COL4A3BP ELISA Kit for Human(Homo sapiens) | EIAab.comCOL4A3BP ELISA Kit for Human(Homo sapiens)

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COL4A3BP (Gene name),

Collagen type IV alpha-3-binding protein (Protein name ), C43BP_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

COL4A3BP(CERT;STARD11);

Protein name:

Collagen type IV alpha-3-binding protein;

Alternative:

Goodpasture antigen-binding protein(GPBP);Ceramide transfer protein(hCERT);StAR-related lipid transfer protein 11;START domain-containing protein 11(StARD11);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Interacts with COL4A3. Interacts with VAPA and VAPB. Interaction with VAPB is less efficient than with VAPA.

Function:

May mediate the intracellular trafficking of ceramide in a non-vesicular manner.

Subcellular Location:

Cytoplasm Golgi apparatus Endoplasmic reticulum Preferentially localized to the Golgi apparatus.

Protein Attributes:

Sequence length:

624

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

624:

EYPKFLKRFT | SYVQEKTAGK | PILF

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Hs.270437

String:

Q9Y5P4

Pfam:

PF00169

SMR:

Q9Y5P4

KEGG:

hsa:10087

MIM:

604677

STRING:

9606.ENSP00000369862

Uniprot:

Q9Y5P4

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ELISA Kit for Human Collagen type IV alpha-3-binding protein

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ELISA Kit for Human Collagen type IV alpha-3-binding protein

Cat.:

E14005m

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Packing:

96T

ELISA Kit for Human Collagen type IV alpha-3-binding protein

Cat.:

E14005h

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Manual:

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96T

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CLIA Kit for Human Collagen type IV alpha-3-binding protein

Cat.:

U14005h

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Manual:

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Packing:

96T

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CLIA Kit for Human Collagen type IV alpha-3-binding protein

Cat.:

U14005m

Price:

Please sign in first.

MSDS:

Please sign in first.

Packing:

96T

CLIA Kit for Human Collagen type IV alpha-3-binding protein

MSDS:

Please sign in first.

Polyclonal Antibody for Human Collagen type IV alpha-3-binding protein

Monoclonal Antibody for Human Collagen type IV alpha-3-binding protein

Protein for Human Collagen type IV alpha-3-binding protein

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.4%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of COL4A3BP into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	94-105
Plasma	100	96-107

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COL4A3BP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-103%	90-108%	95-105%	92-107%
EDTA plasma(n=5)	93-106%	92-107%	94-108%	91-110%
heparin plasma(n=5)	95-105%	96-104%	97-105%	93-109%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Characterization of a novel type of serine/threonine kinase that specifically phosphorylates the human goodpasture antigen."
Raya A. , Revert F. , Navarro S. , Saus J.
J. Biol. Chem.274:12642-12649(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);CHARACTERIZATION
2.

"Goodpasture antigen-binding protein, the kinase that phosphorylates the Goodpasture antigen, is an alternatively spliced variant implicated in autoimmune pathogenesis."
Raya A. , Revert-Ros F. , Martinez-Martinez P. , Navarro S. , Rosello E. , Vieites B. , Granero F. , Forteza J. , Saus J.
J. Biol. Chem.275:40392-40399(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2)
3.

"Molecular machinery for non-vesicular trafficking of ceramide."
Hanada K. , Kumagai K. , Yasuda S. , Miura Y. , Kawano M. , Fukasawa M. , Nishijima M.
Nature426:803-809(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2);FUNCTION;DOMAIN;SUBCELLULAR LOCATION
4.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3)
tissue: Lung.
tissue: Synovium.
5.

"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J. , Martin J. , Terry A. , Couronne O. , Grimwood J. , Lowry S. , Gordon L.A. , Scott D. , Xie G. , Huang W. , Hellsten U. , Tran-Gyamfi M. , She X. , Prabhakar S. , Aerts A. , Altherr M. , Bajorek E. , Black S. , more...
Nature431:268-274(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
7.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
tissue: Placenta.
8.

"Homo sapiens genomic sequence, containing DINB1 and GPBP gene."
Ogi T. , Yamamoto Y. , Ohmori H.
Submitted (2000-01) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-77
9.

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
10.

"Efficient trafficking of ceramide from the endoplasmic reticulum to the Golgi apparatus requires a VAMP-associated protein-interacting FFAT motif of CERT."
Kawano M. , Kumagai K. , Nishijima M. , Hanada K.
J. Biol. Chem.281:30279-30288(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH VAPA AND VAPB;SUBCELLULAR LOCATION;MUTAGENESIS OF ASP-324
11.

"Regulation of secretory transport by protein kinase D-mediated phosphorylation of the ceramide transfer protein."
Fugmann T. , Hausser A. , Schoeffler P. , Schmid S. , Pfizenmaier K. , Olayioye M.A.
J. Cell Biol.178:15-22(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-132;FUNCTION;MUTAGENESIS OF SER-132
12.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
13.

"Casein kinase I{gamma}2 down-regulates trafficking of ceramide in the synthesis of sphingomyelin."
Tomishige N. , Kumagai K. , Kusuda J. , Nishijima M. , Hanada K.
Mol. Biol. Cell20:348-357(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION BY CSNK1G2/CK1
14.

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Leukemic T-cell.
15.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
16.

"Structural basis for specific lipid recognition by CERT responsible for nonvesicular trafficking of ceramide."
Kudo N. , Kumagai K. , Tomishige N. , Yamaji T. , Wakatsuki S. , Nishijima M. , Hanada K. , Kato R.
Proc. Natl. Acad. Sci. U.S.A.105:488-493(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF 347-624 IN COMPLEXES WITH CERAMIDES AND DIACYLGLYCEROL;MUTAGENESIS OF GLU-472; GLN-493 AND ASN-530;FUNCTION;SUBCELLULAR LOCATION
17.

"Crystal structures of the CERT START domain with inhibitors provide insights into the mechanism of ceramide transfer."
Kudo N. , Kumagai K. , Matsubara R. , Kobayashi S. , Hanada K. , Wakatsuki S. , Kato R.
J. Mol. Biol.396:245-251(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.66 ANGSTROMS) OF 347-624 IN COMPLEXES WITH CERAMIDE AND INHIBITOR;MUTAGENESIS OF TRP-499;FUNCTION
18.

"Diagnostic exome sequencing in persons with severe intellectual disability."
de Ligt J. , Willemsen M.H. , van Bon B.W. , Kleefstra T. , Yntema H.G. , Kroes T. , Vulto-van Silfhout A.T. , Koolen D.A. , de Vries P. , Gilissen C. , del Rosario M. , Hoischen A. , Scheffer H. , de Vries B.B. , Brunner H.G. , Veltman J.A. , Vissers L.E.
N. Engl. J. Med.367:1921-1929(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT CYS-138

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