Annexin A5 (Protein name
), ANXA5_MOUSE from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Gene name:
Anxa5(Anx5);
Protein name:
Annexin A5;
Alternative:
Vascular anticoagulant-alpha(VAC-alpha);Annexin V;Anchorin CII;Calphobindin I(CBP-I);Annexin-5;Lipocortin V;Endonexin II;Placental anticoagulant protein I(PAP-I);Placental anticoagulant protein 4(PP4);Thromboplastin inhibitor;
Organism:
Mouse (Mus musculus).
General Annotation
Sub Unit:
Monomer. Binds ATRX and EIF5B.
Function:
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
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s
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s
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤12.5%
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.7%
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Recovery
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Recovery was determined by spiking various levels of ANXA5 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
97
95-101
Plasma
98
93-102
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Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
91-103
Plasma
98
93-106
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ANXA5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
92-102%
93-101%
91-103%
94-101%
EDTA plasma(n=5)
94-99%
93-100%
93-102%
96-99%
heparin plasma(n=5)
95-100%
92-103%
95-101%
95-102%
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
92-105%
90-101%
95-105%
92-109%
EDTA plasma(n=5)
89-95%
93105%
89-94%
90-106%
heparin plasma(n=5)
93-104%
94-110%
101-111%
91-110%
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Cited for: PROTEIN SEQUENCE OF 28-43; 62-74; 116-124; 192-199; 226-240 AND 275-283;IDENTIFICATION BY MASS SPECTROMETRY
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Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-68 AND LYS-95;SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-288;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]