CD8A ELISA Kit for Dog(Canis familiaris) | EIAab.comCD8A ELISA Kit for Dog(Canis familiaris)

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CD8A (Gene name),

T-cell surface glycoprotein CD8 alpha chain (Protein name ), CD8A_CANFA from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

CD8A;

Protein name:

T-cell surface glycoprotein CD8 alpha chain;

Alternative:

CD:

CD8a;

Organism:

Dog (Canis familiarisCanis lupus familiaris).

General Annotation

Sub Unit:

In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers.

Function:

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Subcellular Location:

Membrane Single-pass type I membrane protein

Protein Attributes:

Sequence length:

239

Sequence:

50:

100:

150:

200:

239:

LLSLVITIIC | NHRNRRRVCK | CPRPVVRPGG | KPSPSEKYV

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF07686

UniGene:

Cfa.3636

String:

P33706

KEGG:

cfa:403157

STRING:

9615.ENSCAFP00000011083

SMR:

P33706

Uniprot:

P33706

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ELISA Kit for Dog T-cell surface glycoprotein CD8 alpha chain

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E0953h

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ELISA Kit for Dog T-cell surface glycoprotein CD8 alpha chain

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E0953r

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ELISA Kit for Dog T-cell surface glycoprotein CD8 alpha chain

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E0953d

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ELISA Kit for Dog T-cell surface glycoprotein CD8 alpha chain

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E0953b

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96T

ELISA Kit for Dog T-cell surface glycoprotein CD8 alpha chain

Cat.:

E0953m

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96T

CLIA Kit for Dog T-cell surface glycoprotein CD8 alpha chain

Cat.:

U0953m

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96T

CLIA Kit for Dog T-cell surface glycoprotein CD8 alpha chain

Cat.:

U0953r

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Looking for manual.

MSDS:

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96T

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CLIA Kit for Dog T-cell surface glycoprotein CD8 alpha chain

Cat.:

U0953h

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Looking for manual.

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Packing:

96T

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CLIA Kit for Dog T-cell surface glycoprotein CD8 alpha chain

Cat.:

U0953b

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Packing:

96T

CLIA Kit for Dog T-cell surface glycoprotein CD8 alpha chain

Cat.:

U0953d

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Packing:

96T

Polyclonal Antibody for Dog T-cell surface glycoprotein CD8 alpha chain

Cat.:

P0953Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Dog T-cell surface glycoprotein CD8 alpha chain

Cat.:

P0953Rb-r

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40ug/0.2ml

Polyclonal Antibody for Dog T-cell surface glycoprotein CD8 alpha chain

Cat.:

P0953Rb-m

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Packing:

40ug/0.2ml

Monoclonal Antibody for Dog T-cell surface glycoprotein CD8 alpha chain

Protein for Dog T-cell surface glycoprotein CD8 alpha chain

R&D Technical Data

Western blot-Anti-CD8A antibody

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 4.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 9.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	93-103
Plasma	98	91-105

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-104%	92-99%	95-102%	89-103%
EDTA plasma(n=5)	88-96%	90-101%	87-103%	93-107%
heparin plasma(n=5)	94-102%	95-105%	98-109%	92-105%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Isolation and expression of cDNA encoding the canine CD4 and CD8 alpha antigens."
Gorman S.D. , Frewin M.R. , Cobbold S.P. , Waldmann H.
Tissue Antigens43:184-188(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Beagle.
tissue: Thymus.

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