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Index > Protein center > CYP27B1(Gene name) > Human
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  • CYP27B1 (Gene name),
  • 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (Protein name ),  CP27B_HUMAN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Gene name:
    CYP27B1(CYP1ALPHA;CYP27B);
    Protein name:
    25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial;
    Alternative:
    25-hydroxyvitamin D(3) 1-alpha-hydroxylase(VD3 1A hydroxylase);25-OHD-1 alpha-hydroxylase;Cytochrome P450 subfamily XXVIIB polypeptide 1;Calcidiol 1-monooxygenase;Cytochrome P450VD1-alpha;Cytochrome P450C1 alpha;Cytochrome p450 27B1;
    Organism:
    Human (Homo sapiens). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.
    Subcellular Location:
    Mitochondrion membrane
    Protein Attributes:
    Sequence length:
    508
    Sequence:
    50:
    MTQTLKYASR | VFHRVRWAPE | LGASLGYREY | HSARRSLADI | PGPSTPSFLA | 
    100:
    ELFCKGGLSR | LHELQVQGAA | HFGPVWLASF | GTVRTVYVAA | PALVEELLRQ | 
    150:
    EGPRPERCSF | SPWTEHRRCR | QRACGLLTAE | GEEWQRLRSL | LAPLLLRPQA | 
    200:
    AARYAGTLNN | VVCDLVRRLR | RQRGRGTGPP | ALVRDVAGEF | YKFGLEGIAA | 
    250:
    VLLGSRLGCL | EAQVPPDTET | FIRAVGSVFV | STLLTMAMPH | WLRHLVPGPW | 
    300:
    GRLCRDWDQM | FAFAQRHVER | REAEAAMRNG | GQPEKDLESG | AHLTHFLFRE | 
    350:
    ELPAQSILGN | VTELLLAGVD | TVSNTLSWAL | YELSRHPEVQ | TALHSEITAA | 
    400:
    LSPGSSAYPS | ATVLSQLPLL | KAVVKEVLRL | YPVVPGNSRV | PDKDIHVGDY | 
    450:
    IIPKNTLVTL | CHYATSRDPA | QFPEPNSFRP | ARWLGEGPTP | HPFASLPFGF | 
    500:
    GKRSCMGRRL | AELELQMALA | QILTHFEVQP | EPGAAPVRPK | TRTVLVPERS | 
    508:
    INLQFLDR
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    MIM:
    KEGG:
    Pfam:
    UniGene:
    String:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
    Cat.:
    E10324h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
    Cat.:
    E10324r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
    Cat.:
    E10324m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
    Cat.:
    U10324r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
    Cat.:
    U10324m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
    Cat.:
    U10324h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
    Cat.:
    P10324Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
    Polyclonal Antibody for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
    Monoclonal Antibody for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
    Monoclonal Antibody for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
    Monoclonal Antibody for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
    Protein for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
    Protein for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
    Protein for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

    R&D Technical Data

    s

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    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
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    Precision

    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤6.3%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.9%

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Recovery

    Recovery was determined by spiking various levels of CP21B into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    92

    89-103

    Plasma

    93

    86-102

     

     

     

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    Linearity

     

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CP21B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    87-97%

    85-101%

    83-103%

    86-97%

    EDTA plasma(n=5)

    89-98%

    87-99%

    89-96%

    90-95%

    heparin plasma(n=5)

    85-100%

     

    86-101%

    84-102%

    88-108%

     

