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Index > Protein center > Alb(Gene name) > Rat
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  • Alb (Gene name),
  • Serum albumin (Protein name ),  ALBU_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    Alb;
    Protein name:
    Serum albumin;
    Alternative:

    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    608
    Sequence:
    50:
    MKWVTFLLLL | FISGSAFSRG | VFRREAHKSE | IAHRFKDLGE | QHFKGLVLIA | 
    100:
    FSQYLQKCPY | EEHIKLVQEV | TDFAKTCVAD | ENAENCDKSI | HTLFGDKLCA | 
    150:
    IPKLRDNYGE | LADCCAKQEP | ERNECFLQHK | DDNPNLPPFQ | RPEAEAMCTS | 
    200:
    FQENPTSFLG | HYLHEVARRH | PYFYAPELLY | YAEKYNEVLT | QCCTESDKAA | 
    250:
    CLTPKLDAVK | EKALVAAVRQ | RMKCSSMQRF | GERAFKAWAV | ARMSQRFPNA | 
    300:
    EFAEITKLAT | DVTKINKECC | HGDLLECADD | RAELAKYMCE | NQATISSKLQ | 
    350:
    ACCDKPVLQK | SQCLAEIEHD | NIPADLPSIA | ADFVEDKEVC | KNYAEAKDVF | 
    400:
    LGTFLYEYSR | RHPDYSVSLL | LRLAKKYEAT | LEKCCAEGDP | PACYGTVLAE | 
    450:
    FQPLVEEPKN | LVKTNCELYE | KLGEYGFQNA | VLVRYTQKAP | QVSTPTLVEA | 
    500:
    ARNLGRVGTK | CCTLPEAQRL | PCVEDYLSAI | LNRLCVLHEK | TPVSEKVTKC | 
    550:
    CSGSLVERRP | CFSALTVDET | YVPKEFKAET | FTFHSDICTL | PDKEKQIKKQ | 
    600:
    TALAELVKHK | PKATEDQLKT | VMGDFAQFVD | KCCKAADKDN | CFATEGPNLV | 
    608:
    ARSKEALA
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    SMR:
    KEGG:
    String:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Rat Serum albumin
    MSDS:
    Please sign in first.
    ELISA Kit for Rat Serum albumin
    Cat.:
    E1028h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Serum albumin
    Cat.:
    E1028r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Serum albumin
    Cat.:
    E1028d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Rat Serum albumin
    Cat.:
    E1028c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Serum albumin
    Cat.:
    E1028p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Serum albumin
    Cat.:
    E1028m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Rat Serum albumin
    MSDS:
    Please sign in first.
    CLIA Kit for Rat Serum albumin
    MSDS:
    Please sign in first.
    CLIA Kit for Rat Serum albumin
    Cat.:
    U1028m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Serum albumin
    Cat.:
    U1028r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat Serum albumin
    MSDS:
    Please sign in first.
    CLIA Kit for Rat Serum albumin
    Cat.:
    U1028h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Serum albumin
    Cat.:
    U1028d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Serum albumin
    Cat.:
    U1028c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Serum albumin
    Cat.:
    U1028p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Rat Serum albumin
    Polyclonal Antibody for Rat Serum albumin
    Polyclonal Antibody for Rat Serum albumin
    Polyclonal Antibody for Rat Serum albumin
    Cat.:
    P1028Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Serum albumin
    Polyclonal Antibody for Rat Serum albumin
    Cat.:
    P1028Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Serum albumin
    Polyclonal Antibody for Rat Serum albumin
    Cat.:
    P1028Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Monoclonal Antibody for Rat Serum albumin
    Monoclonal Antibody for Rat Serum albumin
    Cat.:
    M1028m
    Price:
    Please sign in first.
    Packing:
    40ug
    Monoclonal Antibody for Rat Serum albumin
    Monoclonal Antibody for Rat Serum albumin
    Monoclonal Antibody for Rat Serum albumin
    Monoclonal Antibody for Rat Serum albumin
    Monoclonal Antibody for Rat Serum albumin
    Monoclonal Antibody for Rat Serum albumin
    Protein for Rat Serum albumin
    Protein for Rat Serum albumin
    Protein for Rat Serum albumin
    Protein for Rat Serum albumin
    Protein for Rat Serum albumin
    Protein for Rat Serum albumin
    Protein for Rat Serum albumin
    Protein for Rat Serum albumin

    R&D Technical Data
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    s

    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤8.2%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤10.7%

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of ALBU into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    100

    95-105

    Plasma

    98

    93-104

     

     

     

     

    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALBU and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    96-104%

    93-106%

    94-105%

    95-106%

    EDTA plasma(n=5)

    92-107%

    96-105%

    93-107%

    96-103%

    heparin plasma(n=5)

    95-106%

     

    97-103%

    97-103%

    94-105%

     

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    References
    1. 1.
      "Nucleotide sequence of cloned rat serum albumin messenger RNA."
      Sargent T.D. , Yang M. , Bonner J.
      Proc. Natl. Acad. Sci. U.S.A.78:243-246(1981) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANT LEU-262
      tissue: Ovary.
    3. 3.
      "Rat liver pre-proalbumin: complete amino acid sequence of the pre-piece. Analysis of the direct translation product of albumin messenger RNA."
      Strauss A.W. , Bennett C.D. , Donohue A.M. , Rodkey J.A. , Alberts A.W.
      J. Biol. Chem.252:6846-6855(1977) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 1-38;PROTEOLYTIC PROCESSING
    4. 4.
      "Amino acid sequences of fragments I and II obtained by cyanogen bromide cleavage of rat serum albumin."
      Isemura S. , Ikenaka T.
      J. Biochem.83:35-48(1978) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 25-222
    5. 5.
      "Structure of a biologically active neurotensin-related peptide obtained from pepsin-treated albumin(s)."
      Carraway R.E. , Mitra S.P. , Cochrane D.E.
      J. Biol. Chem.262:5968-5973(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 166-174
      tissue: Plasma.
    6. 6.
      Lubec G. , Afjehi-Sadat L. , Chen W.-Q. , Kang S.U.
      Submitted (2007-07) to the UniProtKB
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 35-57; 66-75; 89-97; 168-181; 247-264; 287-298; 361-372; 376-383; 414-434; 439-452; 470-483; 509-524; 528-545; 570-581 AND 585-602;IDENTIFICATION BY MASS SPECTROMETRY
      strain: Sprague-Dawley.
      tissue: Brain.
      tissue: Hippocampus.
      tissue: Spinal cord.
    7. 7.
      "Fragmentation of rat serum albumin by cyanogen bromide cleavage and the amino acid sequences of four fragments."
      Isemura S. , Ikenaka T.
      J. Biochem.79:1183-1196(1976) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 223-288 AND 572-608
    8. 8.
      "Copper(II)-binding ability of human alpha-fetoprotein."
      Aoyagi Y. , Ikenaka T. , Ichida F.
      Cancer Res.38:3483-3486(1978) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: COPPER-BINDING
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