Protein name:
    Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) ;
    
    Alternative:
    
    2.6.99.-;Peptidyl-cysteine S-nitrosylase GAPDH ) ;
    
    
 
    Organism:
        
        
        
        
        
        
    
        Mouse  (Mus musculus).  
    
 
General Annotation
    Sub Unit:
    Homotetramer. Interacts with EIF1AD, USP25, PRKCI and WARS. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation.
    
 
    Function:
    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
    
 
    
        
        Subcellular Location:
        
    
     
     
      Cytoplasm  
      cytosol 
      
     
      Nucleus 
      
    Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. 
  
    
 
    
    Protein Attributes:
    
    50:
    MVKVGVNGFG |  RIGRLVTRAA |  ICSGKVEIVA |  INDPFIDLNY |  MVYMFQYDST |  
    
 
    100:
    HGKFNGTVKA |  ENGKLVINGK |  PITIFQERDP |  TNIKWGEAGA |  EYVVESTGVF |  
    
 
    150:
    TTMEKAGAHL |  KGGAKRVIIS |  APSADAPMFV |  MGVNHEKYDN |  SLKIVSNASC |  
    
 
    200:
    TTNCLAPLAK |  VIHDNFGIVE |  GLMTTVHAIT |  ATQKTVDGPS |  GKLWRDGRGA |  
    
 
    250:
    AQNIIPASTG |  AAKAVGKVIP |  ELNGKLTGMA |  FRVPTPNVSV |  VDLTCRLEKP |  
    
 
    300:
    AKYDDIKKVV |  KQASEGPLKG |  ILGYTEDQVV |  SCDFNSNSHS |  STFDAGAGIA |  
    
 
    333:
    LNDNFVKLIS |  WYDNEYGYSN |  RVVDLMAYMA |  SKE
    
 
      Vaild Sequence:
    
 
Related Databases
Uniprot:
 
 
    
        
        ELISA Kit
         
        
        CLIA Kit
         
        
        Polyclonal Antibody
         
        
        Monoclonal Antibody
         
        
        Protein
         
        
     
FOR
    
        
        Mouse
         
        
        Human
         
        
        Dog
         
        
        Rat
         
        
        Rabbit
         
        
        Pig
         
        
        Bovine
         
        
        Chicken
         
        
     
    
        ELISA Kit for Mouse GAPDH
        
     
    
    
    
    
   
    
    
    
    
    
    
    
    
    
    
    
    
 
    
        ELISA Kit for Mouse GAPDH
        
     
    
    
    
    
   
    
    
    
    
    
    
    
    
    
    
    
    
 
    
        ELISA Kit for Mouse GAPDH
        
     
    
    
    
    
   
    
    
    
    
    
    
    
    
    
    
    
    
 
    
        ELISA Kit for Mouse GAPDH
        
     
    
    
    
    
   
    
    
    
    
    
    
    
    
    
    
    
    
 
    
        ELISA Kit for Mouse GAPDH
        
     
    
    
    
    
    
    
    
    
 
    
        ELISA Kit for Mouse GAPDH
        
     
    
    
    
    
   
    
    
    
    
    
    
    
    
 
    
        ELISA Kit for Mouse GAPDH
        
     
    
    
    
    
   
    
    
    
    
    
    
    
    
 
    
        ELISA Kit for Mouse GAPDH
        
     
    
    
    
    
   
    
    
    
    
    
    
    
    
 
    
        Polyclonal Antibody for Mouse GAPDH
        
     
    
    
    
    
   
    
    
    
    
    
    
 
    
        Polyclonal Antibody for Mouse GAPDH
        
     
    
    
    
    
   
    
    
    
    
    
    
 
    
        Polyclonal Antibody for Mouse GAPDH
        
     
    
    
    
    
    
    
 
    
        Polyclonal Antibody for Mouse GAPDH
        
     
    
    
    
    
    
    
 
    
        Polyclonal Antibody for Mouse GAPDH
        
     
    
    
    
    
    
    
 
    
        Polyclonal Antibody for Mouse GAPDH
        
     
    
    
    
    
    
    
 
    
        Polyclonal Antibody for Mouse GAPDH
        
     
    
    
    
    
    
    
 
    
        Polyclonal Antibody for Mouse GAPDH
        
     
    
    
    
    
   
    
    
    
    
    
    
 
    
        Monoclonal Antibody for Mouse GAPDH
        
     
    
