Protein name:
    E3 ubiquitin-protein ligase CHFR ;
    
    Alternative:
    
    RING finger protein 196 ;Checkpoint with forkhead and RING finger domains protein ;
    
    
 
    Organism:
        
        
        
        
        
        
    
        Human  (Homo sapiens).  
    
 
General Annotation
    Sub Unit:
    Interacts with HDAC1 and HDAC2. Interacts with PML (with sumoylated form of PML).
    
 
    Function:
    E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.
    
 
    
        
        Subcellular Location:
        
    
     
     
      Nucleus  
      PML body 
     
  
    
 
    
    Protein Attributes:
    
    50:
    MERPEEGKQS |  PPPQPWGRLL |  RLGAEEGEPH |  VLLRKREWTI |  GRRRGCDLSF |  
    
 
    100:
    PSNKLVSGDH |  CRIVVDEKSG |  QVTLEDTSTS |  GTVINKLKVV |  KKQTCPLQTG |  
    
 
    150:
    DVIYLVYRKN |  EPEHNVAYLY |  ESLSEKQGMT |  QESFEANKEN |  VFHGTKDTSG |  
    
 
    200:
    AGAGRGADPR |  VPPSSPATQV |  CFEEPQPSTS |  TSDLFPTASA |  SSTEPSPAGR |  
    
 
    250:
    ERSSSCGSGG |  GGISPKGSGP |  SVASDEVSSF |  ASALPDRKTA |  SFSSLEPQDQ |  
    
 
    300:
    EDLEPVKKKM |  RGDGDLDLNG |  QLLVAQPRRN |  AQTVHEDVRA |  AAGKPDKMEE |  
    
 
    350:
    TLTCIICQDL |  LHDCVSLQPC |  MHTFCAACYS |  GWMERSSLCP |  TCRCPVERIC |  
    
 
    400:
    KNHILNNLVE |  AYLIQHPDKS |  RSEEDVQSMD |  ARNKITQDML |  QPKVRRSFSD |  
    
 
    450:
    EEGSSEDLLE |  LSDVDSESSD |  ISQPYVVCRQ |  CPEYRRQAAQ |  PPHCPAPEGE |  
    
 
    500:
    PGAPQALGDA |  PSTSVSLTTA |  VQDYVCPLQG |  SHALCTCCFQ |  PMPDRRAERE |  
    
 
    550:
    QDPRVAPQQC |  AVCLQPFCHL |  YWGCTRTGCY |  GCLAPFCELN |  LGDKCLDGVL |  
    
 
    600:
    NNNSYESDIL |  KNYLATRGLT |  WKNMLTESLV |  ALQRGVFLLS |  DYRVTGDTVL |  
    
 
    650:
    CYCCGLRSFR |  ELTYQYRQNI |  PASELPVAVT |  SRPDCYWGRN |  CRTQVKAHHA |  
    
 
      Vaild Sequence:
    
 
Related Databases
Uniprot:
 
 
    
        
        ELISA Kit
         
        
        CLIA Kit
         
        
        Polyclonal Antibody
         
        
        Monoclonal Antibody
         
        
        Protein
         
        
     
FOR
    
        
        Mouse
         
        
        Human
         
        
     
    
        ELISA Kit for Human E3 ubiquitin-protein ligase CHFR
        
     
    
    
    
    
    
    
 
    
        ELISA Kit for Human E3 ubiquitin-protein ligase CHFR
        
     
    
    
    
    
    
    
 
    
        CLIA Kit for Human E3 ubiquitin-protein ligase CHFR
        
     
    
    
    
    
    
    
 
    
        CLIA Kit for Human E3 ubiquitin-protein ligase CHFR
        
     
    
    
    
    
    
    
 
    
        Polyclonal Antibody for Human E3 ubiquitin-protein ligase CHFR
        
     
    
    
    
    
    
    
 
    
        Polyclonal Antibody for Human E3 ubiquitin-protein ligase CHFR
        
     
    
