by spiking various levels of AA1R into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	101	93-104
Plasma	100	92-106

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AA1R and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	94-108%	97-106%	95-106%	92-110%
EDTA plasma(n=5)	92-107%	95-105%	91-108%	94-105%
heparin plasma(n=5)	95-103%	93-108%	93-107%	90-110%

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

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Citations

[1].

"The Ying and Yang of Adenosine A1 and A2A Receptors on ERK1/2 Activation in a Rat Model of Global Cerebral Ischemia Reperfusion Injury"

R Atef, AM Agha1, ARA Abdel-Rhaman, et al.

From:Mol Neurobiol

References

1.

"Molecular cloning and characterization of a rat A1-adenosine receptor that is widely expressed in brain and spinal cord."
Reppert S.M. , Weaver D.R. , Stehle J.H. , Rivkees S.A.
Mol. Endocrinol.5:1037-1048(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"Cloning and expression of an A1 adenosine receptor from rat brain."
Mahan L.C. , McVittie L.D. , Smyk-Randall E.M. , Nakata H. , Monsma F.J. Jr. , Gerfen C.R. , Sibley D.R.
Mol. Pharmacol.40:1-7(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Brain.
3.

Hirabatake Y. , Takao K. , Hagiwara S. , Kasanuki H. , Hosoda S. , Kokubun S.
Submitted (1997-03) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Heart.
4.

Yip L. , Mourelatos K. , Hewitt J. , Kwok Y.N.
Submitted (1998-01) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Wistar.

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