Gapdh ELISA Kit for Rat(Rattus norvegicus) | EIAab.comGapdh ELISA Kit for Rat(Rattus norvegicus)

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Gapdh (Gene name),

Glyceraldehyde-3-phosphate dehydrogenase (Protein name ), G3P_RAT from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

Gapdh(Gapd);

Protein name:

Glyceraldehyde-3-phosphate dehydrogenase(GAPDH);

Alternative:

BARS-38;38 kDa BFA-dependent ADP-ribosylation substrate;2.6.99.-;Peptidyl-cysteine S-nitrosylase GAPDH);

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

Homotetramer. Interacts with EIF1AD, USP25, PRKCI and WARS. Interacts with TPPP; the interaction is direct (By similarity). Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation.

Function:

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.

Subcellular Location:

Cytoplasm cytosol Nucleus Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal.

Protein Attributes:

Sequence length:

333

Sequence:

50:

100:

150:

200:

250:

300:

333:

LNDNFVKLIS | WYDNEYGYSN | RVVDLMAYMA | SKE

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Rn.129558

String:

P04797

Pfam:

PF02800

KEGG:

rno:24383

SMR:

P04797

STRING:

10116.ENSRNOP00000040878

Uniprot:

P04797

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ELISA Kit for Rat GAPDH

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E1932m

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96T

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ELISA Kit for Rat GAPDH

Cat.:

E1932h

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96T

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ELISA Kit for Rat GAPDH

Cat.:

E1932d

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96T

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ELISA Kit for Rat GAPDH

Cat.:

E1932r

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96T

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ELISA Kit for Rat GAPDH

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ELISA Kit for Rat GAPDH

Cat.:

E1932p

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96T

ELISA Kit for Rat GAPDH

Cat.:

E1932b

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96T

ELISA Kit for Rat GAPDH

Cat.:

E1932c

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96T

CLIA Kit for Rat GAPDH

Cat.:

U1932b

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96T

CLIA Kit for Rat GAPDH

Cat.:

U1932d

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96T

CLIA Kit for Rat GAPDH

Cat.:

U1932m

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96T

CLIA Kit for Rat GAPDH

Cat.:

U1932p

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96T

CLIA Kit for Rat GAPDH

Cat.:

U1932c

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96T

CLIA Kit for Rat GAPDH

Cat.:

U1932r

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96T

CLIA Kit for Rat GAPDH

Cat.:

U1932h

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96T

CLIA Kit for Rat GAPDH

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Polyclonal Antibody for Rat GAPDH

Cat.:

P1932Rb-r

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40ug/0.2ml

Polyclonal Antibody for Rat GAPDH

Cat.:

P1932Rb-h

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40ug/0.2ml

Polyclonal Antibody for Rat GAPDH

Cat.:

P1932Rb-m

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40ug/0.2ml

Monoclonal Antibody for Rat GAPDH

Protein for Rat GAPDH

Cat.:

R1932h

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Packing:

1mg

Purity:

>90%

Protein for Rat GAPDH

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family."
Fort P. , Marty L. , Piechaczyk M. , el Sabrouty S. , Dani C. , Jeanteur P. , Blanchard J.-M.
Nucleic Acids Res.13:1431-1442(1985) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Wistar.
tissue: Muscle.
2.

"Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene."
Tso J.Y. , Sun X.-H. , Kao T.-H. , Reece K.S. , Wu R.
Nucleic Acids Res.13:2485-2502(1985) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Liver.
3.

"Over-expression of GAPDH induces apoptosis in COS-7 cells transfected with cloned GAPDH cDNAs."
Tajima H. , Tsuchiya K. , Yamada M. , Kondo K. , Katsube N. , Ishitani R.
NeuroReport10:2029-2033(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];FUNCTION
strain: Sprague-Dawley.
tissue: Cerebellum.
4.

"Isolation of a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) cDNA isoform from rat brain by a rapid PCR-based cloning method and its expression by RT-PCR."
Zheng J. , Ramirez V.D.
Submitted (2000-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Sprague-Dawley.
tissue: Brain.
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Brain.
tissue: Pituitary anterior lobe.
6.

