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Index > Protein center > Adipoq(Gene name) > Mouse
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  • Adipoq (Gene name),
  • Adiponectin (Protein name ),  ADIPO_MOUSE from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    Adipoq(Acdc;Acrp30;Apm1);
    Protein name:
    Adiponectin;
    Alternative:
    Adipocyte complement-related 30 kDa protein(ACRP30);30 kDa adipocyte complement-related protein;Adipocyte-specific protein AdipoQ;Adipocyte, C1q and collagen domain-containing protein;
    Organism:
    Mouse (Mus musculus). 
    General Annotation
    Sub Unit:
    Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2. Interacts with CTRP9 via the C1q domain (heterotrimeric complex).
    Function:
    Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    247
    Sequence:
    50:
    MLLLQALLFL | LILPSHAEDD | VTTTEELAPA | LVPPPKGTCA | GWMAGIPGHP | 
    100:
    GHNGTPGRDG | RDGTPGEKGE | KGDAGLLGPK | GETGDVGMTG | AEGPRGFPGT | 
    150:
    PGRKGEPGEA | AYMYRSAFSV | GLETRVTVPN | VPIRFTKIFY | NQQNHYDGST | 
    200:
    GKFYCNIPGL | YYFSYHITVY | MKDVKVSLFK | KDKAVLFTYD | QYQEKNVDQA | 
    247:
    SGSVLLHLEV | GDQVWLQVYG | DGDHNGLYAD | NVNDSTFTGF | LLYHDTN
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    String:
    UniGene:
    Pfam:
    KEGG:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Mouse Adiponectin
    Cat.:
    E0605h
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Mouse Adiponectin
    Cat.:
    E0605m
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Mouse Adiponectin
    Cat.:
    E0605d
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Mouse Adiponectin
    Cat.:
    E0605b
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Mouse Adiponectin
    Cat.:
    E0605r
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    MSDS:
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    Packing:
    96T
    Range:
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    CLIA Kit for Mouse Adiponectin
    Cat.:
    U0605b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse Adiponectin
    Cat.:
    U0605d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Mouse Adiponectin
    Cat.:
    U0605r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse Adiponectin
    Cat.:
    U0605h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Mouse Adiponectin
    Cat.:
    U0605m
    Price:
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    MSDS:
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    Packing:
    96T
    Polyclonal Antibody for Mouse Adiponectin
    Cat.:
    P0605Rb-m
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse Adiponectin
    Polyclonal Antibody for Mouse Adiponectin
    Cat.:
    P0605Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Mouse Adiponectin
    Polyclonal Antibody for Mouse Adiponectin
    Cat.:
    P0605Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Mouse Adiponectin
    Monoclonal Antibody for Mouse Adiponectin
    Monoclonal Antibody for Mouse Adiponectin
    Monoclonal Antibody for Mouse Adiponectin
    Monoclonal Antibody for Mouse Adiponectin
    Protein for Mouse Adiponectin
    Protein for Mouse Adiponectin
    Protein for Mouse Adiponectin
    Protein for Mouse Adiponectin
    Protein for Mouse Adiponectin
    Cat.:
    R0605h
    Price:
    Please sign in first.
    Packing:
    1mg
    Purity:
    >90%

    R&D Technical Data
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               12% SDS-PAGE Analysis

     

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤ 4.5%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤ 7.1%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    95

    91-98

    Plasma

    97

    92-102

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    87-95%

    90-101%

    92-103%

    90-98%

    EDTA plasma(n=5)

    89-95%

    91-102%

    89-101%

    90-102%

    heparin plasma(n=5)

    90-98%

     

    92-105%

    96-101%

    89-99%

     

