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Index > Protein center > AIFM1(Gene name) > Human
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  • AIFM1 (Gene name),
  • Apoptosis-inducing factor 1, mitochondrial (Protein name ),  AIFM1_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    AIFM1(AIF;PDCD8);
    Protein name:
    Apoptosis-inducing factor 1, mitochondrial;
    Alternative:
    Programmed cell death protein 8;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Interacts with XIAP. Interacts (via N-terminus) with EIF3G (via C-terminus).
    Function:
    Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
    Subcellular Location:
    Mitochondrion intermembrane space Mitochondrion inner membrane Cytoplasm Nucleus Cytoplasm perinuclear region Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.
    Protein Attributes:
    Sequence length:
    613
    Sequence:
    50:
    MFRCGGLAAG | ALKQKLVPLV | RTVCVRSPRQ | RNRLPGNLFQ | RWHVPLELQM | 
    100:
    TRQMASSGAS | GGKIDNSVLV | LIVGLSTVGA | GAYAYKTMKE | DEKRYNERIS | 
    150:
    GLGLTPEQKQ | KKAALSASEG | EEVPQDKAPS | HVPFLLIGGG | TAAFAAARSI | 
    200:
    RARDPGARVL | IVSEDPELPY | MRPPLSKELW | FSDDPNVTKT | LRFKQWNGKE | 
    250:
    RSIYFQPPSF | YVSAQDLPHI | ENGGVAVLTG | KKVVQLDVRD | NMVKLNDGSQ | 
    300:
    ITYEKCLIAT | GGTPRSLSAI | DRAGAEVKSR | TTLFRKIGDF | RSLEKISREV | 
    350:
    KSITIIGGGF | LGSELACALG | RKARALGTEV | IQLFPEKGNM | GKILPEYLSN | 
    400:
    WTMEKVRREG | VKVMPNAIVQ | SVGVSSGKLL | IKLKDGRKVE | TDHIVAAVGL | 
    450:
    EPNVELAKTG | GLEIDSDFGG | FRVNAELQAR | SNIWVAGDAA | CFYDIKLGRR | 
    500:
    RVEHHDHAVV | SGRLAGENMT | GAAKPYWHQS | MFWSDLGPDV | GYEAIGLVDS | 
    550:
    SLPTVGVFAK | ATAQDNPKSA | TEQSGTGIRS | ESETESEASE | ITIPPSTPAV | 
    600:
    PQAPVQGEDY | GKGVIFYLRD | KVVVGIVLWN | IFNRMPIARK | IIKDGEQHED | 
    613:
    LNEVAKLFNI | HED
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    Pfam:
    String:
    KEGG:
    KEGG:
    MIM:
    UniGene:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Human Apoptosis-inducing factor 1, mitochondrial
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    ELISA Kit for Human Apoptosis-inducing factor 1, mitochondrial
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    ELISA Kit for Human Apoptosis-inducing factor 1, mitochondrial
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    CLIA Kit for Human Apoptosis-inducing factor 1, mitochondrial
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    U1064r
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    Range:
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    CLIA Kit for Human Apoptosis-inducing factor 1, mitochondrial
    Cat.:
    U1064h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Human Apoptosis-inducing factor 1, mitochondrial
    Cat.:
    U1064m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human Apoptosis-inducing factor 1, mitochondrial
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    P1064Rb-m
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    40ug/0.2ml
    Polyclonal Antibody for Human Apoptosis-inducing factor 1, mitochondrial
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    P1064Rb-r
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    Packing:
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    Polyclonal Antibody for Human Apoptosis-inducing factor 1, mitochondrial
    Cat.:
    P1064Rb-h
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    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Human Apoptosis-inducing factor 1, mitochondrial
    Monoclonal Antibody for Human Apoptosis-inducing factor 1, mitochondrial
    Monoclonal Antibody for Human Apoptosis-inducing factor 1, mitochondrial
    Protein for Human Apoptosis-inducing factor 1, mitochondrial
    Protein for Human Apoptosis-inducing factor 1, mitochondrial
    Protein for Human Apoptosis-inducing factor 1, mitochondrial

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    References
    1. 1.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
    2. 2.
      "AIFsh, a novel apoptosis-inducing factor (AIF) pro-apoptotic isoform with potential pathological relevance in human cancer."
      Delettre C. , Yuste V.J. , Moubarak R.S. , Bras M. , Lesbordes-Brion J.-C. , Petres S. , Bellalou J. , Susin S.A.
      J. Biol. Chem.281:6413-6427(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5);PROTEIN SEQUENCE OF N-TERMINUS;SUBCELLULAR LOCATION (ISOFORM 5);TISSUE SPECIFICITY
    3. 3.
      "Identification and characterization of AIFsh2, a mitochondrial apoptosis-inducing factor (AIF) isoform with NADH oxidase activity."
      Delettre C. , Yuste V.J. , Moubarak R.S. , Bras M. , Robert N. , Susin S.A.
      J. Biol. Chem.281:18507-18518(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 4 AND 6);ALTERNATIVE SPLICING
    4. 4.
      Rhodes S.
      Submitted (1999-04) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3)
    5. 5.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3)
      tissue: Kidney.
    6. 6.
