Interferon gamma (Protein name
), IFNG_CANFA from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Gene name:
IFNG;
Protein name:
Interferon gamma(IFN-gamma);
Alternative:
Organism:
Dog (Canis familiarisCanis lupus familiaris).
General Annotation
Sub Unit:
Homodimer.
Function:
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
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s
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.6%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.9%
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Recovery
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Recovery was determined by spiking various levels of IFNG into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
98
96-100
Plasma
99
97-101
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFNG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
95-104%
96-100%
92-98%
102-105%
EDTA plasma(n=5)
89-95%
93-105%
98-104%
93-97%
heparin plasma(n=5)
96-103%
97-101%
99-105%
91-98%
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References
1.
"Cloning and expression of canine interferon-gamma." Boroughs K.L.
,
Sim G.-K.
Submitted (1999-02) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [MRNA]
Vaild Sequence:
