CBLB ELISA Kit for Human(Homo sapiens) | EIAab.comCBLB ELISA Kit for Human(Homo sapiens)

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CBLB (Gene name),

E3 ubiquitin-protein ligase CBL-B (Protein name ), CBLB_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

CBLB(Nbla00127;RNF56);

Protein name:

E3 ubiquitin-protein ligase CBL-B;

Alternative:

RING finger protein 56;Casitas B-lineage lymphoma proto-oncogene b;Signal transduction protein CBL-B;SH3-binding protein CBL-B;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Interacts with SH3 domain-containing proteins LCK, CRK and SORBS1. Interacts with LCP2 and ZAP70. May interact with CBL (By similarity). Interacts with SH3 domain-containing proteins VAV1, FYN, FGR, PLCG1, GRB2, CRKL, PIK3R1 and SH3KBP1/CIN85. Identified in heterotrimeric complexes with SH3KBP1/CIN85, CD2AP and ARHGEF7, where one CBLB peptide binds two copies of the other protein. Interacts with poly-ubiquitinated proteins. Dimerization is required for the binding of poly-ubiquitin, but not for the binding of mono-ubiquitin.

Function:

E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization.

Subcellular Location:

Cytoplasm Upon EGF stimulation, associates with endocytic vesicles.

Protein Attributes:

Sequence length:

982

Sequence:

50:

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250:

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350:

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450:

500:

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600:

650:

700:

750:

800:

850:

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950:

982:

RALEIAQNNV | EVARSILREF | AFPPPVSPRL | NL

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Hs.430589

String:

Q13191

STRING:

9606.ENSP00000264122

MIM:

604491

SMR:

Q13191

KEGG:

hsa:868

Pfam:

PF02262

Uniprot:

Q13191

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ELISA Kit for Human E3 ubiquitin-protein ligase CBL-B

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E2531h

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96T

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ELISA Kit for Human E3 ubiquitin-protein ligase CBL-B

Cat.:

E2531m

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96T

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ELISA Kit for Human E3 ubiquitin-protein ligase CBL-B

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E2531r

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96T

CLIA Kit for Human E3 ubiquitin-protein ligase CBL-B

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U2531m

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96T

CLIA Kit for Human E3 ubiquitin-protein ligase CBL-B

Cat.:

U2531h

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Packing:

96T

CLIA Kit for Human E3 ubiquitin-protein ligase CBL-B

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U2531r

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96T

Polyclonal Antibody for Human E3 ubiquitin-protein ligase CBL-B

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P2531Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human E3 ubiquitin-protein ligase CBL-B

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P2531Rb-m

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Polyclonal Antibody for Human E3 ubiquitin-protein ligase CBL-B

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P2531Rb-h

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Monoclonal Antibody for Human E3 ubiquitin-protein ligase CBL-B

Protein for Human E3 ubiquitin-protein ligase CBL-B

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Cloning and characterization of cbl-b: a SH3 binding protein with homology to the c-cbl proto-oncogene."
Keane M.M. , Rivero-Lezcano O.M. , Mitchell J.A. , Robbins K.C. , Lipkowitz S.
Oncogene10:2367-2377(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG; TRUNCATED 1 AND TRUNCATED 2)
2.

"Cbl-b, a member of the Sli-1/c-Cbl protein family, inhibits Vav-mediated c-Jun N-terminal kinase activation."
Bustelo X.R. , Crespo P. , Lopez-Barahona M. , Gutkind J.S. , Barbacid M.
Oncogene15:2511-2520(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG);TISSUE SPECIFICITY;INTERACTION WITH VAV1
3.

"Cbl-b in Taiwan population."
Shen C.-R. , Chen Y.-J. , Pai L.-M. , Liu C.-L.
Submitted (2005-12) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG)
4.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG)
tissue: Trachea.
5.

"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M. , Scherer S.E. , Kaul R. , Wang J. , Yu J. , Sudbrak R. , Buhay C.J. , Chen R. , Cree A. , Ding Y. , Dugan-Rocha S. , Gill R. , Gunaratne P. , Harris R.A. , Hawes A.C. , Hernandez J. , Hodgson A.V. , Hume J. , more...
Nature440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
7.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG);VARIANT LYS-584
tissue: Testis.
8.

"Neuroblastoma oligo-capping cDNA project: toward the understanding of the genesis and biology of neuroblastoma."
Ohira M. , Morohashi A. , Nakamura Y. , Isogai E. , Furuya K. , Hamano S. , Machida T. , Aoyama M. , Fukumura M. , Miyazaki K. , Suzuki Y. , Sugano S. , Hirato J. , Nakagawara A.
Cancer Lett.197:63-68(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 543-982 (ISOFORM LONG)
tissue: Neuroblastoma.
9.

"The full-ORF clone resource of the German cDNA consortium."
Bechtel S. , Rosenfelder H. , Duda A. , Schmidt C.P. , Ernst U. , Wellenreuther R. , Mehrle A. , Schuster C. , Bahr A. , Bloecker H. , Heubner D. , Hoerlein A. , Michel G. , Wedler H. , Koehrer K. , Ottenwaelder B. , Poustka A. , Wiemann S. , more...
BMC Genomics8:399-399(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 857-982 (ISOFORM LONG)
tissue: Small intestine.
10.

