Language:
  
[Sign in] [Register]   

EIAab logo

Index > Protein center > CHFR(Gene name) > Human
  • Add your favorite
  • CHFR (Gene name),
  • E3 ubiquitin-protein ligase CHFR (Protein name ),  CHFR_HUMAN from NCBI database.
  • top
  • Come back to page head.
  • |
  • General Annotation
  • |
  • Antigen Annotation
  • |
  • 3D
  • |
  • Predicted Eptitope
  • |
  • Vaild Sequence
  • |
  • Related Databases
  • |
  • Feedback
  • Gene name:
    CHFR(RNF196);
    Protein name:
    E3 ubiquitin-protein ligase CHFR;
    Alternative:
    RING finger protein 196;Checkpoint with forkhead and RING finger domains protein;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    Interacts with HDAC1 and HDAC2. Interacts with PML (with sumoylated form of PML).
    Function:
    E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.
    Subcellular Location:
    Nucleus PML body
    Protein Attributes:
    Sequence length:
    664
    Sequence:
    50:
    MERPEEGKQS | PPPQPWGRLL | RLGAEEGEPH | VLLRKREWTI | GRRRGCDLSF | 
    100:
    PSNKLVSGDH | CRIVVDEKSG | QVTLEDTSTS | GTVINKLKVV | KKQTCPLQTG | 
    150:
    DVIYLVYRKN | EPEHNVAYLY | ESLSEKQGMT | QESFEANKEN | VFHGTKDTSG | 
    200:
    AGAGRGADPR | VPPSSPATQV | CFEEPQPSTS | TSDLFPTASA | SSTEPSPAGR | 
    250:
    ERSSSCGSGG | GGISPKGSGP | SVASDEVSSF | ASALPDRKTA | SFSSLEPQDQ | 
    300:
    EDLEPVKKKM | RGDGDLDLNG | QLLVAQPRRN | AQTVHEDVRA | AAGKPDKMEE | 
    350:
    TLTCIICQDL | LHDCVSLQPC | MHTFCAACYS | GWMERSSLCP | TCRCPVERIC | 
    400:
    KNHILNNLVE | AYLIQHPDKS | RSEEDVQSMD | ARNKITQDML | QPKVRRSFSD | 
    450:
    EEGSSEDLLE | LSDVDSESSD | ISQPYVVCRQ | CPEYRRQAAQ | PPHCPAPEGE | 
    500:
    PGAPQALGDA | PSTSVSLTTA | VQDYVCPLQG | SHALCTCCFQ | PMPDRRAERE | 
    550:
    QDPRVAPQQC | AVCLQPFCHL | YWGCTRTGCY | GCLAPFCELN | LGDKCLDGVL | 
    600:
    NNNSYESDIL | KNYLATRGLT | WKNMLTESLV | ALQRGVFLLS | DYRVTGDTVL | 
    650:
    CYCCGLRSFR | ELTYQYRQNI | PASELPVAVT | SRPDCYWGRN | CRTQVKAHHA | 
    664:
    MKFNHICEQT | RFKN
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    String:
    SMR:
    MIM:
    Pfam:
    UniGene:
    KEGG:
    Uniprot:
     
    FOR
    ELISA Kit for Human E3 ubiquitin-protein ligase CHFR
    ELISA Kit for Human E3 ubiquitin-protein ligase CHFR
    CLIA Kit for Human E3 ubiquitin-protein ligase CHFR
    CLIA Kit for Human E3 ubiquitin-protein ligase CHFR
    Polyclonal Antibody for Human E3 ubiquitin-protein ligase CHFR
    Polyclonal Antibody for Human E3 ubiquitin-protein ligase CHFR
    Monoclonal Antibody for Human E3 ubiquitin-protein ligase CHFR
    Monoclonal Antibody for Human E3 ubiquitin-protein ligase CHFR
    Protein for Human E3 ubiquitin-protein ligase CHFR
    Protein for Human E3 ubiquitin-protein ligase CHFR

