CCL5 ELISA Kit for Human(Homo sapiens) | EIAab.comCCL5 ELISA Kit for Human(Homo sapiens)

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Index > Protein center > CCL5(Gene name) > Human

CCL5 (Gene name),

C-C motif chemokine 5 (Protein name ), CCL5_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

CCL5(D17S136E;SCYA5);

Protein name:

C-C motif chemokine 5;

Alternative:

Eosinophil chemotactic cytokine;EoCP;Small-inducible cytokine A5;SIS-delta;T-cell-specific protein RANTES;T cell-specific protein P228(TCP228);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

N/A

Function:

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

Sequence:

50:

91:

FYTSGKCSNP | AVVFVTRKNR | QVCANPEKKW | VREYINSLEM | S

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Hs.514821

Pfam:

PF00048

KEGG:

hsa:6352

STRING:

9606.ENSP00000293272

String:

P13501

SMR:

P13501

MIM:

187011

Uniprot:

P13501

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ELISA Kit for Human C-C motif chemokine 5

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E0116h

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96T

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ELISA Kit for Human C-C motif chemokine 5

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E0116m

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96T

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ELISA Kit for Human C-C motif chemokine 5

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E0116b

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ELISA Kit for Human C-C motif chemokine 5

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E0116p

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96T

ELISA Kit for Human C-C motif chemokine 5

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E0116r

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96T

ELISA Kit for Human C-C motif chemokine 5

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E0116d

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96T

CLIA Kit for Human C-C motif chemokine 5

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U0116h

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96T

CLIA Kit for Human C-C motif chemokine 5

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U0116p

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96T

CLIA Kit for Human C-C motif chemokine 5

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U0116d

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96T

CLIA Kit for Human C-C motif chemokine 5

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U0116r

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96T

CLIA Kit for Human C-C motif chemokine 5

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U0116b

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96T

CLIA Kit for Human C-C motif chemokine 5

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U0116m

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96T

Polyclonal Antibody for Human C-C motif chemokine 5

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P0116Rb-m

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40ug/0.2ml

Polyclonal Antibody for Human C-C motif chemokine 5

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P0116Rb-h

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40ug/0.2ml

Polyclonal Antibody for Human C-C motif chemokine 5

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P0116Rb-r

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40ug/0.2ml

Monoclonal Antibody for Human C-C motif chemokine 5

Protein for Human C-C motif chemokine 5

R&D Technical Data

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 8.1%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-103
Plasma	98	92-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-102%	90-103%	93-104%	92-106%
EDTA plasma(n=5)	89-97%	92-101%	93-99%	90-102%
heparin plasma(n=5)	91-102%	90-104%	95-107%	93-108%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"A human T cell-specific molecule is a member of a new gene family."
Schall T.J. , Jongstra J. , Dyer B.J. , Jorgensen J. , Clayberger C. , Davis M.M. , Krensky A.M.
J. Immunol.141:1018-1025(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"Organization of the chemokine gene cluster on human chromosome 17q11.2 containing the genes for CC chemokine MPIF-1, HCC-2, LEC, and RANTES."
Nomiyama H. , Fukuda S. , Iio M. , Tanase S. , Miura R. , Yoshie O.
J. Interferon Cytokine Res.19:227-234(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
3.

"A natural CCL5/RANTES variant antagonist for CCR1 and CCR3."
Capoulade-Metay C. , Ayouba A. , Kfutwah A. , Lole K. , Petres S. , Dudoit Y. , Deterre P. , Menu E. , Barre-Sinoussi F. , Debre P. , Theodorou I.
Immunogenetics58:533-541(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION;VARIANT PHE-24
tissue: Blood.
4.

Jang J.S. , Kim B.E.
Submitted (1998-01) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Leukocyte.
5.

"The complete sequence of human beta-chemokine RANTES mRNA."
Zeng Q.P. , Yang R.Y. , Fu L.C.
Submitted (2000-05) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
6.

NIEHS SNPs program
Submitted (2005-04) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
7.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Brain.
8.

"Cytokine RANTES released by thrombin-stimulated platelets is a potent attractant for human eosinophils."
Kameyoshi Y. , Doerschner A. , Mallet A.I. , Christophers E. , Schroeder J.-M.
J. Exp. Med.176:587-592(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 24-55;FUNCTION;MASS SPECTROMETRY;GLYCOSYLATION AT SER-27 AND SER-28;OXIDATION AT MET-90
9.

"Platelets secrete an eosinophil-chemotactic cytokine which is a member of the C-C-chemokine family."
Schroeder J.-M. , Kameyoshi Y. , Christophers E.
Adv. Exp. Med. Biol.351:119-128(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 24-55
10.

"Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells."
Cocchi F. , DeVico A.L. , Garzino-Demo A. , Arya S.K. , Gallo R.C. , Lusso P.
Science270:1811-1815(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 49-56; 71-79 AND 83-91;FUNCTION
11.

"Amino-terminal truncation of chemokines by CD26/dipeptidyl-peptidase IV. Conversion of RANTES into a potent inhibitor of monocyte chemotaxis and HIV-1-infection."
Proost P. , De Meester I. , Schols D. , Struyf S. , Lambeir A.-M. , Wuyts A. , Opdenakker G. , De Clercq E. , Scharpe S. , Van Damme J.
J. Biol. Chem.273:7222-7227(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION OF RANTES(3-68);PROTEOLYTIC PROCESSING OF N-TERMINUS;FUNCTION
12.

"Multiple pathways of amino terminal processing produce two truncated variants of RANTES/CCL5."
Lim J.K. , Burns J.M. , Lu W. , DeVico A.L.
J. Leukoc. Biol.78:442-452(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION OF RANTES(4-68);MASS SPECTROMETRY;FUNCTION
13.

"RANTES stimulates Ca2+ mobilization and inositol trisphosphate (IP3) formation in cells transfected with G protein-coupled receptor 75."
Ignatov A. , Robert J. , Gregory-Evans C. , Schaller H.C.
Br. J. Pharmacol.149:490-497(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
14.

"The novel chemokine receptor, G-protein-coupled receptor 75, is expressed by islets and is coupled to stimulation of insulin secretion and improved glucose homeostasis."
Liu B. , Hassan Z. , Amisten S. , King A.J. , Bowe J.E. , Huang G.C. , Jones P.M. , Persaud S.J.
Diabetologia56:2467-2476(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
15.

"Proton NMR assignments and solution conformation of RANTES, a chemokine of the C-C type."
Skelton N.J. , Aspiras F. , Ogez J. , Schall T.J.
Biochemistry34:5329-5342(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR
16.

"The three-dimensional solution structure of RANTES."
Chung C.-W. , Cooke R.M. , Proudfoot A.E.I. , Wells T.N.C.
Biochemistry34:9307-9314(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR
17.

"Total chemical synthesis and high-resolution crystal structure of the potent anti-HIV protein AOP-RANTES."
Wilken J. , Hoover D. , Thompson D.A. , Barlow P.N. , McSparron H. , Picard L. , Wlodawer A. , Lubkowski J. , Kent S.B.
Chem. Biol.6:43-51(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SYNTHESIS;X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS)
18.

"The crystal structure of Met-RANTES: comparison with native RANTES and AOP-RANTES."
Hoover D.M. , Shaw J. , Gryczynski Z. , Proudfoot A.E.I. , Wells T.N.C. , Lubkowski J.
Protein Pept. Lett.7:73-82(2000)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS)

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