Lubec G. , Afjehi-Sadat L. , Chen W.-Q.
Submitted (2007-04) to the UniProtKB

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 65-105; 116-137; 144-184; 233-246 AND 308-321;IDENTIFICATION BY MASS SPECTROMETRY
strain: Sprague-Dawley.
tissue: Hippocampus.
tissue: Spinal cord.
7.

"Trifluoperazine activates and releases latent ATP-generating enzymes associated with the synaptic plasma membrane."
Leung T.K.C. , Hall C. , Monfries C. , Lim L.
J. Neurochem.49:232-238(1987) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 235-333
tissue: Brain.
8.

"Post-transcriptional regulation of glyceraldehyde-3-phosphate-dehydrogenase gene expression in rat tissues."
Piechaczyk M. , Blanchard J.-M. , Marty L. , Dani C. , Panabieres F. , el Sabrouty S. , Fort P. , Jeanteur P.
Nucleic Acids Res.12:6951-6963(1984) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 261-323;TISSUE SPECIFICITY
tissue: Glial tumor.
9.

"1.5 kb mRNA abundantly expressed in rat tumors encodes a 37 kilodalton protein in vitro."
Maehara Y. , Fujiyoshi T. , Takahashi K. , Yamamoto M. , Endo H.
Biochem. Biophys. Res. Commun.131:800-805(1985) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 267-333
10.

"Nitric oxide-induced S-nitrosylation of glyceraldehyde-3-phosphate dehydrogenase inhibits enzymatic activity and increases endogenous ADP-ribosylation."
Molina y Vedia L. , McDonald B. , Reep B. , Brune B. , Di Silvio M. , Billiar T.R. , Lapetina E.G.
J. Biol. Chem.267:24929-24932(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: S-NITROSYLATION
11.

"Posttranslational modification of glyceraldehyde-3-phosphate dehydrogenase by S-nitrosylation and subsequent NADH attachment."
Mohr S. , Stamler J.S. , Brune B.
J. Biol. Chem.271:4209-4214(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: S-NITROSYLATION AT CYS-150;ADP-RIBOSYLATION AT CYS-150;ABSENCE OF S-NITROSYLATION AT CYS-154;ACTIVE SITE
12.

"Interactions among p22, glyceraldehyde-3-phosphate dehydrogenase and microtubules."
Andrade J. , Pearce S.T. , Zhao H. , Barroso M.
Biochem. J.384:327-336(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH CHP1;ASSOCIATION WITH MICROTUBULES;SUBCELLULAR LOCATION
13.

"S-nitrosylated GAPDH initiates apoptotic cell death by nuclear translocation following Siah1 binding."
Hara M.R. , Agrawal N. , Kim S.F. , Cascio M.B. , Fujimuro M. , Ozeki Y. , Takahashi M. , Cheah J.H. , Tankou S.K. , Hester L.D. , Ferris C.D. , Hayward S.D. , Snyder S.H. , Sawa A.
Nat. Cell Biol.7:665-674(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;INTERACTION WITH SIAH1;S-NITROSYLATION AT CYS-150;MUTAGENESIS OF CYS-150; LYS-225; PRO-234 AND THR-235
14.

"GOSPEL: a neuroprotective protein that binds to GAPDH upon S-nitrosylation."
Sen N. , Hara M.R. , Ahmad A.S. , Cascio M.B. , Kamiya A. , Ehmsen J.T. , Agrawal N. , Hester L. , Dore S. , Snyder S.H. , Sawa A.
Neuron63:81-91(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;INTERACTION WITH RILPL1 AND SIAH1
15.

"GAPDH mediates nitrosylation of nuclear proteins."
Kornberg M.D. , Sen N. , Hara M.R. , Juluri K.R. , Nguyen J.V. , Snowman A.M. , Law L. , Hester L.D. , Snyder S.H.
Nat. Cell Biol.12:1094-1100(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION AS NITROSYLASE;SUBCELLULAR LOCATION;INTERACTION WITH SIRT1;S-NITROSYLATION AT CYS-150;MUTAGENESIS OF SER-149; CYS-150; THR-151 AND THR-152

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