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Index > Protein center > IGF2(Gene name) > Pig
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  • IGF2 (Gene name),
  • Insulin-like growth factor II (Protein name ),  IGF2_PIG from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    IGF2;
    Protein name:
    Insulin-like growth factor II(IGF-II);
    Alternative:

    Organism:
    Pig (Sus scrofa). 
    General Annotation
    Sub Unit:
    N/A
    Function:
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
    Subcellular Location:
    Secreted
    Protein Attributes:
    Sequence length:
    181
    Sequence:
    50:
    MGIPMRKPLL | VLLVFLALAS | CCYAAYRPSE | TLCGGELVDT | LQFVCGDRGF | 
    100:
    YFSRPASRVN | RRSRGIVEEC | CFRSCDLALL | ETYCATPAKS | ERDVSTPPTV | 
    150:
    LPDNFPRYPV | GKFFRYDTWK | QSAQRLRRGL | PALLRARRGR | TLAKELEAVR | 
    181:
    EAKRHRPLTA | RPTRDPAAHG | GASPEASGHR | K
    3D Structure:
    N/A
    Predicted Eptitope:
    Please Sign in.
    EIAab Sequence  Vaild Sequence:
    Please Sign in.
    Related Databases
    UniGene:
    SMR:
    Pfam:
    Uniprot:
     
    FOR
    ELISA Kit for Pig IGF-II
    Cat.:
    E0051r
    Price:
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    MSDS:
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    96T
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    ELISA Kit for Pig IGF-II
    Cat.:
    E0051p
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Pig IGF-II
    Cat.:
    E0051m
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    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Pig IGF-II
    Cat.:
    E0051h
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    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    ELISA Kit for Pig IGF-II
    Cat.:
    E0051c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    ELISA Kit for Pig IGF-II
    Cat.:
    E0051b
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    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Pig IGF-II
    Cat.:
    U0051b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Pig IGF-II
    Cat.:
    U0051c
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Pig IGF-II
    Cat.:
    U0051h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Pig IGF-II
    Cat.:
    U0051m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Pig IGF-II
    Cat.:
    U0051p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Pig IGF-II
    Cat.:
    U0051r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    Polyclonal Antibody for Pig IGF-II
    Cat.:
    P0051Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Pig IGF-II
    Polyclonal Antibody for Pig IGF-II
    Cat.:
    P0051Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Pig IGF-II
    Polyclonal Antibody for Pig IGF-II
    Cat.:
    P0051Rb-h
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Pig IGF-II
    Monoclonal Antibody for Pig IGF-II
    Monoclonal Antibody for Pig IGF-II
    Monoclonal Antibody for Pig IGF-II
    Monoclonal Antibody for Pig IGF-II
    Monoclonal Antibody for Pig IGF-II
    Monoclonal Antibody for Pig IGF-II
    Protein for Pig IGF-II
    Protein for Pig IGF-II
    Protein for Pig IGF-II
    Protein for Pig IGF-II
    Protein for Pig IGF-II
    Protein for Pig IGF-II

    R&D Technical Data
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    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.8%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤6.7%

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    Recovery
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    Recovery was determined by spiking various levels of IGF2 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    91

    87-95

    Plasma

    92

    90-97

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    90-96%

    85-92%

    87-98%

    91-97%

    EDTA plasma(n=5)

    93-95%

    88-96%

    95-100%

    86-93%

    heparin plasma(n=5)

    88-94%

     

    91-98%

    100-106%

    80-87

     

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    References
    1. 1.
      "Nucleotide sequence of a porcine insulin-like growth factor II cDNA."
      Catchpole I.R. , Engstroem W.
      Nucleic Acids Res.18:6430-6430(1990) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      "Comparative sequence analysis of the INS-IGF2-H19 gene cluster in pigs."
      Amarger V. , Nguyen M. , Van Laere A.-S. , Braunschweig M. , Nezer C. , Georges M. , Andersson L.
      Mamm. Genome13:388-398(2002) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
      strain: Large white.
    3. 3.
      "A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig."
      Van Laere A.-S. , Nguyen M. , Braunschweig M. , Nezer C. , Collette C. , Moreau L. , Archibald A.L. , Haley C. , Buys N. , Tally M. , Andersson G. , Georges M. , Andersson L.
      Nature425:832-836(2003) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
      strain: European wild boar.
      strain: Hampshire.
      strain: Japanese wild boar.
      strain: Landrace.
      strain: Large white.
      strain: Meishan.
      strain: Pietrain.
    4. 4.
      "Purification, amino acid sequences and assay cross-reactivities of porcine insulin-like growth factor-I and -II."
      Francis G.L. , Owens P.C. , McNeil K.A. , Wallace J.C. , Ballard F.J.
      J. Endocrinol.122:681-687(1989) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 25-91
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