CYP3A4 ELISA Kit for Human(Homo sapiens) | EIAab.comCYP3A4 ELISA Kit for Human(Homo sapiens)

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CYP3A4 (Gene name),

Cytochrome P450 3A4 (Protein name ), CP3A4_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

CYP3A4(CYP3A3);

Protein name:

Cytochrome P450 3A4;

Alternative:

Quinine 3-monooxygenase;1.14.13.97;1.14.13.32;Taurochenodeoxycholate 6-alpha-hydroxylase(Albendazole sulfoxidase);1.14.13.67;1.14.13.157;Taurochenodeoxycholate 6-alpha-hydroxylase(Albendazole monooxygenase);CYPIIIA4;CYPIIIA3;Cytochrome P450 HLp;Cytochrome P450 3A3;Cytochrome P450-PCN1;Cytochrome P450 NF-25;Nifedipine oxidase;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

N/A

Function:

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.

Subcellular Location:

Endoplasmic reticulum membrane Single-pass membrane protein Microsome membrane Single-pass membrane protein

Protein Attributes:

Sequence length:

503

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

503:

SGA

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

MIM:

124010

STRING:

9606.ENSP00000337915

UniGene:

Hs.728751

SMR:

P08684

Pfam:

PF00067

String:

P08684

KEGG:

hsa:1576

Uniprot:

P08684

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ELISA Kit for Human Cytochrome P450 3A4

Cat.:

E0217h

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Manual:

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MSDS:

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Packing:

96T

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CLIA Kit for Human Cytochrome P450 3A4

Cat.:

U0217h

Price:

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Manual:

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MSDS:

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Packing:

96T

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Polyclonal Antibody for Human Cytochrome P450 3A4

Cat.:

P0217Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human Cytochrome P450 3A4

Protein for Human Cytochrome P450 3A4

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

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References

1.

"Complete cDNA sequence of a cytochrome P-450 inducible by glucocorticoids in human liver."
Molowa D.T. , Schuetz E.G. , Wrighton S.A. , Watkins P.B. , Kremers P. , Mendez-Picon G. , Parker G.A. , Guzelian P.S.
Proc. Natl. Acad. Sci. U.S.A.83:5311-5315(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT ALA-252
tissue: Liver.
2.

"Human P450PCN1: sequence, chromosome localization, and direct evidence through cDNA expression that P450PCN1 is nifedipine oxidase."
Gonzalez F.J. , Schmid B.J. , Umeno M. , McBride O.W. , Hardwick J.P. , Meyer U.A. , Gelboin H.V. , Idle J.R.
DNA7:79-86(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"Isolation and sequence determination of a cDNA clone related to human cytochrome P-450 nifedipine oxidase."
Beaune P.H. , Umbenhauer D.R. , Bork R.W. , Lloyd R.S. , Guengerich F.P.
Proc. Natl. Acad. Sci. U.S.A.83:8064-8068(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT THR-431
4.

"The human cytochrome P450 CYP3 locus: assignment to chromosome 7q22-qter."
Spurr N.K. , Gough A.C. , Stevenson K. , Wolf C.R.
Hum. Genet.81:171-174(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
5.

"Characterization of mRNA species related to human liver cytochrome P-450 nifedipine oxidase and the regulation of catalytic activity."
Bork R.W. , Muto T. , Beaune P.H. , Srivastava P.K. , Lloyd R.S. , Guengerich F.P.
J. Biol. Chem.264:910-919(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANT THR-431
tissue: Liver.
6.

"Establishment of transgenic cell line CHL-3A4 and its metabolic activation."
Chen Q. , Wu J. , Yu Y.
Zhonghua Yu Fang Yi Xue Za Zhi32:281-284(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Liver.
7.

"Genomic organization of the human CYP3A locus: identification of a new, inducible CYP3A gene."
Gellner K. , Eiselt R. , Hustert E. , Arnold H. , Koch I. , Haberl M. , Deglmann C.J. , Burk O. , Buntefuss D. , Escher S. , Bishop C. , Koebe H.-G. , Brinkmann U. , Klenk H.-P. , Kleine K. , Meyer U.A. , Wojnowski L.
Pharmacogenetics11:111-121(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
8.

