CYP3A5 ELISA Kit for Human(Homo sapiens) | EIAab.comCYP3A5 ELISA Kit for Human(Homo sapiens)

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CYP3A5 (Gene name),

Cytochrome P450 3A5 (Protein name ), CP3A5_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

CYP3A5;

Protein name:

Cytochrome P450 3A5;

Alternative:

Cytochrome P450 HLp2;CYPIIIA5;Cytochrome P450-PCN3;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

N/A

Function:

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Subcellular Location:

Endoplasmic reticulum membrane Peripheral membrane protein Microsome membrane Peripheral membrane protein

Protein Attributes:

Sequence length:

502

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

502:

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

P20815

UniGene:

Hs.571258

Pfam:

PF00067

SMR:

P20815

MIM:

605325

KEGG:

hsa:1577

STRING:

9606.ENSP00000222982

Uniprot:

P20815

FOR

ELISA Kit for Human Cytochrome P450 3A5

Cat.:

E12438h

Price:

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Manual:

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MSDS:

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Packing:

96T

Range:

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CLIA Kit for Human Cytochrome P450 3A5

Cat.:

U12438h

Price:

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human Cytochrome P450 3A5

Cat.:

P12438Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human Cytochrome P450 3A5

Protein for Human Cytochrome P450 3A5

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Cytochrome P-450 hPCN3, a novel cytochrome P-450 IIIA gene product that is differentially expressed in adult human liver. cDNA and deduced amino acid sequence and distinct specificities of cDNA-expressed hPCN1 and hPCN3 for the metabolism of steroid hormones and cyclosporine."
Aoyama T. , Yamano S. , Waxman D.J. , Lapenson D.P. , Meyer U.A. , Fischer V. , Tyndale R. , Inaba T. , Kalow W. , Gelboin H.V. , Gonzalez F.J.
J. Biol. Chem.264:10388-10395(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
2.

"Characterization of a cDNA encoding a new member of the glucocorticoid-responsive cytochromes P450 in human liver."
Schuetz J.D. , Molowa D.T. , Guzelian P.S.
Arch. Biochem. Biophys.274:355-365(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);CHARACTERIZATION
tissue: Liver.
3.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
4.

"Human chromosome 7: DNA sequence and biology."
Scherer S.W. , Cheung J. , MacDonald J.R. , Osborne L.R. , Nakabayashi K. , Herbrick J.-A. , Carson A.R. , Parker-Katiraee L. , Skaug J. , Khaja R. , Zhang J. , Hudek A.K. , Li M. , Haddad M. , Duggan G.E. , Fernandez B.A. , Kanematsu E. , Gentles S. , more...
Science300:767-772(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

"The DNA sequence of human chromosome 7."
Hillier L.W. , Fulton R.S. , Fulton L.A. , Graves T.A. , Pepin K.H. , Wagner-McPherson C. , Layman D. , Maas J. , Jaeger S. , Walker R. , Wylie K. , Sekhon M. , Becker M.C. , O'Laughlin M.D. , Schaller M.E. , Fewell G.A. , Delehaunty K.D. , Miner T.L. , more...
Nature424:157-164(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
7.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue: Colon.
8.

"Genomic organization of the human CYP3A locus: identification of a new, inducible CYP3A gene."
Gellner K. , Eiselt R. , Hustert E. , Arnold H. , Koch I. , Haberl M. , Deglmann C.J. , Burk O. , Buntefuss D. , Escher S. , Bishop C. , Koebe H.-G. , Brinkmann U. , Klenk H.-P. , Kleine K. , Meyer U.A. , Wojnowski L.
Pharmacogenetics11:111-121(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-106
9.

"Identification of a novel dexamethasone responsive enhancer in the human CYP3A5 gene and its activation in human and rat liver cells."
Schuetz J.D. , Schuetz E.G. , Thottassery J.V. , Guzelian P.S. , Strom S. , Sun D.
Mol. Pharmacol.49:63-72(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24
10.

"Sequence of the 5'-flanking region of CYP3A5: comparative analysis with CYP3A4 and CYP3A7."
Jounaidi Y. , Guzelian P.S. , Maurel P. , Vilarem M.J.
Biochem. Biophys. Res. Commun.205:1741-1747(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24
11.

"Intergenic mRNA molecules resulting from trans-splicing."
Finta C. , Zaphiropoulos P.G.
J. Biol. Chem.277:5882-5890(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TRANS-SPLICING
12.

"Detection of CYP3A5 allelic variant: a candidate for the polymorphic expression of the protein?"
Jounaidi Y. , Hyrailles V. , Gervot L. , Maurel P.
Biochem. Biophys. Res. Commun.221:466-470(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT CYP3A5*2 ASN-398
13.

"Genetic polymorphism of cytochrome P450 3A5 in Chinese."
Chou F.C. , Tzeng S.J. , Huang J.D.
Drug Metab. Dispos.29:1205-1209(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT CYP3A5*4 ARG-200
14.

"Genetic findings and functional studies of human CYP3A5 single nucleotide polymorphisms in different ethnic groups."
Lee S.J. , Usmani K.A. , Chanas B. , Ghanayem B. , Xi T. , Hodgson E. , Mohrenweiser H.W. , Goldstein J.A.
Pharmacogenetics13:461-472(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS CYS-28; THR-337 AND SER-446
15.

"Genetic variation in eleven phase I drug metabolism genes in an ethnically diverse population."
Solus J.F. , Arietta B.J. , Harris J.R. , Sexton D.P. , Steward J.Q. , McMunn C. , Ihrie P. , Mehall J.M. , Edwards T.L. , Dawson E.P.
Pharmacogenomics5:895-931(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS TYR-30; GLU-277; THR-337 AND ASN-398

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