    
    
    
    
    
 
    
        Monoclonal Antibody for Mouse GAPDH
        
     
    
    
    
    
    
    
 
    
        Monoclonal Antibody for Mouse GAPDH
        
     
    
    
    
    
    
    
 
    
        Monoclonal Antibody for Mouse GAPDH
        
     
    
    
    
    
    
    
 
    
        Monoclonal Antibody for Mouse GAPDH
        
     
    
    
    
    
    
    
 
    
        Monoclonal Antibody for Mouse GAPDH
        
     
    
    
    
    
    
    
 
    
        Monoclonal Antibody for Mouse GAPDH
        
     
    
    
    
    
    
    
 
    
        Monoclonal Antibody for Mouse GAPDH
        
     
    
    
    
    
    
    
 
 
 
 
 
 
 
 
    R&D Technical Data
    
    
    
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    Precision
    
    
    
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    References
    
    
        
            
        
            
                1.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for NUCLEOTIDE SEQUENCE [MRNA] 
                    
                    
                 
             
         
            
        
            
                2.
                
                    
                        
                            "The transcriptional landscape of the mammalian genome." 
                        
                        
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                        ,
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                        ,
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                        ,
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                        ,
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                        ,
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                        ,
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                        ,
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                        ,
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                        ,
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                        ,
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                        ,
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                        ,
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                        ,
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                        ,
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                        ,
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                        ,
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                        ,
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                        ,
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                        ,
                            Fletcher C.F. 
                        ,
                            Fukushima T. 
                        ,
                            Furuno M. 
                        ,
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                        ,
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                        ,
                            Georgii-Hemming P. 
                        ,
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                        ,
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                            Ninomiya N. 
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                            Okada M. 
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                            Science309:1559-1563(2005)
                         
                        
                        
                        [
PubMed ]
                        [
Europe PMC ]
                        [
Abstract ]
                        
                    
 
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] 
                    
                    
                    strain :
                    
BALB/c . strain :
                    
C57BL/6J . strain :
                    
DBA/2 . tissue :
                    
Cerebellum . tissue :
                    
Eye . tissue :
                    
Head . tissue :
                    
Kidney . tissue :
                    
Lung .  
             
         
            
        
            
                3.
                
                    
                        
                            "Lineage-specific biology revealed by a finished genome assembly of the mouse." 
                        
                        
                            Church D.M. 
                        ,
                            
Goodstadt L. 
                        ,
                            
Hillier L.W. 
                        ,
                            
Zody M.C. 
                        ,
                            
Goldstein S. 
                        ,
                            
She X. 
                        ,
                            
Bult C.J. 
                        ,
                            
Agarwala R. 
                        ,
                            
Cherry J.L. 
                        ,
                            
DiCuccio M. 
                        ,
                            
Hlavina W. 
                        ,
                            
Kapustin Y. 
                        ,
                            
Meric P. 
                        ,
                            
Maglott D. 
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Birtle Z. 
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Marques A.C. 
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Graves T. 
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Zhou S. 
                        ,
                        
                        
                            Teague B. 
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                            Potamousis K. 
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                              more...   
                             
                        
                        
                        
                            PLoS Biol.7:E1000112-E1000112(2009)
                         
                        
                        
                        [
PubMed ]
                        [
Europe PMC ]
                        [
Abstract ]
                        
                    
 
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] 
                    
                    
                    strain :
                    
C57BL/6J .  
             
         
            
        
            
         
            
        
            
                5.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for PROTEIN SEQUENCE OF 4-11; 27-50; 65-78; 85-105; 116-137; 144-189; 199-213; 218-246 AND 262-333;IDENTIFICATION BY MASS SPECTROMETRY 
                    
                    
                    strain :
                    
C57BL/6 . strain :
                    
OF1 . tissue :
                    
Brain . tissue :
                    
Hippocampus .  
             
         
            
        
            
                6.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for NUCLEOTIDE SEQUENCE [MRNA] OF 24-249 
                    
                    
                 
             
         
            
        
            
                7.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for CATALYTIC ACTIVITY;SULFHYDRATION AT CYS-150;MUTAGENESIS OF CYS-150 
                    
                    
                 
             
         
            
        
            
                8.
                
                    
                    
                        [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s)
                        Cited for FUNCTION;SUBUNIT;RECONSTITUTION OF THE GAIT COMPLEX