"Novel mutations of CYP3A4 in Chinese."
Hsieh K.-P. , Lin Y.-Y. , Cheng C.-L. , Lai M.-L. , Lin M.-S. , Siest J.-P. , Huang J.-D.
Drug Metab. Dispos.29:268-273(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS VAL-118 AND ARG-218
9.

"Sequence of a new human cytochrome P450-3A4 cDNA."
Zhuge J. , Qian Y. , Xie H. , Yu Y.
Submitted (1999-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Liver.
10.

"Cytochrome P-450 in human liver microsomes: high-performance liquid chromatographic isolation of three forms and their characterization."
Komori M. , Hashizume T. , Ohi H. , Miura T. , Kitada M. , Nagashima K. , Kamataki T.
J. Biochem.104:912-916(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-25;CLEAVAGE OF INITIATOR METHIONINE
11.

"Identification of an inducible form of cytochrome P-450 in human liver."
Watkins P.B. , Wrighton S.A. , Maurel P. , Schuetz E.G. , Mendez-Picon G. , Parker G.A. , Guzelian P.S.
Proc. Natl. Acad. Sci. U.S.A.82:6310-6314(1985) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-21;CLEAVAGE OF INITIATOR METHIONINE
tissue: Liver.
12.

"Cytochrome P-450 hPCN3, a novel cytochrome P-450 IIIA gene product that is differentially expressed in adult human liver. cDNA and deduced amino acid sequence and distinct specificities of cDNA-expressed hPCN1 and hPCN3 for the metabolism of steroid hormones and cyclosporine."
Aoyama T. , Yamano S. , Waxman D.J. , Lapenson D.P. , Meyer U.A. , Fischer V. , Tyndale R. , Inaba T. , Kalow W. , Gelboin H.V. , Gonzalez F.J.
J. Biol. Chem.264:10388-10395(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY
13.

"Interindividual variations in human liver cytochrome P-450 enzymes involved in the oxidation of drugs, carcinogens and toxic chemicals: studies with liver microsomes of 30 Japanese and 30 Caucasians."
Shimada T. , Yamazaki H. , Mimura M. , Inui Y. , Guengerich F.P.
J. Pharmacol. Exp. Ther.270:414-423(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY
14.

"CYP3A gene expression in human gut epithelium."
Kolars J.C. , Lown K.S. , Schmiedlin-Ren P. , Ghosh M. , Fang C. , Wrighton S.A. , Merion R.M. , Watkins P.B.
Pharmacogenetics4:247-259(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY
15.

"Evidence for involvement of human CYP3A in the 3-hydroxylation of quinine."
Zhang H. , Coville P.F. , Walker R.J. , Miners J.O. , Birkett D.J. , Wanwimolruk S.
Br. J. Clin. Pharmacol.43:245-252(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION
16.

"Mutual inhibition between quinine and etoposide by human liver microsomes. Evidence for cytochrome P4503A4 involvement in their major metabolic pathways."
Zhao X.J. , Kawashiro T. , Ishizaki T.
Drug Metab. Dispos.26:188-191(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION
17.

"Oxidation of 1,8-cineole, the monoterpene cyclic ether originated from eucalyptus polybractea, by cytochrome P450 3A enzymes in rat and human liver microsomes."
Miyazawa M. , Shindo M. , Shimada T.
Drug Metab. Dispos.29:200-205(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CATALYTIC ACTIVITY;FUNCTION AS 1,8-CINEOLE 2-EXO-MONOOXYGENASE
18.

"Intergenic mRNA molecules resulting from trans-splicing."
Finta C. , Zaphiropoulos P.G.
J. Biol. Chem.277:5882-5890(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TRANS-SPLICING
19.

"ABC transporters, cytochromes P450 and their main transcription factors: expression at the human blood-brain barrier."
Dauchy S. , Dutheil F. , Weaver R.J. , Chassoux F. , Daumas-Duport C. , Couraud P.O. , Scherrmann J.M. , De Waziers I. , Decleves X.
J. Neurochem.107:1518-1528(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY
20.

