B4GALT5 ELISA Kit for Human(Homo sapiens) | EIAab.comB4GALT5 ELISA Kit for Human(Homo sapiens) | EIAab.com

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B4GALT5 (Gene name),

Beta-1,4-galactosyltransferase 5 (Protein name ), B4GT5_HUMAN from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

B4GALT5;

Protein name:

Beta-1,4-galactosyltransferase 5(Beta-1,4-GalTase 5;Beta4Gal-T5;b4Gal-T5);

Alternative:

UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5;Beta-1,4-GalT II;UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

N/A

Function:

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.

Subcellular Location:

Golgi apparatus Golgi stack membrane Single-pass type II membrane protein Trans cisternae of Golgi stack.

Protein Attributes:

Sequence length:

388

Sequence:

50:

MRARRGLLRL | PRRSLLAALF | FFSLSSSLLY | FVYVAPGIVN | TYLFMMQAQG |

100:

ILIRDNVRTI | GAQVYEQVLR | SAYAKRNSSV | NDSDYPLDLN | HSETFLQTTT |

150:

FLPEDFTYFA | NHTCPERLPS | MKGPIDINMS | EIGMDYIHEL | FSKDPTIKLG |

200:

GHWKPSDCMP | RWKVAILIPF | RNRHEHLPVL | FRHLLPMLQR | QRLQFAFYVV |

250:

EQVGTQPFNR | AMLFNVGFQE | AMKDLDWDCL | IFHDVDHIPE | SDRNYYGCGQ |

300:

MPRHFATKLD | KYMYLLPYTE | FFGGVSGLTV | EQFRKINGFP | NAFWGWGGED |

350:

DDLWNRVQNA | GYSVSRPEGD | TGKYKSIPHH | HRGEVQFLGR | YALLRKSKER |

388:

QGLDGLNNLN | YFANITYDAL | YKNITVNLTP | ELAQVNEY

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

UniGene:

String:

STRING:

9606.ENSP00000360776

MIM:

Pfam:

SMR:

Uniprot:

FOR

ELISA Kit for Human Beta-1,4-GalTase 5

Cat.:

E2493h

Price:

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MSDS:

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Packing:

96T

Range:

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ELISA Kit for Human Beta-1,4-GalTase 5

Cat.:

E2493m

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Manual:

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MSDS:

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Packing:

96T

Range:

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CLIA Kit for Human Beta-1,4-GalTase 5

Cat.:

U2493h

Price:

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Manual:

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MSDS:

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Packing:

96T

Range:

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CLIA Kit for Human Beta-1,4-GalTase 5

Cat.:

U2493m

Price:

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human Beta-1,4-GalTase 5

Cat.:

P2493Rb-h

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human Beta-1,4-GalTase 5

Cat.:

P2493Rb-m

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human Beta-1,4-GalTase 5

Monoclonal Antibody for Human Beta-1,4-GalTase 5

Protein for Human Beta-1,4-GalTase 5

Protein for Human Beta-1,4-GalTase 5

R&D Technical Data

s

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

s

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of B4GT5 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	100	92-105
Plasma	99	95-103

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of B4GT5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-109%	95-105%	97-104%	91-107%
Saliva(n=3)	94-106%	93-104%	92-106%	95-103%
heparin plasma(n=5)	91-108%	97-103%	96-104%	90-107%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
Saliva(n=3)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Molecular cloning of a human cDNA encoding beta-1,4-galactosyltransferase with 37% identity to mammalian UDP-Gal:GlcNAc beta-1,4-galactosyltransferase."
Sato T. , Furukawa K. , Bakker H. , van den Eijnden D.H. , van Die I.
Proc. Natl. Acad. Sci. U.S.A.95:472-477(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION;TISSUE SPECIFICITY
tissue: Mammary tumor.
2.

"The expanding beta 4-galactosyltransferase gene family: messages from the databanks."
Lo N.-W. , Shaper J.H. , Pevsner J. , Shaper N.L.
Glycobiology8:517-526(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P. , Matthews L.H. , Ashurst J.L. , Burton J. , Gilbert J.G.R. , Jones M. , Stavrides G. , Almeida J.P. , Babbage A.K. , Bagguley C.L. , Bailey J. , Barlow K.F. , Bates K.N. , Beard L.M. , Beare D.M. , Beasley O.P. , Bird C.P. , Blakey S.E. , more...
Nature414:865-871(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
4.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Cerebellum.
tissue: Lung.
6.

"Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions."
Amado M. , Almeida R. , Schwientek T. , Clausen H.
Biochim. Biophys. Acta1473:35-53(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW

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