    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.
    References
    1. 1.
      "Complete structure of the human gene for the vitamin D 1alpha-hydroxylase, P450c1alpha."
      Fu G.K. , Portale A.P. , Miller W.L.
      DNA Cell Biol.16:1499-1507(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    2. 2.
      "Molecular cloning of cDNA and genomic DNA for human 25-hydroxyvitamin D3 1 alpha-hydroxylase."
      Monkawa T. , Yoshida T. , Wakino S. , Shinki T. , Anazawa H. , Deluca H.F. , Suda T. , Hayashi M. , Saruta T.
      Biochem. Biophys. Res. Commun.239:527-533(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]
      tissue: Kidney.
    3. 3.
      "Cloning of human 25-hydroxyvitamin D-1 alpha-hydroxylase and mutations causing vitamin D-dependent rickets type 1."
      Fu G.K. , Lin D. , Zhang Y.H. , Bikle D.D. , Shackleton C.H. , Miller W.L. , Portale A.A.
      Mol. Endocrinol.11:1961-1970(1997) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    4. 4.
      "Targeted disruption of the 25-hydroxyvitamin D3 1alpha-hydroxylase gene in ras-transformed keratinocytes demonstrates that locally produced 1alpha,25-dihydroxyvitamin D3 suppresses growth and induces differentiation in an autocrine fashion."
      Huang D.C. , Papavasiliou V. , Rhim J.S. , Horst R.L. , Kremer R.
      Mol. Cancer Res.1:56-67(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
    5. 5.
      "Targeted disruption of the 25-hydroxyvitamin D3 1 a-hydroxylase gene in a Ras-transformed human keratinocyte cell line: evidence for an autocrine growth regulatory function of 1 alpha, 25-dihydroxyvitamin D3 in vitro and in vivo."
      Huang D.C. , Papavasiliou J. , Rhim J. , Kremer R.
      Submitted (2000-03) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    6. 6.
      NIEHS SNPs program
      Submitted (2003-04) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT LEU-166
    7. 7.
      "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
      Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
      tissue: Kidney.
    8. 8.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
    9. 9.
      "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
    10. 10.
      "Inactivating mutations in the 25-hydroxyvitamin D3 1alpha-hydroxylase gene in patients with pseudovitamin D-deficiency rickets."
      Kitanaka S. , Takeyama K. , Murayama A. , Sato T. , Okumura K. , Nogami M. , Hasegawa Y. , Niimi H. , Yanagisawa J. , Tanaka T. , Kato S.
      N. Engl. J. Med.338:653-661(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS VDDR1A HIS-107; GLU-125; PRO-335 AND SER-382
    11. 11.
      "Genetics of vitamin D 1-alpha-hydroxylase deficiency in 17 families."
      Wang J.T. , Lin C.-J. , Burridge S.M. , Fu G.K. , Labuda M. , Portale A.A. , Miller W.L.
      Am. J. Hum. Genet.63:1694-1702(1998) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS VDDR1A HIS-65; LYS-189; HIS-389; ILE-409; PRO-429; CYS-453 AND ARG-497
    12. 12.
      "Novel mutations in the 1alpha-hydroxylase (P450c1) gene in three families with pseudovitamin D-deficiency rickets resulting in loss of functional enzyme activity in blood-derived macrophages."
      Smith S.J. , Rucka A.K. , Berry J.L. , Davies M. , Mylchreest S. , Paterson C.R. , Heath D.A. , Tassabehji M. , Read A.P. , Mee A.P. , Mawer E.B.
      J. Bone Miner. Res.14:730-739(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS VDDR1A TYR-323 AND GLY-478
    13. 13.
      "No enzyme activity of 25-hydroxyvitamin D3 1alpha-hydroxylase gene product in pseudovitamin D deficiency rickets, including that with mild clinical manifestation."
      Kitanaka S. , Murayama A. , Sakaki T. , Inouye K. , Seino Y. , Fukumoto S. , Shima M. , Yukizane S. , Takayanagi M. , Niimi H. , Takeyama K. , Kato S.
      J. Clin. Endocrinol. Metab.84:4111-4117(1999) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS VDDR1A ARG-321 AND CYS-389
    14. 14.
      "Novel gene mutations in patients with 1alpha-hydroxylase deficiency that confer partial enzyme activity in vitro."
      Wang X. , Zhang M.Y. , Miller W.L. , Portale A.A.
      J. Clin. Endocrinol. Metab.87:2424-2430(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS VDDR1A GLY-189; PHE-343; GLY-389; HIS-389 AND ILE-409
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