CD8A ELISA Kit for Human(Homo sapiens) | EIAab.comCD8A ELISA Kit for Human(Homo sapiens)

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CD8A (Gene name),

T-cell surface glycoprotein CD8 alpha chain (Protein name ), CD8A_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

CD8A(MAL);

Protein name:

T-cell surface glycoprotein CD8 alpha chain;

Alternative:

T-lymphocyte differentiation antigen T8/Leu-2;

CD:

CD8a;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner.

Function:

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Subcellular Location:

Isoform 2 Secreted

Protein Attributes:

Sequence length:

235

Sequence:

50:

100:

150:

200:

235:

VITLYCNHRN | RRRVCKCPRP | VVKSGDKPSL | SARYV

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF07686

UniGene:

Hs.85258

MIM:

186910

KEGG:

hsa:925

String:

P01732

STRING:

9606.ENSP00000283635

SMR:

P01732

Uniprot:

P01732

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ELISA Kit for Human T-cell surface glycoprotein CD8 alpha chain

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E0953h

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96T

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ELISA Kit for Human T-cell surface glycoprotein CD8 alpha chain

Cat.:

E0953r

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96T

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ELISA Kit for Human T-cell surface glycoprotein CD8 alpha chain

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E0953d

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ELISA Kit for Human T-cell surface glycoprotein CD8 alpha chain

Cat.:

E0953b

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96T

ELISA Kit for Human T-cell surface glycoprotein CD8 alpha chain

Cat.:

E0953m

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96T

CLIA Kit for Human T-cell surface glycoprotein CD8 alpha chain

Cat.:

U0953m

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MSDS:

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Packing:

96T

CLIA Kit for Human T-cell surface glycoprotein CD8 alpha chain

Cat.:

U0953r

Price:

Please sign in first.

Manual:

Looking for manual.

MSDS:

Please sign in first.

Packing:

96T

Range:

Please sign in first.

CLIA Kit for Human T-cell surface glycoprotein CD8 alpha chain

Cat.:

U0953h

Price:

Please sign in first.

Manual:

Looking for manual.

MSDS:

Please sign in first.

Packing:

96T

Range:

Please sign in first.

CLIA Kit for Human T-cell surface glycoprotein CD8 alpha chain

Cat.:

U0953b

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MSDS:

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Packing:

96T

CLIA Kit for Human T-cell surface glycoprotein CD8 alpha chain

Cat.:

U0953d

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human T-cell surface glycoprotein CD8 alpha chain

Cat.:

P0953Rb-h

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40ug/0.2ml

Polyclonal Antibody for Human T-cell surface glycoprotein CD8 alpha chain

Cat.:

P0953Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human T-cell surface glycoprotein CD8 alpha chain

Cat.:

P0953Rb-m

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human T-cell surface glycoprotein CD8 alpha chain

Protein for Human T-cell surface glycoprotein CD8 alpha chain

R&D Technical Data

Western blot-Anti-CD8A antibody

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 4.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 9.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	93-103
Plasma	98	91-105

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-104%	92-99%	95-102%	89-103%
EDTA plasma(n=5)	88-96%	90-101%	87-103%	93-107%
heparin plasma(n=5)	94-102%	95-105%	98-109%	92-105%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"The isolation and sequence of the gene encoding T8: a molecule defining functional classes of T lymphocytes."
Littman D.R. , Thomas Y. , Maddon P.J. , Chess L. , Axel R.
Cell40:237-246(1985) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
2.

"Structure of Leu-2/T8 as deduced from the sequence of a cDNA clone."
Parnes J.R. , Sizer K.C. , Sukhatme V.P. , Hunkapiller T.
Behring Inst. Mitt.77:48-55(1985) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1)
3.

"The T cell differentiation antigen Leu-2/T8 is homologous to immunoglobulin and T cell receptor variable regions."
Sukhatme V.P. , Sizer K.C. , Vollmer A.C. , Hunkapiller T. , Parnes J.R.
Cell40:591-597(1985) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
4.

"Structure and expression of the gene encoding CD8 alpha chain (Leu-2/T8)."
Nakayama K. , Tokito S. , Okumura K. , Nakauchi H.
Immunogenetics30:393-397(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
5.

"Alternatively spliced mRNA encodes a secreted form of human CD8 alpha. Characterization of the human CD8 alpha gene."
Norment A.M. , Lonberg N. , Lacy E. , Littman D.R.
J. Immunol.142:3312-3319(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2);ALTERNATIVE SPLICING
6.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3)
tissue: Pericardium.
7.

"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W. , Graves T.A. , Fulton R.S. , Fulton L.A. , Pepin K.H. , Minx P. , Wagner-McPherson C. , Layman D. , Wylie K. , Sekhon M. , Becker M.C. , Fewell G.A. , Delehaunty K.D. , Miner T.L. , Nash W.E. , Kremitzki C. , Oddy L. , Du H. , more...
Nature434:724-731(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
8.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
9.

"A secreted form of the human lymphocyte cell surface molecule CD8 arises from alternative splicing."
Giblin P. , Ledbetter J.A. , Kavathas P.
Proc. Natl. Acad. Sci. U.S.A.86:998-1002(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE OF 168-235 (ISOFORMS 1 AND 2)
10.

"The T8 antigen is a multimeric complex of two distinct subunits on human thymocytes but consists of homomultimeric forms on peripheral blood T lymphocytes."
Snow P.M. , Terhorst C.
J. Biol. Chem.258:14675-14681(1983) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBUNIT
11.

"Crystal structure of a soluble form of the human T cell coreceptor CD8 at 2.6-A resolution."
Leahy D.J. , Axel R. , Hendrickson W.A.
Cell68:1145-1162(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 22-135
12.

"Crystal structure of the complex between human CD8alpha(alpha) and HLA-A2."
Gao G.F. , Tormo J. , Gerth U.C. , Wyer J.R. , McMichael A.J. , Stuart D.I. , Bell J.I. , Jones E.Y. , Jakobsen B.K.
Nature387:630-634(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) OF 22-141 IN COMPLEX WITH HLA-A/B2M DIMER
13.

"A zinc clasp structure tethers Lck to T cell coreceptors CD4 and CD8."
Kim P.W. , Sun Z.Y. , Blacklow S.C. , Wagner G. , Eck M.J.
Science301:1725-1728(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 209-227 IN COMPLEX WITH LCK
14.

"Familial CD8 deficiency due to a mutation in the CD8 alpha gene."
de la Calle-Martin O. , Hernandez M. , Ordi J. , Casamitjana N. , Arostegui J.I. , Caragol I. , Ferrando M. , Labrador M. , Rodriguez-Sanchez J.L. , Espanol T.
J. Clin. Invest.108:117-123(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT CD8 DEFICIENCY SER-111;MUTAGENESIS OF GLY-111

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