"Tyrosine phosphorylation and complex formation of Cbl-b upon T cell receptor stimulation."
Elly C. , Witte S. , Zhang Z. , Rosnet O. , Lipkowitz S. , Altman A. , Liu Y.-C.
Oncogene18:1147-1156(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY;MUTAGENESIS OF GLY-298; TYR-665 AND TYR-709;INTERACTION WITH GRB2 AND CRKL;PHOSPHORYLATION AT TYR-665 AND TYR-709;FUNCTION
11.

"cbl-b inhibits epidermal growth factor receptor signaling."
Ettenberg S.A. , Keane M.M. , Nau M.M. , Frankel M. , Wang L.-M. , Pierce J.H. , Lipkowitz S.
Oncogene18:1855-1866(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;PHOSPHORYLATION;INTERACTION WITH GRB2 AND PIK3R1
12.

"Cbl-b, a RING-type E3 ubiquitin ligase, targets phosphatidylinositol 3-kinase for ubiquitination in T cells."
Fang D. , Wang H.-Y. , Fang N. , Altman Y. , Elly C. , Liu Y.-C.
J. Biol. Chem.276:4872-4878(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH PIK3R1;MUTAGENESIS OF GLY-298 AND CYS-373
13.

"Cbl-b-dependent coordinated degradation of the epidermal growth factor receptor signaling complex."
Ettenberg S.A. , Magnifico A. , Cuello M. , Nau M.M. , Rubinstein Y.R. , Yarden Y. , Weissman A.M. , Lipkowitz S.
J. Biol. Chem.276:27677-27684(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION;MUTAGENESIS OF CYS-373
14.

"Proteolysis-independent regulation of PI3K by Cbl-b-mediated ubiquitination in T cells."
Fang D. , Liu Y.-C.
Nat. Immunol.2:870-875(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
15.

"CIN85 participates in Cbl-b-mediated down-regulation of receptor tyrosine kinases."
Szymkiewicz I. , Kowanetz K. , Soubeyran P. , Dinarina A. , Lipkowitz S. , Dikic I.
J. Biol. Chem.277:39666-39672(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SH3KBP1;SUBCELLULAR LOCATION
16.

"Cbl-c suppresses v-Src-induced transformation through ubiquitin-dependent protein degradation."
Kim M. , Tezuka T. , Tanaka K. , Yamamoto T.
Oncogene23:1645-1655(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
17.

"Cbl-b interacts with ubiquitinated proteins; differential functions of the UBA domains of c-Cbl and Cbl-b."
Davies G.C. , Ettenberg S.A. , Coats A.O. , Mussante M. , Ravichandran S. , Collins J. , Nau M.M. , Lipkowitz S.
Oncogene23:7104-7115(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH UBIQUITINATED PROTEINS;MUTAGENESIS OF 943-GLY-TYR-944 AND LEU-967
18.

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
19.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-521; SER-525 AND SER-529;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
20.

"PKC-theta modulates the strength of T cell responses by targeting Cbl-b for ubiquitination and degradation."
Gruber T. , Hermann-Kleiter N. , Hinterleitner R. , Fresser F. , Schneider R. , Gastl G. , Penninger J.M. , Baier G.
Sci. Signal.2:RA30-RA30(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-282
21.

"The N terminus of Cbl-c regulates ubiquitin ligase activity by modulating affinity for the ubiquitin-conjugating enzyme."
Ryan P.E. , Sivadasan-Nair N. , Nau M.M. , Nicholas S. , Lipkowitz S.
J. Biol. Chem.285:23687-23698(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION AS E3 UBIQUITIN-PROTEIN LIGASE;PHOSPHORYLATION AT TYR-363;MUTAGENESIS OF TYR-363
22.

"Cbl promotes clustering of endocytic adaptor proteins."
Jozic D. , Cardenes N. , Deribe Y.L. , Moncalian G. , Hoeller D. , Groemping Y. , Dikic I. , Rittinger K. , Bravo J.
Nat. Struct. Mol. Biol.12:972-979(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 899-914 IN COMPLEXES WITH SH3KBP1 AND ARHGEF7;MUTAGENESIS OF ARG-904 AND ARG-911;SUBUNIT
23.

"Atypical polyproline recognition by the CMS N-terminal Src homology 3 domain."
Moncalian G. , Cardenes N. , Deribe Y.L. , Spinola-Amilibia M. , Dikic I. , Bravo J.
J. Biol. Chem.281:38845-38853(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 902-912 IN COMPLEX WITH CD2AP;MUTAGENESIS OF ARG-904; LYS-907 AND ARG-911;SUBUNIT
24.

"Structural basis for ubiquitin-mediated dimerization and activation of the ubiquitin protein ligase Cbl-b."
Peschard P. , Kozlov G. , Lin T. , Mirza I.A. , Berghuis A.M. , Lipkowitz S. , Park M. , Gehring K.
Mol. Cell27:474-485(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.56 ANGSTROMS) OF 924-973 IN COMPLEX WITH UBIQUITIN;STRUCTURE BY NMR OF 924-973;TYROSINE PHOSPHORYLATION;MUTAGENESIS OF ALA-937; MET-940; PHE-946 AND ILE-966
25.

"Solution structure of RSGI RUH-065, a UBA domain from human cDNA."
RIKEN structural genomics initiative (RSGI)
Submitted (2007-05) to the PDB data bank

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 931-970
26.

"Crystal structure of Cbl-b TKB domain in complex with EGFR pY1069 peptide."
Structural genomics consortium (SGC)
Submitted (2010-10) to the PDB data bank

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.27 ANGSTROMS) OF 38-344 IN COMPLEX WITH PHOSPHO-EGFR PEPTIDE;INTERACTION WITH EGFR;HETERODIMER

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