      "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L. , Schick M. , Neubert P. , Schatten R. , Henze S. , Korn B.
      Submitted (2004-06) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
    7. 7.
      "The DNA sequence of the human X chromosome."
      Ross M.T. , Grafham D.V. , Coffey A.J. , Scherer S. , McLay K. , Muzny D. , Platzer M. , Howell G.R. , Burrows C. , Bird C.P. , Frankish A. , Lovell F.L. , Howe K.L. , Ashurst J.L. , Fulton R.S. , Sudbrak R. , Wen G. , Jones M.C. , more...
      Nature434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    8. 8.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    9. 9.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3)
      tissue: Brain.
    10. 10.
      "Export of mitochondrial AIF in response to proapoptotic stimuli depends on processing at the intermembrane space."
      Otera H. , Ohsakaya S. , Nagaura Z. , Ishihara N. , Mihara K.
      EMBO J.24:1375-1386(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 55-59;SUBCELLULAR LOCATION;PROTEOLYTIC CLEAVAGE
    11. 11.
      Mei G. , Yu W. , Gibbs R.A.
      Submitted (1999-02) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 171-613
      tissue: Brain.
    12. 12.
      "Apoptosis-inducing factor (AIF): a ubiquitous mitochondrial oxidoreductase involved in apoptosis."
      Daugas E. , Nochy D. , Ravagnan L. , Loeffler M. , Susin S.A. , Zamzami N. , Kroemer G.
      FEBS Lett.476:118-123(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
    13. 13.
      "Apoptosis-inducing factor (AIF) inhibits protein synthesis by interacting with the eukaryotic translation initiation factor 3 subunit p44 (eIF3g)."
      Kim J.T. , Kim K.D. , Song E.Y. , Lee H.G. , Kim J.W. , Kim J.W. , Chae S.K. , Kim E. , Lee M.S. , Yang Y. , Lim J.S.
      FEBS Lett.580:6375-6383(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH EIF3G
    14. 14.
      "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H. , Olsen J.V. , Bairlein M. , Gnad F. , Oppermann F.S. , Korner R. , Greff Z. , Keri G. , Stemmann O. , Mann M.
      Mol. Cell31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-105;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    15. 15.
      "Apoptosis-inducing factor is a target for ubiquitination through interaction with XIAP."
      Wilkinson J.C. , Wilkinson A.S. , Galban S. , Csomos R.A. , Duckett C.S.
      Mol. Cell. Biol.28:237-247(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION BY XIAP/BIRC4;INTERACTION WITH XIAP/BIRC4
    16. 16.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-268;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
      tissue: Cervix carcinoma.
    17. 17.
      "Apoptosis-inducing factor plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells."
      Son Y.O. , Jang Y.S. , Heo J.S. , Chung W.T. , Choi K.C. , Lee J.C.
      Apoptosis14:796-808(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    18. 18.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ALTERNATIVE SPLICING (ISOFORM 3);SUBCELLULAR LOCATION (ISOFORM 3);SUBUNIT
    19. 19.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PRELID1
    20. 20.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
    21. 21.
      "Nondegradative ubiquitination of apoptosis inducing factor (AIF) by X-linked inhibitor of apoptosis at a residue critical for AIF-mediated chromatin degradation."
      Lewis E.M. , Wilkinson A.S. , Davis N.Y. , Horita D.A. , Wilkinson J.C.
      Biochemistry50:11084-11096(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION AT LYS-255 BY XIAP/BIRC4
    22. 22.
      "DNA binding is required for the apoptogenic action of apoptosis inducing factor."
      Ye H. , Cande C. , Stephanou N.C. , Jiang S. , Gurbuxani S. , Larochette N. , Daugas E. , Garrido C. , Kroemer G. , Wu H.
      Nat. Struct. Biol.9:680-684(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 121-613 IN COMPLEX WITH FAD;SUBCELLULAR LOCATION;DNA-BINDING
    23. 23.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 103-613 IN COMPLEX WITH FAD;FUNCTION;COFACTOR;BIOPHYSICOCHEMICAL PROPERTIES;DNA-BINDING;SUBUNIT;SUBCELLULAR LOCATION;TISSUE SPECIFICITY;VARIANT COWCK VAL-493;CHARACTERIZATION OF VARIANT COWCK VAL-493
    24. 24.
      "Severe X-linked mitochondrial encephalomyopathy associated with a mutation in apoptosis-inducing factor."
      Ghezzi D. , Sevrioukova I. , Invernizzi F. , Lamperti C. , Mora M. , D'Adamo P. , Novara F. , Zuffardi O. , Uziel G. , Zeviani M.
      Am. J. Hum. Genet.86:639-649(2010) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT COXPD6 ARG-201 DEL;CHARACTERIZATION OF VARIANT COXPD6 ARG-201 DEL;FUNCTION
    25. 25.
      "Early prenatal ventriculomegaly due to an AIFM1 mutation identified by linkage analysis and whole exome sequencing."
      Berger I. , Ben-Neriah Z. , Dor-Wolman T. , Shaag A. , Saada A. , Zenvirt S. , Raas-Rothschild A. , Nadjari M. , Kaestner K.H. , Elpeleg O.
      Mol. Genet. Metab.104:517-520(2011) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT COXPD6 GLU-308
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