CCL3 ELISA Kit for Human(Homo sapiens) | EIAab.comCCL3 ELISA Kit for Human(Homo sapiens)

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Index > Protein center > CCL3(Gene name) > Human

CCL3 (Gene name),

C-C motif chemokine 3 (Protein name ), CCL3_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

CCL3(G0S19-1;MIP1A;SCYA3);

Protein name:

C-C motif chemokine 3;

Alternative:

Macrophage inflammatory protein 1-alpha(MIP-1-alpha);G0/G1 switch regulatory protein 19-1;SIS-beta;PAT 464.1;Tonsillar lymphocyte LD78 alpha protein;Small-inducible cytokine A3;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Self-associates. Also heterodimer of MIP-1-alpha(4-69) and MIP-1-beta(3-69).

Function:

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

Sequence:

50:

92:

FETSSQCSKP | GVIFLTKRSR | QVCADPSEEW | VQKYVSDLEL | SA

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF00048

KEGG:

hsa:6348

String:

P10147

UniGene:

Hs.514107

SMR:

P10147

STRING:

9606.ENSP00000225245

MIM:

182283

Uniprot:

P10147

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ELISA Kit for Human C-C motif chemokine 3

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E0092h

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96T

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ELISA Kit for Human C-C motif chemokine 3

Cat.:

E0092r

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96T

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ELISA Kit for Human C-C motif chemokine 3

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E0092m

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ELISA Kit for Human C-C motif chemokine 3

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E0092b

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96T

ELISA Kit for Human C-C motif chemokine 3

Cat.:

E0092d

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96T

CLIA Kit for Human C-C motif chemokine 3

Cat.:

U0092m

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96T

CLIA Kit for Human C-C motif chemokine 3

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U0092b

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96T

CLIA Kit for Human C-C motif chemokine 3

Cat.:

U0092d

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Packing:

96T

CLIA Kit for Human C-C motif chemokine 3

Cat.:

U0092r

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Manual:

Looking for manual.

MSDS:

Please sign in first.

Packing:

96T

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CLIA Kit for Human C-C motif chemokine 3

Cat.:

U0092h

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Looking for manual.

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96T

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Polyclonal Antibody for Human C-C motif chemokine 3

Cat.:

P0092Rb-m

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40ug/0.2ml

Polyclonal Antibody for Human C-C motif chemokine 3

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P0092Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human C-C motif chemokine 3

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P0092Rb-h

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40ug/0.2ml

Polyclonal Antibody for Human C-C motif chemokine 3

Monoclonal Antibody for Human C-C motif chemokine 3

Protein for Human C-C motif chemokine 3

R&D Technical Data

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 5.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 7.8%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	93-108
Plasma	99	94-107

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-99%	91-102%	95-103%	96-107%
EDTA plasma(n=5)	91-97%	95-101%	94-102%	93-106%
heparin plasma(n=5)	93-104%	94-107%	101-108%	95-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"A cDNA clone used to study mRNA inducible in human tonsillar lymphocytes by a tumor promoter."
Obaru K. , Fukuda M. , Maeda S. , Shimada K.
J. Biochem.99:885-894(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]
tissue: Lymphocyte.
2.

"Mitogenic activation of human T cells induces two closely related genes which share structural similarities with a new family of secreted factors."
Zipfel P.F. , Balke J. , Irving S.G. , Kelly K. , Siebenlist U.
J. Immunol.142:1582-1590(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: T-cell.
3.

"Three human homologs of a murine gene encoding an inhibitor of stem cell proliferation."
Blum S. , Forsdyke R.E. , Forsdyke D.R.
DNA Cell Biol.9:589-602(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]
tissue: Lymphocyte.
4.

"Structures of human genes coding for cytokine LD78 and their expression."
Nakao M. , Nomiyama H. , Shimada K.
Mol. Cell. Biol.10:3646-3658(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
tissue: Lymphocyte.
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Natural killer cell.
6.

Jang J.S. , Kim B.E.
Submitted (1998-01) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 23-92
tissue: Leukocyte.
7.

"BB-10010: an active variant of human macrophage inflammatory protein-1 alpha with improved pharmaceutical properties."
Hunter M.G. , Bawden L. , Brotherton D. , Craig S. , Cribbes S. , Czaplewski L.G. , Dexter T.M. , Drummond A.H. , Gearing A.H. , Heyworth C.M. , Lord B.I. , Mccourt M. , Varley P.G. , Wood L.M. , Edwards R.M. , Lewis P.J.
Blood86:4400-4408(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 24-92;MUTAGENESIS OF ASP-49
8.

"Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells."
Cocchi F. , DeVico A.L. , Garzino-Demo A. , Arya S.K. , Gallo R.C. , Lusso P.
Science270:1811-1815(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 27-40 AND 71-83;FUNCTION
9.

"Identification of MIP-1 alpha/LD78 as a monocyte chemoattractant released by the HTLV-I-transformed cell line MT4."
Bertini R. , Luini W. , Sozzani S. , Bottazzi B. , Ruggiero P. , Boraschi D. , Saggioro D. , Chieco-Bianchi L. , Proost P. , van Damme J. , Mantovani A.
AIDS Res. Hum. Retroviruses11:155-160(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 27-51;IDENTIFICATION OF LD78-ALPHA(4-69)
10.

"Identification of a glycosaminoglycan-binding site in chemokine macrophage inflammatory protein-1alpha."
Koopmann W. , Krangel M.S.
J. Biol. Chem.272:10103-10109(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GAG BINDING SITES ARG-40; ARG-68 AND ARG-70;MUTAGENESIS OF ARG-40; ARG-68 AND ARG-70
11.

"Natural truncation of the chemokine MIP-1beta/CCL4 affects receptor specificity but not anti-HIV-1 activity."
Guan E. , Wang J. , Roderiquez G. , Norcross M.A.
J. Biol. Chem.277:32348-32352(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBUNIT;INTERACTION WITH MIP-1-BETA(3-69)
12.

"Macrophage inflammatory protein-1."
Menten P. , Wuyts A. , Van Damme J.
Cytokine Growth Factor Rev.13:455-481(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
13.

"Identification of amino acid residues critical for aggregation of human CC chemokines macrophage inflammatory protein (MIP)-1alpha, MIP-1beta, and RANTES. Characterization of active disaggregated chemokine variants."
Czaplewski L.G. , McKeating J. , Craven C.J. , Higgins L.D. , Appay V. , Brown A. , Dudgeon T. , Howard L.A. , Meyers T. , Owen J. , Palan S.R. , Tan P. , Wilson G. , Woods N.R. , Heyworth C.M. , Lord B.I. , Brotherton D. , Christison R. , more...
J. Biol. Chem.274:16077-16084(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 24-92;MUTAGENESIS OF ASP-49 AND GLU-89

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