Gene name:
AIFM1 (AIF;PDCD8) ;
Protein name:
Apoptosis-inducing factor 1, mitochondrial ;
Alternative:
Programmed cell death protein 8 ;
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
Interacts with XIAP. Interacts (via N-terminus) with EIF3G (via C-terminus).
Function:
Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
Subcellular Location:
Mitochondrion intermembrane space
Mitochondrion inner membrane
Cytoplasm
Nucleus
Cytoplasm
perinuclear region
Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.
Protein Attributes:
50:
MFRCGGLAAG | ALKQKLVPLV | RTVCVRSPRQ | RNRLPGNLFQ | RWHVPLELQM |
100:
TRQMASSGAS | GGKIDNSVLV | LIVGLSTVGA | GAYAYKTMKE | DEKRYNERIS |
150:
GLGLTPEQKQ | KKAALSASEG | EEVPQDKAPS | HVPFLLIGGG | TAAFAAARSI |
200:
RARDPGARVL | IVSEDPELPY | MRPPLSKELW | FSDDPNVTKT | LRFKQWNGKE |
250:
RSIYFQPPSF | YVSAQDLPHI | ENGGVAVLTG | KKVVQLDVRD | NMVKLNDGSQ |
300:
ITYEKCLIAT | GGTPRSLSAI | DRAGAEVKSR | TTLFRKIGDF | RSLEKISREV |
350:
KSITIIGGGF | LGSELACALG | RKARALGTEV | IQLFPEKGNM | GKILPEYLSN |
400:
WTMEKVRREG | VKVMPNAIVQ | SVGVSSGKLL | IKLKDGRKVE | TDHIVAAVGL |
450:
EPNVELAKTG | GLEIDSDFGG | FRVNAELQAR | SNIWVAGDAA | CFYDIKLGRR |
500:
RVEHHDHAVV | SGRLAGENMT | GAAKPYWHQS | MFWSDLGPDV | GYEAIGLVDS |
550:
SLPTVGVFAK | ATAQDNPKSA | TEQSGTGIRS | ESETESEASE | ITIPPSTPAV |
600:
PQAPVQGEDY | GKGVIFYLRD | KVVVGIVLWN | IFNRMPIARK | IIKDGEQHED |
Vaild Sequence:
Related Databases
Uniprot:
ELISA Kit
CLIA Kit
Polyclonal Antibody
Monoclonal Antibody
Protein
FOR
Human
Rat
Mouse
ELISA Kit for Human Apoptosis-inducing factor 1, mitochondrial
ELISA Kit for Human Apoptosis-inducing factor 1, mitochondrial
ELISA Kit for Human Apoptosis-inducing factor 1, mitochondrial
CLIA Kit for Human Apoptosis-inducing factor 1, mitochondrial
CLIA Kit for Human Apoptosis-inducing factor 1, mitochondrial
CLIA Kit for Human Apoptosis-inducing factor 1, mitochondrial
Polyclonal Antibody for Human Apoptosis-inducing factor 1, mitochondrial
Polyclonal Antibody for Human Apoptosis-inducing factor 1, mitochondrial
Polyclonal Antibody for Human Apoptosis-inducing factor 1, mitochondrial
Monoclonal Antibody for Human Apoptosis-inducing factor 1, mitochondrial
Monoclonal Antibody for Human Apoptosis-inducing factor 1, mitochondrial
Monoclonal Antibody for Human Apoptosis-inducing factor 1, mitochondrial
Protein for Human Apoptosis-inducing factor 1, mitochondrial
Protein for Human Apoptosis-inducing factor 1, mitochondrial
Protein for Human Apoptosis-inducing factor 1, mitochondrial
R&D Technical Data
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Precision
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Recovery
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Linearity
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
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Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3)
tissue :
Kidney .
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
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Shatkay H.
,
Dew I.
,
Miller J.R.
,
Flanigan M.J.
,
Edwards N.J.
,
Bolanos R.
,
Fasulo D.
,
Halldorsson B.V.
,
Hannenhalli S.
,
Turner R.
,
Yooseph S.
,
Lu F.
,
Nusskern D.R.
,
Shue B.C.
,
Zheng X.H.
,
Zhong F.
,
Delcher A.L.
,
Huson D.H.
,
Kravitz S.A.
,
Mouchard L.
,
Reinert K.
,
Remington K.A.
,
Clark A.G.
