Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.
Polyclonal Antibody for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
Polyclonal Antibody for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
Monoclonal Antibody for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
Monoclonal Antibody for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
Monoclonal Antibody for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
Protein for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
Protein for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
Protein for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
R&D Technical Data
s
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.9%
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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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Recovery
Recovery was determined by spiking various levels of CP21B into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
92
89-103
Plasma
93
86-102
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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CP21B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
87-97%
85-101%
83-103%
86-97%
EDTA plasma(n=5)
89-98%
87-99%
89-96%
90-95%
heparin plasma(n=5)
85-100%
86-101%
84-102%
88-108%
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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
5.
"Targeted disruption of the 25-hydroxyvitamin D3 1 a-hydroxylase gene in a Ras-transformed human keratinocyte cell line: evidence for an autocrine growth regulatory function of 1 alpha, 25-dihydroxyvitamin D3 in vitro and in vivo." Huang D.C.
,
Papavasiliou J.
,
Rhim J.
,
Kremer R.
Submitted (2000-03) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [MRNA]
6.
NIEHS SNPs program
Submitted (2003-04) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT LEU-166
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Cited for: VARIANTS VDDR1A HIS-107; GLU-125; PRO-335 AND SER-382
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Cited for: VARIANTS VDDR1A HIS-65; LYS-189; HIS-389; ILE-409; PRO-429; CYS-453 AND ARG-497
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Cited for: VARIANTS VDDR1A GLY-189; PHE-343; GLY-389; HIS-389 AND ILE-409