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Index > Protein center > ANXA5(Gene name) > Human
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  • ANXA5 (Gene name),
  • Annexin A5 (Protein name ),  ANXA5_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    ANXA5(ANX5;ENX2;PP4);
    Protein name:
    Annexin A5;
    Alternative:
    Vascular anticoagulant-alpha(VAC-alpha);Annexin V;Anchorin CII;Calphobindin I(CBP-I);Annexin-5;Lipocortin V;Endonexin II;Placental anticoagulant protein I(PAP-I);Placental anticoagulant protein 4(PP4);Thromboplastin inhibitor;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Monomer. Binds ATRX and EIF5B (By similarity). Interacts with hepatitis B virus (HBV).
    Function:
    This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
    Subcellular Location:
    N/A
    Protein Attributes:
    Sequence length:
    320
    Sequence:
    50:
    MAQVLRGTVT | DFPGFDERAD | AETLRKAMKG | LGTDEESILT | LLTSRSNAQR | 
    100:
    QEISAAFKTL | FGRDLLDDLK | SELTGKFEKL | IVALMKPSRL | YDAYELKHAL | 
    150:
    KGAGTNEKVL | TEIIASRTPE | ELRAIKQVYE | EEYGSSLEDD | VVGDTSGYYQ | 
    200:
    RMLVVLLQAN | RDPDAGIDEA | QVEQDAQALF | QAGELKWGTD | EEKFITIFGT | 
    250:
    RSVSHLRKVF | DKYMTISGFQ | IEETIDRETS | GNLEQLLLAV | VKSIRSIPAY | 
    300:
    LAETLYYAMK | GAGTDDHTLI | RVMVSRSEID | LFNIRKEFRK | NFATSLYSMI | 
    320:
    KGDTSGDYKK | ALLLLCGEDD | 
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    Pfam:
    String:
    MIM:
    KEGG:
    SMR:
    UniGene:
    Uniprot:
     
    FOR
    ELISA Kit for Human Annexin A5
    Cat.:
    E0259h
    Price:
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Human Annexin A5
    Cat.:
    E0259r
    Price:
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Human Annexin A5
    Cat.:
    E0259m
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    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human Annexin A5
    Cat.:
    E0259b
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Human Annexin A5
    Cat.:
    E0259c
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Annexin A5
    Cat.:
    U0259c
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Annexin A5
    Cat.:
    U0259b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Annexin A5
    Cat.:
    U0259h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human Annexin A5
    Cat.:
    U0259r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    CLIA Kit for Human Annexin A5
    Cat.:
    U0259m
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    MSDS:
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    Packing:
    96T
    Polyclonal Antibody for Human Annexin A5
    Cat.:
    P0259Rb-h
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Annexin A5
    Cat.:
    P0259Rb-m
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Annexin A5
    Cat.:
    P0259Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human Annexin A5
    Polyclonal Antibody for Human Annexin A5
    Monoclonal Antibody for Human Annexin A5
    Monoclonal Antibody for Human Annexin A5
    Monoclonal Antibody for Human Annexin A5
    Monoclonal Antibody for Human Annexin A5
    Monoclonal Antibody for Human Annexin A5
    Protein for Human Annexin A5
    Protein for Human Annexin A5
    Protein for Human Annexin A5
    Protein for Human Annexin A5
    Protein for Human Annexin A5

    R&D Technical Data
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

    s

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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤6.3%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤12.5%

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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of ANXA5 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    97

    95-101

    Plasma

    98

    93-102

     

     

     

     

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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ANXA5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-102%

    93-101%

    91-103%

    94-101%

    EDTA plasma(n=5)

    94-99%

    93-100%

    93-102%

    96-99%

    heparin plasma(n=5)

    95-100%

     

    92-103%

    95-101%

    95-102%

     

