Eef1a1 ELISA Kit for Rat(Rattus norvegicus) | EIAab.comEef1a1 ELISA Kit for Rat(Rattus norvegicus) | EIAab.com

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Eef1a1 (Gene name),

Elongation factor 1-alpha 1 (Protein name ), EF1A1_RAT from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Gene name:

Eef1a1(Eef1a);

Protein name:

Elongation factor 1-alpha 1(EF-1-alpha-1);

Alternative:

Eukaryotic elongation factor 1 A-1(eEF1A-1);Elongation factor Tu(EF-Tu);

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA. Interacts with PARP1, TXK, XPO5 and KARS (By similarity). May interact with ERGIC2.

Function:

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

Subcellular Location:

Cytoplasm Nucleus

Protein Attributes:

Sequence length:

462

Sequence:

50:

MGKEKTHINI | VVIGHVDSGK | STTTGHLIYK | CGGIDKRTIE | KFEKEAAEMG |

100:

KGSFKYAWVL | DKLKAERERG | ITIDISLWKF | ETSKYYVTII | DAPGHRDFIK |

150:

NMITGTSQAD | CAVLIVAAGV | GEFEAGISKN | GQTREHALLA | YTLGVKQLIV |

200:

GVNKMDSTEP | PYSQKRYEEI | VKEVSTYIKK | IGYNPDTVAF | VPISGWNGDN |

250:

MLEPSANMPW | FKGWKVTRKD | GSASGTTLLE | ALDCILPPTR | PTDKPLRLPL |

300:

QDVYKIGGIG | TVPVGRVETG | VLKPGMVVTF | APVNVTTEVK | SVEMHHEALS |

350:

EALPGDNVGF | NVKNVSVKDV | RRGNVAGDSK | NDPPMEAAGF | TAQVIILNHP |

400:

GQISAGYAPV | LDCHTAHIAC | KFAELKEKID | RRSGKKLEDG | PKFLKSGDAA |

450:

IVDMVPGKPM | CVESFSDYPP | LGRFAVRDMR | QTVAVGVIKA | VDKKAAGAGK |

462:

VTKSAQKAQK | AK

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

String:

UniGene:

SMR:

KEGG:

Uniprot:

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ELISA Kit for Rat EF-1-alpha-1

MSDS:

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ELISA Kit for Rat EF-1-alpha-1

Cat.:

E11476m

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Packing:

96T

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ELISA Kit for Rat EF-1-alpha-1

Cat.:

E11476r

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MSDS:

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Packing:

96T

ELISA Kit for Rat EF-1-alpha-1

Cat.:

E11476h

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96T

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ELISA Kit for Rat EF-1-alpha-1

MSDS:

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CLIA Kit for Rat EF-1-alpha-1

Cat.:

U11476h

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MSDS:

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Packing:

96T

Range:

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CLIA Kit for Rat EF-1-alpha-1

Cat.:

U11476m

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Rat EF-1-alpha-1

Cat.:

U11476r

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Rat EF-1-alpha-1

MSDS:

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CLIA Kit for Rat EF-1-alpha-1

MSDS:

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Polyclonal Antibody for Rat EF-1-alpha-1

Polyclonal Antibody for Rat EF-1-alpha-1

Polyclonal Antibody for Rat EF-1-alpha-1

Polyclonal Antibody for Rat EF-1-alpha-1

Polyclonal Antibody for Rat EF-1-alpha-1

Cat.:

P11476Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Rat EF-1-alpha-1

Monoclonal Antibody for Rat EF-1-alpha-1

Monoclonal Antibody for Rat EF-1-alpha-1

Monoclonal Antibody for Rat EF-1-alpha-1

Monoclonal Antibody for Rat EF-1-alpha-1

Protein for Rat EF-1-alpha-1

Protein for Rat EF-1-alpha-1

Protein for Rat EF-1-alpha-1

Protein for Rat EF-1-alpha-1

Protein for Rat EF-1-alpha-1

R&D Technical Data

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s

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s

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Precision

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

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Recovery

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Recovery was determined by spiking various levels of EF1A1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	101	97-109
Plasma	103	95-106

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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Linearity

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EF1A1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	94-108%	97-105%	92-110%	95-106%
EDTA plasma(n=5)	95-110%	96-109%	95-111%	97-108%
heparin plasma(n=5)	97-107%	99-107%	98-108%	94-112%

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

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References

1.

"Nucleotide sequence of rat elongation factor-1 alpha cDNA."
Shirasawa T. , Sakamoto K. , Akashi T. , Takahashi H. , Kawashima A.
Nucleic Acids Res.20:909-909(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Wistar.
2.

"Rat elongation factor 1 alpha: sequence of cDNA from a highly metastatic fos-transferred cell line."
Taniguchi S. , Miyamoto S. , Hiroyuki S. , Kobayashi H.
Nucleic Acids Res.19:6949-6949(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"Cloning of human and mouse brain cDNAs coding for S1, the second member of the mammalian elongation factor-1 alpha gene family: analysis of a possible evolutionary pathway."
Lee S. , Ann D.K. , Wang E.
Biochem. Biophys. Res. Commun.203:1371-1377(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Sprague-Dawley.
4.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Brain.
tissue: Heart.
tissue: Ovary.
tissue: Pituitary.
tissue: Spleen.
5.

"Characterization of the statin-like S1 and rat elongation factor 1 alpha as two distinctly expressed messages in rat."
Ann D.K. , Lin H.H. , Lee S. , Tu Z.-J. , Wang E.
J. Biol. Chem.267:699-702(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 256-462
tissue: Parotid gland.

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