    R&D Technical Data
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Precision
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      "Chfr defines a mitotic stress checkpoint that delays entry into metaphase."
      Scolnick D.M. , Halazonetis T.D.
      Nature406:430-435(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);FUNCTION;TISSUE SPECIFICITY;VARIANT MET-580
    2. 2.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4);VARIANT MET-580
      tissue: Teratocarcinoma.
      tissue: Testis.
      tissue: Trachea.
    3. 3.
      "The finished DNA sequence of human chromosome 12."
      Scherer S.E. , Muzny D.M. , Buhay C.J. , Chen R. , Cree A. , Ding Y. , Dugan-Rocha S. , Gill R. , Gunaratne P. , Harris R.A. , Hawes A.C. , Hernandez J. , Hodgson A.V. , Hume J. , Jackson A. , Khan Z.M. , Kovar-Smith C. , Lewis L.R. , more...
      Nature440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    4. 4.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2);VARIANT VAL-497
      tissue: Placenta.
    5. 5.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 359-664
      tissue: Testis.
    6. 6.
      "The checkpoint protein Chfr is a ligase that ubiquitinates Plk1 and inhibits Cdc2 at the G2 to M transition."
      Kang D. , Chen J. , Wong J. , Fang G.
      J. Cell Biol.156:249-259(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;AUTOUBIQUITINATION;MUTAGENESIS OF ILE-306 AND TRP-332
    7. 7.
      "Chfr regulates a mitotic stress pathway through its RING-finger domain with ubiquitin ligase activity."
      Chaturvedi P. , Sudakin V. , Bobiak M.L. , Fisher P.W. , Mattern M.R. , Jablonski S.A. , Hurle M.R. , Zhu Y. , Yen T.J. , Zhou B.-B.
      Cancer Res.62:1797-1801(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;AUTOUBIQUITINATION;SUBCELLULAR LOCATION;DEVELOPMENTAL STAGE
    8. 8.
      "Aberrant hypermethylation of the CHFR prophase checkpoint gene in human lung cancers."
      Mizuno K. , Osada H. , Konishi H. , Tatematsu Y. , Yatabe Y. , Mitsudomi T. , Fujii Y. , Takahashi T.
      Oncogene21:2328-2333(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS ARG-270; VAL-497 AND MET-580;SILENCING IN PRIMARY CANCERS
    9. 9.
      "Frequent hypermethylation of the 5' CpG island of the mitotic stress checkpoint gene Chfr in colorectal and non-small cell lung cancer."
      Corn P.G. , Summers M.K. , Fogt F. , Virmani A.K. , Gazdar A.F. , Halazonetis T.D. , El-Deiry W.S.
      Carcinogenesis24:47-51(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SILENCING IN PRIMARY CANCERS
    10. 10.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SILENCING IN PRIMARY CANCERS
    11. 11.
      "Epigenetic inactivation of CHFR and sensitivity to microtubule inhibitors in gastric cancer."
      Satoh A. , Toyota M. , Itoh F. , Sasaki Y. , Suzuki H. , Ogi K. , Kikuchi T. , Mita H. , Yamashita T. , Kojima T. , Kusano M. , Fujita M. , Hosokawa M. , Endo T. , Tokino T. , Imai K.
      Cancer Res.63:8606-8613(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SILENCING IN PRIMARY CANCERS
    12. 12.
      "Promotion of mitosis by activated protein kinase B after DNA damage involves polo-like kinase 1 and checkpoint protein CHFR."
      Shtivelman E.
      Mol. Cancer Res.1:959-969(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION;MUTAGENESIS OF THR-39 AND SER-205
    13. 13.
      "The Chfr mitotic checkpoint protein functions with Ubc13-Mms2 to form Lys63-linked polyubiquitin chains."
      Bothos J. , Summers M.K. , Venere M. , Scolnick D.M. , Halazonetis T.D.
      Oncogene22:7101-7107(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH UBE2V2;PHOSPHORYLATION
    14. 14.
      "CHFR-associated early G2/M checkpoint defects in breast cancer cells."
      Erson A.E. , Petty E.M.
      Mol. Carcinog.39:26-33(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
    15. 15.
      "PML bodies control the nuclear dynamics and function of the CHFR mitotic checkpoint protein."
      Daniels M.J. , Marson A. , Venkitaraman A.R.
      Nat. Struct. Mol. Biol.11:1114-1121(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;INTERACTION WITH PML
    16. 16.
      "Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins."
      Ahel I. , Ahel D. , Matsusaka T. , Clark A.J. , Pines J. , Boulton S.J. , West S.C.
      Nature451:81-85(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;DOMAIN PBZ-TYPE;POLY-ADP-RIBOSYLATION;ADP-RIBOSE-BINDING;MUTAGENESIS OF ARG-632; CYS-635; CYS-641; ARG-642 AND GLN-644
    17. 17.
      "The anti-proliferative effects of the CHFR depend on the forkhead associated domain, but not E3 ligase activity mediated by ring finger domain."
      Fukuda T. , Kondo Y. , Nakagama H.
      PLoS ONE3:E1776-E1776(2008) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DOMAIN FHA
    18. 18.
      "Chfr is linked to tumour metastasis through the downregulation of HDAC1."
      Oh Y.M. , Kwon Y.E. , Kim J.M. , Bae S.J. , Lee B.K. , Yoo S.J. , Chung C.H. , Deshaies R.J. , Seol J.H.
      Nat. Cell Biol.11:295-302(2009) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH HDAC1 AND HDAC2;AUTOUBIQUITINATION;MUTAGENESIS OF ILE-306
    19. 19.
      "Crystal structure of the FHA domain of the Chfr mitotic checkpoint protein and its complex with tungstate."
      Stavridi E.S. , Huyen Y. , Loreto I.R. , Scolnick D.M. , Halazonetis T.D. , Pavletich N.P. , Jeffrey P.D.
      Structure10:891-899(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 14-128
    20. 20.
      "Inactivating mutations targeting the chfr mitotic checkpoint gene in human lung cancer."
      Mariatos G. , Bothos J. , Zacharatos P. , Summers M.K. , Scolnick D.M. , Kittas C. , Halazonetis T.D. , Gorgoulis V.G.
      Cancer Res.63:7185-7189(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS LEU-166; PRO-202 AND SER-536
    Product Feedback Wall
    Hot Genes
    Atf2 ASPRO ACE ALCAM C19orf80 Trap1a Gdf5
    Top Searches
    Ubiquitin-protein ligase metalloproteinase Ubiquitin ELISA Alpha Tumor necrosis Asprosin TRAP1A
    Why choose EIAAB
    Our products have been quoted by many publications in famous journals such as Cell; Cell Metabolism; Hepatology; Biomaterials.more
    Further Information
    About us Protein center Bank account Distributors Terms & Conditions Career eiaab.com.cn

    Copyright & copy www.eiaab.com2006-2016 All Rights Reserved    EIAab         Email:eiaab@eiaab.com

    鄂ICP备10015095号-1

    鄂公网安备 42018502005535号

    Twitter