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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    EDTA plasma(n=5)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    References
    1. 1.
      "Primary structure of human placental anticoagulant protein."
      Funakoshi T. , Hendrickson L.E. , McMullen B.A. , Fujikawa K.
      Biochemistry26:8087-8092(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      "Structure and expression of cDNA for an inhibitor of blood coagulation isolated from human placenta: a new lipocortin-like protein."
      Iwasaki A. , Suda M. , Nakao H. , Nagoya T. , Saino Y. , Arai K. , Mizoguchi T. , Sato F. , Yoshizaki H. , Hirata M. , Miyata T. , Shidara Y. , Murata M. , Maki M.
      J. Biochem.102:1261-1273(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 2-320
    3. 3.
      "Cloning and expression of cDNA for human vascular anticoagulant, a Ca2+-dependent phospholipid-binding protein."
      Maurer-Fogy I. , Reutelingsperger C.P.M. , Pieters J. , Bodo G. , Stratowa C. , Hauptmann R.
      Eur. J. Biochem.174:585-592(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PARTIAL PROTEIN SEQUENCE
    4. 4.
      "Cloning and expression of cDNA for human endonexin II, a Ca2+ and phospholipid binding protein."
      Kaplan R. , Jaye M. , Burgess W.H. , Schlaepfer D.D. , Haigler H.T.
      J. Biol. Chem.263:8037-8043(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    5. 5.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    6. 6.
      "Characterization of cDNA encoding human placental anticoagulant protein (PP4): homology with the lipocortin family."
      Grundmann U. , Abel K.-J. , Bohn H. , Loebermann H. , Lottspeich F. , Kuepper H.
      Proc. Natl. Acad. Sci. U.S.A.85:3708-3712(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    7. 7.
      "The gene encoding human annexin V has a TATA-less promoter with a high G+C content."
      Fernandez M.-P. , Morgan R.O. , Fernandez M.R. , Carcedo M.-T.
      Gene149:253-260(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
      tissue: Lung.
    8. 8.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    9. 9.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Neuroblastoma.
    10. 10.
      "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Halleck A. , Ebert L. , Mkoundinya M. , Schick M. , Eisenstein S. , Neubert P. , Kstrang K. , Schatten R. , Shen B. , Henze S. , Mar W. , Korn B. , Zuo D. , Hu Y. , LaBaer J.
      Submitted (2004-06) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    11. 11.
      "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W. , Graves T.A. , Fulton R.S. , Fulton L.A. , Pepin K.H. , Minx P. , Wagner-McPherson C. , Layman D. , Wylie K. , Sekhon M. , Becker M.C. , Fewell G.A. , Delehaunty K.D. , Miner T.L. , Nash W.E. , Kremitzki C. , Oddy L. , Du H. , more...
      Nature434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    12. 12.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    13. 13.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Muscle.
      tissue: Ovary.
      tissue: Skin.
    14. 14.
      "A 32 kDa lipocortin from human mononuclear cells appears to be identical with the placental inhibitor of blood coagulation."
      Rothhut R. , Comera C. , Cortial S. , Haumont P.-Y. , Diep Le K.H. , Cavadore J.-C. , Conard J. , Russo-Marie F. , Lederer F.
      Biochem. J.263:929-935(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL PROTEIN SEQUENCE
    15. 15.
      Quadroni M. , Potts A. , Barblan J. , Bienvenut W.V.
      Submitted (2005-01) to the UniProtKB
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 7-18; 30-45; 187-201 AND 277-286;IDENTIFICATION BY MASS SPECTROMETRY
      tissue: Melanoma.
    16. 16.
      "Endonexin II, present on human liver plasma membranes, is a specific binding protein of small hepatitis B virus (HBV) envelope protein."
      Hertogs K. , Leenders W.P. , Depla E. , De Bruin W.C. , Meheus L. , Raymackers J. , Moshage H. , Yap S.H.
      Virology197:549-557(1993) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-31; 93-108; 176-188 AND 304-319;INTERACTION WITH HBV
    17. 17.
      "Structural and functional characterization of endonexin II, a calcium- and phospholipid-binding protein."
      Schlaepfer D.D. , Mehlman T. , Burgess W.H. , Haigler H.T.
      Proc. Natl. Acad. Sci. U.S.A.84:6078-6082(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 86-131; 259-297 AND 300-320
    18. 18.
      "Sedimentation equilibrium analysis of five lipocortin-related phospholipase A2 inhibitors from human placenta. Evidence against a mechanistically relevant association between enzyme and inhibitor."
      Ahn N.G. , Teller D.C. , Bienkowski M.J. , McMullen B.A. , Lipkin E.W. , de Haen C.
      J. Biol. Chem.263:18657-18663(1988) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 85-93
      tissue: Placenta.
    19. 19.
      "Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes."
      Aboulaich N. , Vainonen J.P. , Stralfors P. , Vener A.V.
      Biochem. J.383:237-248(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 152-161 AND 246-260
      tissue: Adipocyte.
    20. 20.
      "Substrate and functional diversity of lysine acetylation revealed by a proteomics survey."
      Kim S.C. , Sprung R. , Chen Y. , Xu Y. , Ball H. , Pei J. , Cheng T. , Kho Y. , Xiao H. , Xiao L. , Grishin N.V. , White M. , Yang X.-J. , Zhao Y.
      Mol. Cell23:607-618(2006) [PubMed] [Europe PMC] [Abstract]
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      Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
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      Science325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
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