    
    
    
    
    
 
    
        Monoclonal Antibody for Human E3 ubiquitin-protein ligase CHFR
        
     
    
    
    
    
    
    
 
    
        Monoclonal Antibody for Human E3 ubiquitin-protein ligase CHFR
        
     
    
    
    
    
    
    
 
    
        Protein for Human E3 ubiquitin-protein ligase CHFR
        
     
    
    
    
    
    
    
 
    
        Protein for Human E3 ubiquitin-protein ligase CHFR
        
     
    
    
    
    
    
    
 
 
 
 
 
 
 
 
    R&D Technical Data
    
    
    
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    Precision
    
    
    
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    Recovery
    
    
    
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    Linearity
    
    
    
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    References
    
    
        
            
        
            
                1.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);FUNCTION;TISSUE SPECIFICITY;VARIANT MET-580 
                    
                    
                 
             
         
            
        
            
                2.
                
                    
                        
                            "Complete sequencing and characterization of 21,243 full-length human cDNAs." 
                        
                        
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Sekine M. 
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Obayashi M. 
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Nishi T. 
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                              more...   
                             
                        
                        
                        
                            Nat. Genet.36:40-45(2004)
                         
                        
                        
                        [
PubMed ]
                        [
Europe PMC ]
                        [
Abstract ]
                        
                    
 
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4);VARIANT MET-580 
                    
                    
                    tissue :
                    
Teratocarcinoma . tissue :
                    
Testis . tissue :
                    
Trachea .  
             
         
            
        
            
                3.
                
                    
                        
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                            Williamson A.L. 
                        ,
                            Wleczyk R. 
                        ,
                            Wooden H.S. 
                        ,
                            Wooden S.H. 
                        ,
                            Yen J. 
                        ,
                            Yoon L. 
                        ,
                            Yoon V. 
                        ,
                            Zorrilla S.E. 
                        ,
                            Nelson D. 
                        ,
                            Kucherlapati R. 
                        ,
                            Weinstock G. 
                        ,
                            Gibbs R.A. 
                        
                             
                              more...   
                             
                        
                        
                        
                            Nature440:346-351(2006)
                         
                        
                        
                        [
PubMed ]
                        [
Europe PMC ]
                        [
Abstract ]
                        
                    
 
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] 
                    
                    
                 
             
         
            
        
            
                4.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2);VARIANT VAL-497 
                    
                    
                    tissue :
                    
Placenta .  
             
         
            
        
            
                5.
                
                    
                        
                            "The full-ORF clone resource of the German cDNA consortium." 
                        
                        
                            Bechtel S. 
                        ,
                            
Rosenfelder H. 
                        ,
                            
Duda A. 
                        ,
                            
Schmidt C.P. 
                        ,
                            
Ernst U. 
                        ,
                            
Wellenreuther R. 
                        ,
                            
Mehrle A. 
                        ,
                            
Schuster C. 
                        ,
                            
Bahr A. 
                        ,
                            
Bloecker H. 
                        ,
                            
Heubner D. 
                        ,
                            
Hoerlein A. 
                        ,
                            
Michel G. 
                        ,
                            
Wedler H. 
                        ,
                            
Koehrer K. 
                        ,
                            
Ottenwaelder B. 
                        ,
                            
Poustka A. 
                        ,
                            
Wiemann S. 
                        ,
                        
                        
                            Schupp I. 
                        
                             
                              more...   
                             
                        
                        
                        
                            BMC Genomics8:399-399(2007)
                         
                        
                        
                        [
PubMed ]
                        [
Europe PMC ]
                        [
Abstract ]
                        
                    
 
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 359-664 
                    
                    
                    tissue :
                    
Testis .  
             
