AMELX ELISA Kit for Bovine(Bos taurus) | EIAab.comAMELX ELISA Kit for Bovine(Bos taurus)

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AMELX (Gene name),

Amelogenin, X isoform (Protein name ), AMELX_BOVIN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

AMELX;

Protein name:

Amelogenin, X isoform;

Alternative:

Class I amelogenin;

Organism:

Bovine (Bos taurus).

General Annotation

Sub Unit:

N/A

Function:

Plays a role in the biomineralization of teeth. Seems to regulate the formation of crystallites during the secretory stage of tooth enamel development. Thought to play a major role in the structural organization and mineralization of developing enamel.

Subcellular Location:

Secreted extracellular space extracellular matrix

Protein Attributes:

Sequence length:

213

Sequence:

50:

100:

150:

200:

213:

WPATDKTKRE | EVD

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Bt.28016

Pfam:

PF02948

String:

P02817

KEGG:

bta:281620

Uniprot:

P02817

FOR

ELISA Kit for Bovine Amelogenin, X isoform

Cat.:

E14172h

Price:

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Manual:

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MSDS:

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Packing:

96T

Range:

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ELISA Kit for Bovine Amelogenin, X isoform

Cat.:

E14172m

Price:

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

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ELISA Kit for Bovine Amelogenin, X isoform

Cat.:

E14172r

Price:

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Manual:

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Packing:

96T

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ELISA Kit for Bovine Amelogenin, X isoform

MSDS:

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CLIA Kit for Bovine Amelogenin, X isoform

MSDS:

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CLIA Kit for Bovine Amelogenin, X isoform

Cat.:

U14172r

Price:

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

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CLIA Kit for Bovine Amelogenin, X isoform

Cat.:

U14172h

Price:

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

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CLIA Kit for Bovine Amelogenin, X isoform

Cat.:

U14172m

Price:

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

Range:

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Polyclonal Antibody for Bovine Amelogenin, X isoform

Cat.:

P14172Rb-h

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Bovine Amelogenin, X isoform

Monoclonal Antibody for Bovine Amelogenin, X isoform

Protein for Bovine Amelogenin, X isoform

Cat.:

R14172h

Price:

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Packing:

1mg

Purity:

>90%

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.1%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.5%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.1%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of AMELX into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	104	97-108
Plasma	103	95-106

Recovery was determined by spiking various levels of AMELX into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	92-104
Plasma	99	93-107

Recovery was determined by spiking various levels of AMELX into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	95-103
Plasma	100	98-102

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AMELX and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-112%	97-109%	95-111%	99-107%
EDTA plasma(n=5)	99-105%	100-107%	97-109%	94-112%
heparin plasma(n=5)	95-110%	98-106%	96-111%	98-108%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	89-106%	92-102%	91-104%	94-102%
EDTA plasma(n=5)	91-107%	95-103%	92-107%	95-103%
heparin plasma(n=5)	93-104%	94-106%	96-104%	92-105%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-102%	93-105%	94-103%	97-102%
EDTA plasma(n=5)	94-106%	92-107%	96-104%	93-107%
heparin plasma(n=5)	95-104%	98-102%	95-103%	96-103%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Structure and expression of the bovine amelogenin gene."
Gibson C. , Golub E. , Herold R. , Risser M. , Ding W. , Shimokawa H. , Young M. , Termine J. , Rosenbloom J.
Biochemistry30:1075-1079(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"Complete amino acid sequence of amelogenin in developing bovine enamel."
Takagi T. , Suzuki M. , Baba T. , Minegishi K. , Sasaki S.
Biochem. Biophys. Res. Commun.121:592-597(1984) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PRELIMINARY PROTEIN SEQUENCE OF 17-201
3.

"Identification of the leucine-rich amelogenin peptide (LRAP) as the translation product of an alternatively spliced transcript."
Gibson C.W. , Golub E. , Ding W. , Shimokawa H. , Young M. , Termine J. , Rosenbloom J.
Biochem. Biophys. Res. Commun.174:1306-1312(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ALTERNATIVE SPLICING (ISOFORM 2)
tissue: Tooth.
4.

"Amelogenin post-translational modifications: carboxy-terminal processing and the phosphorylation of bovine and porcine 'TRAP' and 'LRAP' amelogenins."
Fincham A.G. , Moradian-Oldak J.
Biochem. Biophys. Res. Commun.197:248-255(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 17-49 AND 188-213;PHOSPHORYLATION AT SER-32
5.

"Secondary structure and limited three-dimensional structure of bovine amelogenin."
Renugopalakrishnan V. , Prabhakaran M. , Huang S.G. , Balasubramaniam A. , Strawich E. , Glimcher M.J.
Connect. Tissue Res.22:131-138(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR

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