IL8 ELISA Kit for Dog(Canis familiaris) | EIAab.comIL8 ELISA Kit for Dog(Canis familiaris)

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Index > Protein center > IL8(Gene name) > Dog

IL8 (Gene name),

Interleukin-8 (Protein name ), IL8_CANFA from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

IL8(CXCL8);

Protein name:

Interleukin-8(IL-8);

Alternative:

C-X-C motif chemokine 8;

Organism:

Dog (Canis familiarisCanis lupus familiaris).

General Annotation

Sub Unit:

Homodimer.

Function:

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

101

Sequence:

50:

100:

101:

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

STRING:

9615.ENSCAFP00000004516

Pfam:

PF00048

KEGG:

cfa:403850

UniGene:

Cfa.3510

SMR:

P41324

String:

P41324

Uniprot:

P41324

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ELISA Kit for Dog IL-8

Cat.:

E0080c

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96T

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ELISA Kit for Dog IL-8

Cat.:

E0080p

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96T

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ELISA Kit for Dog IL-8

Cat.:

E0080h

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ELISA Kit for Dog IL-8

MSDS:

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ELISA Kit for Dog IL-8

Cat.:

E0080d

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96T

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ELISA Kit for Dog IL-8

Cat.:

E0080b

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96T

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CLIA Kit for Dog IL-8

Cat.:

U0080b

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Packing:

96T

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CLIA Kit for Dog IL-8

Cat.:

U0080d

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Packing:

96T

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CLIA Kit for Dog IL-8

Cat.:

U0080c

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Manual:

Looking for manual.

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Packing:

96T

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Please sign in first.

CLIA Kit for Dog IL-8

Cat.:

U0080h

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Looking for manual.

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Packing:

96T

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Please sign in first.

CLIA Kit for Dog IL-8

Cat.:

U0080p

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Looking for manual.

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CLIA Kit for Dog IL-8

MSDS:

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Polyclonal Antibody for Dog IL-8

Cat.:

P0080Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Dog IL-8

Protein for Dog IL-8

Cat.:

R0080h

Price:

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Packing:

1mg

Purity:

>90%

Protein for Dog IL-8

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 6.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 8.6%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of IL8 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	100	97-106
Plasma	101	95-104

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	93-105
Plasma	98	94-106

Recovery was determined by spiking various levels of IL8 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	105	100-106
Plasma	104	102-107

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	100-105%	97-104%	95-101%	103-106%
EDTA plasma(n=5)	98-104%	94-103%	90-97%	105-109%
heparin plasma(n=5)	94-99%	103-108%	96-104%	98-105%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	91-103%	92-105%	95-106%	94-109%
EDTA plasma(n=5)	89-95%	93-103%	94-102%	95-106%
heparin plasma(n=5)	93-105%	94-107%	101-106%	96-108%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	98-102%	103-107%	97-105%	96-103%
EDTA plasma(n=5)	100-104%	99-107%	102-108%	97-102%
heparin plasma(n=5)	104-106%	94-102%	108-112%	97-103%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Cloning of a canine gene homologous to the human interleukin-8-encoding gene."
Ishikawa J. , Suzuki S. , Hotta K. , Hirota Y. , Mizuno S. , Suzuki K.
Gene131:305-306(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
2.

"Molecular cloning and expression of canine interleukin 8 cDNA."
Matsumoto Y. , Mohamed A. , Onodera T. , Kato H. , Ohashi T. , Goitsuka R. , Tsujimoto H. , Hasegawa A. , Furusawa S. , Yoshihara K. , Ishikawa J. , Hotta K. , Suzuki K. , Hirota Y.
Cytokine6:455-461(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Lymph node.
3.

"Interleukin-8 gene induction in the myocardium after ischemia and reperfusion in vivo."
Kukielka G.L. , Smith W.C. , Larosa G.J. , Manning A.M. , Mendoza L.H. , Daly T.J. , Hughes B.J. , Youker K.A. , Hawkins H.K. , Michael L.H. , Rot A. , Entman M.L.
J. Clin. Invest.95:89-103(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Mongrel.
tissue: Jugular vein.
4.

"Borrelia burgdorferi migrates into joint capsules and causes an up-regulation of interleukin-8 in synovial membranes of dogs experimentally infected with ticks."
Straubinger R.K. , Straubinger A.F. , Harter L. , Jacobson R.H. , Chang Y.-F. , Summers B.A. , Erb H.N. , Appel M.J.
Infect. Immun.65:1273-1285(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Beagle.

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