EPO ELISA Kit for Human(Homo sapiens) | EIAab.comEPO ELISA Kit for Human(Homo sapiens)

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Index > Protein center > EPO(Gene name) > Human

EPO (Gene name),

Erythropoietin (Protein name ), EPO_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

EPO;

Protein name:

Erythropoietin;

Alternative:

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

N/A

Function:

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

193

Sequence:

50:

100:

150:

193:

AASAAPLRTI | TADTFRKLFR | VYSNFLRGKL | KLYTGEACRT | GDR

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

MIM:

133170

Pfam:

PF00758

UniGene:

Hs.2303

KEGG:

hsa:2056

String:

P01588

STRING:

9606.ENSP00000252723

SMR:

P01588

Uniprot:

P01588

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ELISA Kit for Human Erythropoietin

Cat.:

E0028r

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96T

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ELISA Kit for Human Erythropoietin

Cat.:

E0028h

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96T

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ELISA Kit for Human Erythropoietin

Cat.:

E0028d

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ELISA Kit for Human Erythropoietin

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E0028p

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ELISA Kit for Human Erythropoietin

Cat.:

E0028m

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ELISA Kit for Human Erythropoietin

Cat.:

E0028b

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96T

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ELISA Kit for Human Erythropoietin

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CLIA Kit for Human Erythropoietin

Cat.:

U0028b

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Packing:

96T

CLIA Kit for Human Erythropoietin

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CLIA Kit for Human Erythropoietin

Cat.:

U0028m

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96T

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CLIA Kit for Human Erythropoietin

Cat.:

U0028h

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Looking for manual.

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96T

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CLIA Kit for Human Erythropoietin

Cat.:

U0028d

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96T

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CLIA Kit for Human Erythropoietin

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U0028p

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Looking for manual.

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96T

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CLIA Kit for Human Erythropoietin

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U0028r

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Polyclonal Antibody for Human Erythropoietin

Cat.:

P0028Rb-h

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40ug/0.2ml

Polyclonal Antibody for Human Erythropoietin

Cat.:

P0028Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human Erythropoietin

Cat.:

P0028Rb-m

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40ug/0.2ml

Polyclonal Antibody for Human Erythropoietin

Monoclonal Antibody for Human Erythropoietin

Protein for Human Erythropoietin

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.1%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of EPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	94-99
Plasma	98	97-102

Recovery was determined by spiking various levels of EPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of EPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	95-103
Plasma	98	97-105

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EPO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-100%	90-98%	95-102%	92-97%
EDTA plasma(n=5)	89-95%	93-98%	99-105%	90-97%
heparin plasma(n=5)	93-98%	99-105%	101-107%	96-105%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-99%	94-98%	95-100%	100-105%
EDTA plasma(n=5)	91-95%	93-99%	89-94%	96-100%
heparin plasma(n=5)	93-96%	99-106%	97-100%	94-102%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-102%	95-101%	98-104%	97-105%
EDTA plasma(n=5)	96-99%	97-104%	100-105%	89-94%
heparin plasma(n=5)	91-96%	95-100%	99-107%	101-109%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Isolation and characterization of genomic and cDNA clones of human erythropoietin."
Jacobs K. , Shoemaker C. , Rudersdorf R. , Neill S.D. , Kaufman R.J. , Mufson A. , Seehra J. , Jones S.S. , Hewick R. , Fritsch E.F. , Kawakita M. , Shimizu T. , Miyake T.
Nature313:806-810(1985) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]
2.

"Cloning and expression of the human erythropoietin gene."
Lin F.-K. , Suggs S. , Lin C.-H. , Browne J.K. , Smalling R. , Egrie J.C. , Chen K.K. , Fox G.M. , Martin F. , Stabinsky Z. , Badrawi S.M. , Lai P.-H. , Goldwasser E.
Proc. Natl. Acad. Sci. U.S.A.82:7580-7584(1985) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
3.

"Large-scale sequencing of two regions in human chromosome 7q22: analysis of 650 kb of genomic sequence around the EPO and CUTL1 loci reveals 17 genes."
Gloeckner G. , Scherer S. , Schattevoy R. , Boright A.P. , Weber J. , Tsui L.-C. , Rosenthal A.
Genome Res.8:1060-1073(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
4.