         
            
        
            
                6.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for FUNCTION;AUTOUBIQUITINATION;MUTAGENESIS OF ILE-306 AND TRP-332 
                    
                    
                 
             
         
            
        
            
                7.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for FUNCTION;AUTOUBIQUITINATION;SUBCELLULAR LOCATION;DEVELOPMENTAL STAGE 
                    
                    
                 
             
         
            
        
            
                8.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for VARIANTS ARG-270; VAL-497 AND MET-580;SILENCING IN PRIMARY CANCERS 
                    
                    
                 
             
         
            
        
            
                9.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for SILENCING IN PRIMARY CANCERS 
                    
                    
                 
             
         
            
        
            
                10.
                
                    
                        
                            "Epigenetic inactivation of CHFR in human tumors." 
                        
                        
                            Toyota M. 
                        ,
                            
Sasaki Y. 
                        ,
                            
Satoh A. 
                        ,
                            
Ogi K. 
                        ,
                            
Kikuchi T. 
                        ,
                            
Suzuki H. 
                        ,
                            
Mita H. 
                        ,
                            
Tanaka N. 
                        ,
                            
Itoh F. 
                        ,
                            
Issa J.-P.J. 
                        ,
                            
Jair K.-W. 
                        ,
                            
Schuebel K.E. 
                        ,
                            
Imai K. 
                        ,
                            
Tokino T. 
                        
                        
                        
                        
                            Proc. Natl. Acad. Sci. U.S.A.100:7818-7823(2003)
                         
                        
                        
                        [
PubMed ]
                        [
Europe PMC ]
                        [
Abstract ]
                        
                    
 
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for SILENCING IN PRIMARY CANCERS 
                    
                    
                 
             
         
            
        
            
                11.
                
                    
                        
                            "Epigenetic inactivation of CHFR and sensitivity to microtubule inhibitors in gastric cancer." 
                        
                        
                            Satoh A. 
                        ,
                            
Toyota M. 
                        ,
                            
Itoh F. 
                        ,
                            
Sasaki Y. 
                        ,
                            
Suzuki H. 
                        ,
                            
Ogi K. 
                        ,
                            
Kikuchi T. 
                        ,
                            
Mita H. 
                        ,
                            
Yamashita T. 
                        ,
                            
Kojima T. 
                        ,
                            
Kusano M. 
                        ,
                            
Fujita M. 
                        ,
                            
Hosokawa M. 
                        ,
                            
Endo T. 
                        ,
                            
Tokino T. 
                        ,
                            
Imai K. 
                        
                        
                        
                        
                            Cancer Res.63:8606-8613(2003)
                         
                        
                        
                        [
PubMed ]
                        [
Europe PMC ]
                        [
Abstract ]
                        
                    
 
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for SILENCING IN PRIMARY CANCERS 
                    
                    
                 
             
         
            
        
            
                12.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for PHOSPHORYLATION;MUTAGENESIS OF THR-39 AND SER-205 
                    
                    
                 
             
         
            
        
            
                13.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for FUNCTION;INTERACTION WITH UBE2V2;PHOSPHORYLATION 
                    
                    
                 
             
         
            
        
            
                14.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for FUNCTION 
                    
                    
                 
             
         
            
        
            
                15.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for SUBCELLULAR LOCATION;INTERACTION WITH PML 
                    
                    
                 
             
         
            
        
            
                16.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for FUNCTION;SUBCELLULAR LOCATION;DOMAIN PBZ-TYPE;POLY-ADP-RIBOSYLATION;ADP-RIBOSE-BINDING;MUTAGENESIS OF ARG-632; CYS-635; CYS-641; ARG-642 AND GLN-644 
                    
                    
                 
             
         
            
        
            
                17.
                
                    
                    
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                        Cited for DOMAIN FHA 
                    
                    
                 
             
         
            
        
            
                18.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for FUNCTION;INTERACTION WITH HDAC1 AND HDAC2;AUTOUBIQUITINATION;MUTAGENESIS OF ILE-306 
                    
                    
                 
             
         
            
        
            
                19.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 14-128 
                    
                    
                 
             
         
            
        
            
                20.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for VARIANTS LEU-166; PRO-202 AND SER-536