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    References
    1. 1.
      "A novel serum protein similar to C1q, produced exclusively in adipocytes."
      Scherer P.E. , Williams S. , Fogliano M. , Baldini G. , Lodish H.F.
      J. Biol. Chem.270:26746-26749(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Adipocyte.
    2. 2.
      "AdipoQ is a novel adipose-specific gene dysregulated in obesity."
      Hu E. , Liang P. , Spiegelman B.M.
      J. Biol. Chem.271:10697-10703(1996) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      tissue: Fibroblast.
    3. 3.
      "Chromosomal localization, expression pattern, and promoter analysis of the mouse gene encoding adipocyte-specific secretory protein Acrp30."
      Das K. , Lin Y. , Widen E. , Zhang Y. , Scherer P.E.
      Biochem. Biophys. Res. Commun.280:1120-1129(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    4. 4.
      "Cloning of murine adipocyte complement-related protein of 30 KDa from white adipose tissue."
      Wang S.F. , Han P.Z. , Mu C.J. , Zhao M.H.
      Submitted (2004-09) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
      strain: C57BL/6J.
      strain: IRM-2.
      tissue: White adipose tissue.
    5. 5.
      "The transcriptional landscape of the mammalian genome."
      Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
      Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: C57BL/6J.
      tissue: Heart.
      tissue: Thymus.
    6. 6.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
      strain: C57BL/6J.
    7. 7.
      Mural R.J. , Adams M.D. , Myers E.W. , Smith H.O. , Venter J.C.
      Submitted (2005-07) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    8. 8.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      strain: C57BL/6J.
      tissue: Thymus.
    9. 9.
      "Hydroxylation and glycosylation of the four conserved lysine residues in the collagenous domain of adiponectin. Potential role in the modulation of its insulin-sensitizing activity."
      Wang Y. , Xu A. , Knight C. , Xu L.Y. , Cooper G.J.S.
      J. Biol. Chem.277:19521-19529(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 18-25;HYDROXYLATION AT LYS-68; LYS-71; LYS-80; PRO-94 AND LYS-104;GLYCOSYLATION AT LYS-68; LYS-71; LYS-80 AND LYS-104;GLYCAN STRUCTURE;ABSENCE OF HYDROXYLATION AT PRO-79; PRO-98 AND PRO-107;ABSENCE OF GLYCOSYLATION AT ASN-233;IDENTIFICATION BY MASS SPECTROMETRY
    10. 10.
      "Identification and adipocyte differentiation-dependent expression of the unique disialic acid residue in an adipose tissue-specific glycoprotein, adipo Q."
      Sato C. , Yasukawa Z. , Honda N. , Matsuda T. , Kitajima K.
      J. Biol. Chem.276:28849-28856(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE OF CARBOHYDRATES
    11. 11.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    12. 12.
      "The adipocyte-secreted protein Acrp30 enhances hepatic insulin action."
      Berg A.H. , Combs T.P. , Du X. , Brownlee M. , Scherer P.E.
      Nat. Med.7:947-953(2001) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    13. 13.
      "The fat-derived hormone adiponectin alleviates alcoholic and nonalcoholic fatty liver diseases in mice."
      Xu A. , Wang Y. , Keshaw H. , Xu L.Y. , Lam K.S.L. , Cooper G.J.S.
      J. Clin. Invest.112:91-100(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    14. 14.
      "Testosterone selectively reduces the high molecular weight form of adiponectin by inhibiting its secretion from adipocytes."
      Xu A. , Chan K.W. , Hoo R.L.C. , Wang Y. , Tan K.C.B. , Zhang J. , Chen B. , Lam M.C. , Tse C. , Cooper G.J.S. , Lam K.S.L.
      J. Biol. Chem.280:18073-18080(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBUNIT;FUNCTION
    15. 15.
      "Adiponectin inhibits cell proliferation by interacting with several growth factors in an oligomerization-dependent manner."
      Wang Y. , Lam K.S.L. , Xu J.Y. , Lu G. , Xu L.Y. , Cooper G.J.S. , Xu A.
      J. Biol. Chem.280:18341-18347(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBUNIT;FUNCTION
    16. 16.
      "Post-translational modifications of the four conserved lysine residues within the collagenous domain of adiponectin are required for the formation of its high molecular weight oligomeric complex."
      Wang Y. , Lam K.S.L. , Chan L. , Chan K.W. , Lam J.B.B. , Lam M.C. , Hoo R.C.L. , Mak W.W.N. , Cooper G.J.S. , Xu A.
      J. Biol. Chem.281:16391-16400(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBUNIT;MUTAGENESIS OF LYS-68; LYS-71; LYS-80 AND LYS-104
    17. 17.
      "Identification and characterization of CTRP9, a novel secreted glycoprotein, from adipose tissue that reduces serum glucose in mice and forms heterotrimers with adiponectin."
      Wong G.W. , Krawczyk S.A. , Kitidis-Mitrokostas C. , Ge G. , Spooner E. , Hug C. , Gimeno R. , Lodish H.F.
      FASEB J.23:241-258(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBUNIT;INTERACTION WITH CTRP9;MUTAGENESIS OF CYS-39
    18. 18.
      "Sialic acid modification of adiponectin is not required for multimerization or secretion but determines half-life in circulation."
      Richards A.A. , Colgrave M.L. , Zhang J. , Webster J. , Simpson F. , Preston E. , Wilks D. , Hoehn K.L. , Stephenson M. , Macdonald G.A. , Prins J.B. , Cooney G.J. , Xu A. , Whitehead J.P.
      Mol. Endocrinol.24:229-239(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT THR-23 AND THR-24;SUBUNIT
    19. 19.
      "The crystal structure of a complement-1q family protein suggests an evolutionary link to tumor necrosis factor."
      Shapiro L. , Scherer P.E.
      Curr. Biol.8:335-338(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 111-247
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