Glutathione peroxidase 2 (Protein name
), GPX2_HUMAN from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Gene name:
GPX2;
Protein name:
Glutathione peroxidase 2(GPx-2;GSHPx-2);
Alternative:
Glutathione peroxidase-gastrointestinal(GPx-GI;GSHPx-GI);Gastrointestinal glutathione peroxidase;Glutathione peroxidase-related protein 2(GPRP-2);
Organism:
Human (Homo sapiens).
General Annotation
Sub Unit:
Homotetramer.
Function:
Could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. Tert-butyl hydroperoxide, cumene hydroperoxide and linoleic acid hydroperoxide but not phosphatidycholine hydroperoxide, can act as acceptors.
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s
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤7.1%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤12.0%
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Recovery
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Recovery was determined by spiking various levels of Gpx2 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
103
99-105
Plasma
101
97-106
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gpx2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
99-105%
98-108%
95-109%
94-110%
EDTA plasma(n=5)
97-104%
97-103%
94-106%
96-107%
heparin plasma(n=5)
95-107%
93-108%
98-104%
95-105%
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT LEU-37
4.
NIEHS SNPs program
Submitted (2004-10) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS LEU-126 AND CYS-146
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Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
8.
"Crystal structure of the selenocysteine to cysteine mutant of human glutathione peroxidase 2 (GPX2)." Structural genomics consortium (SGC)
Submitted (2009-02) to the PDB data bank
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Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 4-188