APLF ELISA Kit for Human(Homo sapiens) | EIAab.comAPLF ELISA Kit for Human(Homo sapiens)

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Index > Protein center > APLF(Gene name) > Human

APLF (Gene name),

Aprataxin and PNK-like factor (Protein name ), APLF_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

APLF(C2orf13;PALF;XIP1);

Protein name:

Aprataxin and PNK-like factor;

Alternative:

PNK and APTX-like FHA domain-containing protein;Apurinic-apyrimidinic endonuclease APLF;XRCC1-interacting protein 1;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Interacts with LIG4, PARP1, XRCC1, XRCC4 and XRCC5.

Function:

Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.

Subcellular Location:

Nucleus Cytoplasm cytosol Localizes to DNA damage sites. Accumulates at single-strand breaks and double-strand breaks via the PBZ-type zinc fingers.

Protein Attributes:

Sequence length:

511

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

511:

LKEAKRFMKR | K

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Hs.720369

SMR:

Q8IW19

STRING:

9606.ENSP00000307004

KEGG:

hsa:200558

MIM:

611035

String:

Q8IW19

Pfam:

PF10283

Uniprot:

Q8IW19

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ELISA Kit for Human Aprataxin and PNK-like factor

Cat.:

E3951m

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Packing:

96T

ELISA Kit for Human Aprataxin and PNK-like factor

Cat.:

E3951h

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Manual:

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Packing:

96T

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ELISA Kit for Human Aprataxin and PNK-like factor

Cat.:

E3951b

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Packing:

96T

CLIA Kit for Human Aprataxin and PNK-like factor

Cat.:

U3951h

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Human Aprataxin and PNK-like factor

Cat.:

U3951m

Price:

Please sign in first.

MSDS:

Please sign in first.

Packing:

96T

CLIA Kit for Human Aprataxin and PNK-like factor

Cat.:

U3951b

Price:

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human Aprataxin and PNK-like factor

Cat.:

P3951Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Aprataxin and PNK-like factor

Cat.:

P3951Rb-h

Price:

Please sign in first.

Packing:

40ug/0.2ml

Polyclonal Antibody for Human Aprataxin and PNK-like factor

Monoclonal Antibody for Human Aprataxin and PNK-like factor

Protein for Human Aprataxin and PNK-like factor

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Liver.
2.

"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W. , Graves T.A. , Fulton R.S. , Fulton L.A. , Pepin K.H. , Minx P. , Wagner-McPherson C. , Layman D. , Wylie K. , Sekhon M. , Becker M.C. , Fewell G.A. , Delehaunty K.D. , Miner T.L. , Nash W.E. , Kremitzki C. , Oddy L. , Du H. , more...
Nature434:724-731(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
3.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Testis.
4.

"A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses."
Kanno S. , Kuzuoka H. , Sasao S. , Hong Z. , Lan L. , Nakajima S. , Yasui A.
EMBO J.26:2094-2103(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;CATALYTIC ACTIVITY;SUBCELLULAR LOCATION;POLY-ADP-RIBOSYLATION;INTERACTION WITH LIG4; PARP1; XRCC4 AND XRCC5
5.

"Human Xip1 (C2orf13) is a novel regulator of cellular responses to DNA strand breaks."
Bekker-Jensen S. , Fugger K. , Danielsen J.R. , Gromova I. , Sehested M. , Celis J. , Bartek J. , Lukas J. , Mailand N.
J. Biol. Chem.282:19638-19643(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;INTERACTION WITH XRCC1;PHOSPHORYLATION AT SER-116;MUTAGENESIS OF SER-116
6.

"APLF (C2orf13) is a novel human protein involved in the cellular response to chromosomal DNA strand breaks."
Iles N. , Rulten S. , El-Khamisy S.F. , Caldecott K.W.
Mol. Cell. Biol.27:3793-3803(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH PARP1; XRCC1; XRCC4 AND XRCC5;SUBCELLULAR LOCATION;PHOSPHORYLATION
7.

"APLF (C2orf13) facilitates nonhomologous end-joining and undergoes ATM-dependent hyperphosphorylation following ionizing radiation."
Macrae C.J. , McCulloch R.D. , Ylanko J. , Durocher D. , Koch C.A.
DNA Repair7:292-302(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH XRCC4;PHOSPHORYLATION
8.

"APLF (C2orf13) is a novel component of poly(ADP-ribose) signaling in mammalian cells."
Rulten S.L. , Cortes-Ledesma F. , Guo L. , Iles N.J. , Caldecott K.W.
Mol. Cell. Biol.28:4620-4628(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;ADP-RIBOSE-BINDING
9.

"Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins."
Ahel I. , Ahel D. , Matsusaka T. , Clark A.J. , Pines J. , Boulton S.J. , West S.C.
Nature451:81-85(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;DOMAIN PBZ-TYPE;POLY-ADP-RIBOSYLATION;ADP-RIBOSE-BINDING;MUTAGENESIS OF ARG-376; CYS-379; CYS-385; CYS-421 AND CYS-427
10.

"Solution structures of the two PBZ domains from human APLF and their interaction with poly(ADP-ribose)."
Eustermann S. , Brockmann C. , Mehrotra P.V. , Yang J.C. , Loakes D. , West S.C. , Ahel I. , Neuhaus D.
Nat. Struct. Mol. Biol.17:241-243(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 368-451 ALONE AND IN COMPLEX WITH ADP-RIBOSE ANALOG;POLY-ADP-RIBOSE BINDING SITES
11.

"Structure and identification of ADP-ribose recognition motifs of APLF and role in the DNA damage response."
Li G.Y. , McCulloch R.D. , Fenton A.L. , Cheung M. , Meng L. , Ikura M. , Koch C.A.
Proc. Natl. Acad. Sci. U.S.A.107:9129-9134(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 360-448;LINKER REGION;POLY-ADP-RIBOSE BINDING SITES

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