,
Waterman M.S.
,
Eichler E.E.
,
Adams M.D.
,
Hunkapiller M.W.
,
Myers E.W.
,
Venter J.C.
more...
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases
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Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
9.
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Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3)
tissue :
Brain .
10.
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Cited for : PROTEIN SEQUENCE OF 55-59;SUBCELLULAR LOCATION;PROTEOLYTIC CLEAVAGE
11.
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Cited for : NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 171-613
tissue :
Brain .
12.
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Cited for : REVIEW
13.
"Apoptosis-inducing factor (AIF) inhibits protein synthesis by interacting with the eukaryotic translation initiation factor 3 subunit p44 (eIF3g)."
Kim J.T.
,
Kim K.D.
,
Song E.Y.
,
Lee H.G.
,
Kim J.W.
,
Kim J.W.
,
Chae S.K.
,
Kim E.
,
Lee M.S.
,
Yang Y.
,
Lim J.S.
FEBS Lett.580:6375-6383(2006)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
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Cited for : FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH EIF3G
14.
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Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-105;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Cervix carcinoma .
15.
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Cited for : UBIQUITINATION BY XIAP/BIRC4;INTERACTION WITH XIAP/BIRC4
16.
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Cited for : PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-268;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue :
Cervix carcinoma .
17.
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Cited for : FUNCTION
18.
"A brain-specific isoform of mitochondrial apoptosis-inducing factor: AIF2."
Hangen E.
,
De Zio D.
,
Bordi M.
,
Zhu C.
,
Dessen P.
,
Caffin F.
,
Lachkar S.
,
Perfettini J.L.
,
Lazar V.
,
Benard J.
,
Fimia G.M.
,
Piacentini M.
,
Harper F.
,
Pierron G.
,
Vicencio J.M.
,
Benit P.
,
de Andrade A.
,
Hoglinger G.
,
Culmsee C.
,
Rustin P.
,
Blomgren K.
,
Cecconi F.
,
Kroemer G.
,
Modjtahedi N.
more...
Cell Death Differ.17:1155-1166(2010)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
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Cited for : ALTERNATIVE SPLICING (ISOFORM 3);SUBCELLULAR LOCATION (ISOFORM 3);SUBUNIT
19.
"Vital function of PRELI and essential requirement of its LEA motif."
McKeller M.R.
,
Herrera-Rodriguez S.
,
Ma W.
,
Ortiz-Quintero B.
,
Rangel R.
,
Cande C.
,
Sims-Mourtada J.C.
,
Melnikova V.
,
Kashi C.
,
Phan L.M.
,
Chen Z.
,
Huang P.
,
Dunner K. Jr.
,
Kroemer G.
,
Singh K.K.
,
Martinez-Valdez H.
Cell Death Dis.1:E21-E21(2010)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
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Cited for : INTERACTION WITH PRELID1
20.
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Cited for : IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
21.
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Cited for : UBIQUITINATION AT LYS-255 BY XIAP/BIRC4
22.
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Cited for : X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 121-613 IN COMPLEX WITH FAD;SUBCELLULAR LOCATION;DNA-BINDING
23.
"Cowchock syndrome is associated with a mutation in apoptosis-inducing factor."
Rinaldi C.
,
Grunseich C.
,
Sevrioukova I.F.
,
Schindler A.
,
Horkayne-Szakaly I.
,
Lamperti C.
,
Landoure G.
,
Kennerson M.L.
,
Burnett B.G.
,
Boennemann C.
,
Biesecker L.G.
,
Ghezzi D.
,
Zeviani M.
,
Fischbeck K.H.
Am. J. Hum. Genet.91:1095-1102(2012)
[
PubMed ]
[
Europe PMC ]
[
Abstract ]
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Cited for : X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 103-613 IN COMPLEX WITH FAD;FUNCTION;COFACTOR;BIOPHYSICOCHEMICAL PROPERTIES;DNA-BINDING;SUBUNIT;SUBCELLULAR LOCATION;TISSUE SPECIFICITY;VARIANT COWCK VAL-493;CHARACTERIZATION OF VARIANT COWCK VAL-493
24.
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Cited for : VARIANT COXPD6 ARG-201 DEL;CHARACTERIZATION OF VARIANT COXPD6 ARG-201 DEL;FUNCTION
25.
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Cited for : VARIANT COXPD6 GLU-308