APAF1 ELISA Kit for Human(Homo sapiens) | EIAab.comAPAF1 ELISA Kit for Human(Homo sapiens)

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APAF1 (Gene name),

Apoptotic protease-activating factor 1 (Protein name ), APAF_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

APAF1(KIAA0413);

Protein name:

Apoptotic protease-activating factor 1(APAF-1);

Alternative:

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP.

Function:

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

Subcellular Location:

Cytoplasm

Protein Attributes:

Sequence length:

1248

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

750:

800:

850:

900:

950:

1000:

1050:

1100:

1150:

1200:

1248:

WWNVVTGESS | QTFYTNGTNL | KKIHVSPDFK | TYVTVDNLGI | LYILQTLE

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

O14727

KEGG:

hsa:317

UniGene:

Hs.728891

UniGene:

Hs.552567

SMR:

O14727

MIM:

602233

Pfam:

PF00619

Uniprot:

O14727

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ELISA Kit for Human APAF-1

Cat.:

E1054m

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96T

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ELISA Kit for Human APAF-1

Cat.:

E1054r

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96T

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ELISA Kit for Human APAF-1

Cat.:

E1054h

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96T

ELISA Kit for Human APAF-1

Cat.:

E1054d

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Packing:

96T

CLIA Kit for Human APAF-1

Cat.:

U1054m

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MSDS:

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Packing:

96T

CLIA Kit for Human APAF-1

Cat.:

U1054h

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Packing:

96T

CLIA Kit for Human APAF-1

Cat.:

U1054r

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Manual:

Looking for manual.

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Packing:

96T

Range:

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CLIA Kit for Human APAF-1

Cat.:

U1054d

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96T

Polyclonal Antibody for Human APAF-1

Cat.:

P1054Rb-r

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human APAF-1

Cat.:

P1054Rb-m

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40ug/0.2ml

Polyclonal Antibody for Human APAF-1

Cat.:

P1054Rb-h

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human APAF-1

Protein for Human APAF-1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3."
Zou H. , Henzel W.J. , Liu X. , Lutschg A. , Wang X.
Cell90:405-413(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3);PARTIAL PROTEIN SEQUENCE
tissue: Cervix carcinoma.
2.

"Three new types of Apaf-1 in mammalian cells."
Hahn C. , Hirsch B. , Jahnke D. , Duerkop H. , Stein H.
Biochem. Biophys. Res. Commun.261:746-749(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 4 AND 5)
tissue: Cervix carcinoma.
tissue: Heart.
tissue: Peripheral blood.
3.

"Cytochrome c and dATP-mediated oligomerization of Apaf-1 is a prerequisite for procaspase-9 activation."
Saleh A. , Srinivasula S.M. , Acharya S. , Fishel R. , Alnemri E.S.
J. Biol. Chem.274:17941-17945(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)
tissue: T-cell.
4.

"Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis."
Hu Y. , Benedict M.A. , Ding L. , Nunez G.
EMBO J.18:3586-3595(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);FUNCTION;SUBUNIT;MUTAGENESIS OF LYS-160 AND MET-368
tissue: Kidney.
5.

"APAF-1-ALT, a novel alternative splicing form of APAF-1, potentially causes impeded ability of undergoing DNA damage-induced apoptosis in the LNCaP human prostate cancer cell line."
Ogawa T. , Shiga K. , Hashimoto S. , Kobayashi T. , Horii A. , Furukawa T.
Biochem. Biophys. Res. Commun.306:537-543(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 6);FUNCTION;SUBCELLULAR LOCATION
tissue: Prostatic carcinoma.
6.

"Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro."
Ishikawa K. , Nagase T. , Nakajima D. , Seki N. , Ohira M. , Miyajima N. , Tanaka A. , Kotani H. , Nomura N. , Ohara O.
DNA Res.4:307-313(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
tissue: Brain.
7.

"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
Nakajima D. , Okazaki N. , Yamakawa H. , Kikuno R. , Ohara O. , Nagase T.
DNA Res.9:99-106(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SEQUENCE REVISION
8.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
9.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
10.

"The mammalian CED4 homologue, APAF1, exists as two distinct forms in human cells."
Roberts D.L. , Dalgleish R. , Cohen G.M. , MacFarlane M.
Submitted (1999-03) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 810-864 AND 866-883
11.

"Cloning of variant Apaf1."
Won M. , Lee J.-W. , Ohr H.-H. , Kim D.-U. , Chung K.-S. , Lee M. , Yoo H.-S.
Submitted (2000-03) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-138 (ISOFORMS 1/4/5)
12.

"Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization."
Srinivasula S.M. , Ahmad M. , Fernandes-Alnemri T. , Alnemri E.S.
Mol. Cell1:949-957(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: APAF-1-MEDIATED OLIGOMERIZATION
13.

"Apaf-1 is a transcriptional target for E2F and p53."
Moroni M.C. , Hickman E.S. , Denchi E.L. , Caprara G. , Colli E. , Cecconi F. , Mueller H. , Helin K.
Nat. Cell Biol.3:552-558(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INDUCTION BY E2F AND TP53
14.

"Induced inhibition of ischemic/hypoxic injury by APIP, a novel Apaf-1-interacting protein."
Cho D.-H. , Hong Y.-M. , Lee H.-J. , Woo H.-N. , Pyo J.-O. , Mak T.W. , Jung Y.-K.
J. Biol. Chem.279:39942-39950(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH APIP
15.

"Calcium blocks formation of apoptosome by preventing nucleotide exchange in Apaf-1."
Bao Q. , Lu W. , Rabinowitz J.D. , Shi Y.
Mol. Cell25:181-192(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INHIBITION BY CALCIUM
16.

"Integrity of ATP binding site is essential for effective inhibition of the intrinsic apoptosis pathway by NAIP."
Karimpour S. , Davoodi J. , Ghahremani M.H.
Biochem. Biophys. Res. Commun.407:158-162(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH NAIP/BIRC1
17.

"Crystal structure of Apaf-1 caspase recruitment domain: an alpha-helical Greek key fold for apoptotic signaling."
Vaughn D.E. , Rodriguez J. , Lazebnik Y. , Joshua-Tor L.
J. Mol. Biol.293:439-447(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS) OF 1-97
18.

"Solution structure and mutagenesis of the caspase recruitment domain (CARD) from Apaf-1."
Day C.L. , Dupont C. , Lackmann M. , Vaux D.L. , Hinds M.G.
Cell Death Differ.6:1125-1132(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 1-97

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