Ang ELISA Kit for Mouse(Mus musculus) | EIAab.comAng ELISA Kit for Mouse(Mus musculus)

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Ang (Gene name),

Angiogenin (Protein name ), ANGI_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Ang(Ang1;Rnase5;Rnase5a);

Protein name:

Angiogenin;

Alternative:

Ribonuclease 5(RNase 5);Angiogenin-1;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Interacts with and forms a tight 1:1 complex with RNH1. Dimerization of two such complexes may occur.

Function:

May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

145

Sequence:

50:

100:

145:

VTTCKHTGGS | PRPPCQYRAS | AGFRHVVIAC | ENGLPVHFDE | SFFSL

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Mm.202665

STRING:

10090.ENSMUSP00000067434

String:

P21570

SMR:

P21570

KEGG:

mmu:11727

Pfam:

PF00074

Uniprot:

P21570

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ELISA Kit for Mouse Angiogenin

Cat.:

E0007h

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96T

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ELISA Kit for Mouse Angiogenin

Cat.:

E0007m

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96T

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ELISA Kit for Mouse Angiogenin

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ELISA Kit for Mouse Angiogenin

Cat.:

E0007p

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ELISA Kit for Mouse Angiogenin

Cat.:

E0007c

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96T

CLIA Kit for Mouse Angiogenin

Cat.:

U0007p

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96T

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CLIA Kit for Mouse Angiogenin

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CLIA Kit for Mouse Angiogenin

Cat.:

U0007h

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Looking for manual.

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96T

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CLIA Kit for Mouse Angiogenin

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U0007m

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CLIA Kit for Mouse Angiogenin

Cat.:

U0007c

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96T

Polyclonal Antibody for Mouse Angiogenin

Cat.:

P0007Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse Angiogenin

Cat.:

P0007Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse Angiogenin

Monoclonal Antibody for Mouse Angiogenin

Protein for Mouse Angiogenin

R&D Technical Data

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 5.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 9.2%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	95	91-101
Plasma	94	90-99

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	89-99%	91-102%	93-103%	90-104%
EDTA plasma(n=5)	89-98%	90-103%	91-97%	92-102%
heparin plasma(n=5)	90-101%	92-105%	94-107%	91-106%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Isolation and sequencing of mouse angiogenin DNA."
Bond M.D. , Vallee B.L.
Biochem. Biophys. Res. Commun.171:988-995(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
2.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: FVB/N.
tissue: Liver.
3.

"Characterization and sequencing of rabbit, pig and mouse angiogenins: discernment of functionally important residues and regions."
Bond M.D. , Strydom D.J. , Vallee B.L.
Biochim. Biophys. Acta1162:177-186(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL PROTEIN SEQUENCE
tissue: Serum.
4.

"Stress induces tRNA cleavage by angiogenin in mammalian cells."
Fu H. , Feng J. , Liu Q. , Sun F. , Tie Y. , Zhu J. , Xing R. , Sun Z. , Zheng X.
FEBS Lett.583:437-442(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
5.

"Structure of murine angiogenin: features of the substrate- and cell-binding regions and prospects for inhibitor-binding studies."
Holloway D.E. , Chavali G.B. , Hares M.C. , Subramanian V. , Acharya K.R.
Acta Crystallogr. D61:1568-1578(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 25-145;DISULFIDE BONDS

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