"Drug metabolism in human brain: high levels of cytochrome P4503A43 in brain and metabolism of anti-anxiety drug alprazolam to its active metabolite."
Agarwal V. , Kommaddi R.P. , Valli K. , Ryder D. , Hyde T.M. , Kleinman J.E. , Strobel H.W. , Ravindranath V.
PLoS ONE3:E2337-E2337(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY
21.

"CYP3A4 ubiquitination by gp78 (the tumor autocrine motility factor receptor, AMFR) and CHIP E3 ligases."
Pabarcus M.K. , Hoe N. , Sadeghi S. , Patterson C. , Wiertz E. , Correia M.A.
Arch. Biochem. Biophys.483:66-74(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION
22.

"Xenobiotic-metabolizing enzymes and transporters in the normal human brain: regional and cellular mapping as a basis for putative roles in cerebral function."
Dutheil F. , Dauchy S. , Diry M. , Sazdovitch V. , Cloarec O. , Mellottee L. , Bieche I. , Ingelman-Sundberg M. , Flinois J.P. , de Waziers I. , Beaune P. , Decleves X. , Duyckaerts C. , Loriot M.A.
Drug Metab. Dispos.37:1528-1538(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TISSUE SPECIFICITY
23.

"The structure of human microsomal cytochrome P450 3A4 determined by X-ray crystallography to 2.05-A resolution."
Yano J.K. , Wester M.R. , Schoch G.A. , Griffin K.J. , Stout C.D. , Johnson E.F.
J. Biol. Chem.279:38091-38094(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 24-503
24.

"Crystal structures of human cytochrome P450 3A4 bound to metyrapone and progesterone."
Williams P.A. , Cosme J. , Vinkovic D.M. , Ward A. , Angove H.C. , Day P.J. , Vonrhein C. , Tickle I.J. , Jhoti H.
Science305:683-686(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) OF 25-503
25.

"CYP3A4 allelic variants with amino acid substitutions in exons 7 and 12: evidence for an allelic variant with altered catalytic activity."
Sata F. , Sapone A. , Elizondo G. , Stocker P. , Miller V.P. , Zheng W. , Raunio H. , Crespi C.L. , Gonzalez F.J.
Clin. Pharmacol. Ther.67:48-56(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS PRO-222 AND THR-445
26.

"Identification of variants of CYP3A4 and characterization of their abilities to metabolize testosterone and chlorpyrifos."
Dai D. , Tang J. , Rose R. , Hodgson E. , Bienstock R.J. , Mohrenweiser H.W. , Goldstein J.A.
J. Pharmacol. Exp. Ther.299:825-831(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS GLN-162; SER-189; PRO-293; THR-445 AND SER-467;CHARACTERIZATION OF THE VARIANTS
27.

"Identification and functional characterization of eight CYP3A4 protein variants."
Eiselt R. , Domanski T.L. , Zibat A. , Mueller R. , Presecan-Siedel E. , Hustert E. , Zanger U.M. , Brockmoller J. , Klenk H.-P. , Meyer U.A. , Khan K.K. , He Y.-A. , Halpert J.R. , Wojnowski L.
Pharmacogenetics11:447-458(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS ASP-56; GLN-130; ILE-170; HIS-174; MET-363; PHE-373 AND LEU-416
28.

"Common allelic variants of cytochrome P4503A4 and their prevalence in different populations."
Lamba J.K. , Lin Y.S. , Thummel K. , Daly A. , Watkins P.B. , Strom S. , Zhang J. , Schuetz E.G.
Pharmacogenetics12:121-132(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS PRO-15; GLN-162; HIS-174; SER-185 AND PHE-373
29.

"Genetic variation in eleven phase I drug metabolism genes in an ethnically diverse population."
Solus J.F. , Arietta B.J. , Harris J.R. , Sexton D.P. , Steward J.Q. , McMunn C. , Ihrie P. , Mehall J.M. , Edwards T.L. , Dawson E.P.
Pharmacogenomics5:895-931(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS HIS-174; SER-185 AND PRO-293

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