"Erythropoietin gene sequence in the Quechua, a high altitude native population."
Rupert J.L. , Hochachka P.W.
Submitted (1999-11) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
5.

"The DNA sequence of human chromosome 7."
Hillier L.W. , Fulton R.S. , Fulton L.A. , Graves T.A. , Pepin K.H. , Wagner-McPherson C. , Layman D. , Maas J. , Jaeger S. , Walker R. , Wylie K. , Sekhon M. , Becker M.C. , O'Laughlin M.D. , Schaller M.E. , Fewell G.A. , Delehaunty K.D. , Miner T.L. , more...
Nature424:157-164(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
6.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Brain.
7.

"Gene expression of mutant erythropoietin in hepatocellular carcinoma."
Funakoshi A. , Muta H. , Baba T. , Shimizu S.
Biochem. Biophys. Res. Commun.195:717-722(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 58-193;VARIANTS HEPATOCELLULAR CARCINOMA 131-ASN-PHE-132 AND GLN-149
8.

"Structural characterization of human erythropoietin."
Lai P.H. , Everett R. , Wang F.F. , Arakawa T. , Goldwasser E.
J. Biol. Chem.261:3116-3121(1986) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 28-193;DISULFIDE BONDS
tissue: Urine.
9.

"Isolation of human erythropoietin with monoclonal antibodies."
Yanagawa S. , Hirade K. , Ohnota H. , Sasaki R. , Chiba H. , Ueda M. , Goto M.
J. Biol. Chem.259:2707-2710(1984) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PRELIMINARY PROTEIN SEQUENCE OF 28-57
10.

"Comparative study of the asparagine-linked sugar chains of human erythropoietins purified from urine and the culture medium of recombinant Chinese hamster ovary cells."
Takeuchi M. , Takasaki S. , Miyazaki H. , Kato T. , Hoshi S. , Kochibe N. , Kobata A.
J. Biol. Chem.263:3657-3663(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE OF CARBOHYDRATES
11.

"Site-specific glycosylation of human recombinant erythropoietin: analysis of glycopeptides or peptides at each glycosylation site by fast atom bombardment mass spectrometry."
Sasaki H. , Ochi N. , Dell A. , Fukuda M.
Biochemistry27:8618-8626(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE OF CARBOHYDRATES
12.

"Structures and functional roles of the sugar chains of human erythropoietins."
Takeuchi M. , Kobata A.
Glycobiology1:337-346(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE OF CARBOHYDRATES
13.

"Sugar profiling proves that human serum erythropoietin differs from recombinant human erythropoietin."
Skibeli V. , Nissen-Lie G. , Torjesen P.
Blood98:3626-3634(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE OF CARBOHYDRATES
14.

"Efficiency of signalling through cytokine receptors depends critically on receptor orientation."
Syed R.S. , Reid S.W. , Li C. , Cheetham J.C. , Aoki K.H. , Liu B. , Zhan H. , Osslund T.D. , Chirino A.J. , Zhang J. , Finer-Moore J. , Elliott S. , Sitney K. , Katz B.A. , Matthews D.J. , Wendoloski J.J. , Egrie J. , Stroud R.M.
Nature395:511-516(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 28-193
15.

"NMR structure of human erythropoietin and a comparison with its receptor bound conformation."
Cheetham J.C. , Smith D.M. , Aoki K.H. , Stevenson J.L. , Hoeffel T.J. , Syed R.S. , Egrie J. , Harvey T.S.
Nat. Struct. Biol.5:861-866(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 28-193
16.

"Promoter polymorphism of the erythropoietin gene in severe diabetic eye and kidney complications."
Genetics of diabetes and diabetic complication study group , Tong Z. , Yang Z. , Patel S. , Chen H. , Gibbs D. , Yang X. , Hau V.S. , Kaminoh Y. , Harmon J. , Pearson E. , Buehler J. , Chen Y. , Yu B. , Tinkham N.H. , Zabriskie N.A. , Zeng J. , Luo L. , more...
Proc. Natl. Acad. Sci. U.S.A.105:6998-7003(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN SUSCEPTIBILITY TO MICROVASCULAR COMPLICATIONS OF DIABETES TYPE 2

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