Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
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s
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s
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.4%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.5%
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.6%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.3%
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Recovery
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Recovery was determined by spiking various levels of IL-4 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
107
102-110
Plasma
108
105-112
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Recovery was determined by spiking various levels of IL-4 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
102
99-104
Plasma
100
97-103
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
103-107%
110-116%
104-110%
106-109%
EDTA plasma(n=5)
99-106%
105-110%
103-109%
106-112%
heparin plasma(n=5)
103-108%
107-113%
102-106%
110-115%
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
95-104%
91-96%
102-105%
97-101%
EDTA plasma(n=5)
96-103%
104-108%
97-100%
102-106%
heparin plasma(n=5)
93-98%
96-102%
99-107%
106-112%
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Vaild Sequence:
