IL8 ELISA Kit for Human(Homo sapiens) | EIAab.comIL8 ELISA Kit for Human(Homo sapiens)

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Index > Protein center > IL8(Gene name) > Human

IL8 (Gene name),

Interleukin-8 (Protein name ), IL8_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

Citations (1)

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Gene name:

IL8(CXCL8);

Protein name:

Interleukin-8(IL-8);

Alternative:

Emoctakin;C-X-C motif chemokine 8;Monocyte-derived neutrophil chemotactic factor(MDNCF);Granulocyte chemotactic protein 1(GCP-1);Neutrophil-activating protein 1(NAP-1);Monocyte-derived neutrophil-activating peptide(MONAP);T-cell chemotactic factor;Protein 3-10C;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer.

Function:

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

Sequence:

50:

99:

ELRVIESGPH | CANTEIIVKL | SDGRELCLDP | KENWVQRVVE | KFLKRAENS

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

MIM:

146930

Pfam:

PF00048

UniGene:

Hs.624

KEGG:

hsa:3576

SMR:

P10145

String:

P10145

Uniprot:

P10145

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ELISA Kit for Human IL-8

Cat.:

E0080c

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96T

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ELISA Kit for Human IL-8

Cat.:

E0080p

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96T

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ELISA Kit for Human IL-8

Cat.:

E0080h

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96T

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ELISA Kit for Human IL-8

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ELISA Kit for Human IL-8

Cat.:

E0080d

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96T

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ELISA Kit for Human IL-8

Cat.:

E0080b

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96T

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CLIA Kit for Human IL-8

Cat.:

U0080b

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96T

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CLIA Kit for Human IL-8

Cat.:

U0080d

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96T

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CLIA Kit for Human IL-8

Cat.:

U0080c

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Looking for manual.

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Packing:

96T

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CLIA Kit for Human IL-8

Cat.:

U0080h

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Looking for manual.

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Packing:

96T

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CLIA Kit for Human IL-8

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U0080p

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CLIA Kit for Human IL-8

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Polyclonal Antibody for Human IL-8

Cat.:

P0080Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human IL-8

Protein for Human IL-8

Cat.:

R0080h

Price:

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Packing:

1mg

Purity:

>90%

Protein for Human IL-8

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

12% SDS-PAGE Analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 6.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 8.6%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of IL8 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	100	97-106
Plasma	101	95-104

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	93-105
Plasma	98	94-106

Recovery was determined by spiking various levels of IL8 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	105	100-106
Plasma	104	102-107

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	100-105%	97-104%	95-101%	103-106%
EDTA plasma(n=5)	98-104%	94-103%	90-97%	105-109%
heparin plasma(n=5)	94-99%	103-108%	96-104%	98-105%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	91-103%	92-105%	95-106%	94-109%
EDTA plasma(n=5)	89-95%	93-103%	94-102%	95-106%
heparin plasma(n=5)	93-105%	94-107%	101-106%	96-108%

Sample	1：2	1:4	1:8	1:16
serum(n=5)	98-102%	103-107%	97-105%	96-103%
EDTA plasma(n=5)	100-104%	99-107%	102-108%	97-102%
heparin plasma(n=5)	104-106%	94-102%	108-112%	97-103%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Citations

[1].

"Human-derived cathelicidin LL-37 directly activates mast cells to proinflammatory mediator synthesis and migratory response"

Bąbolewska E, Brzezińska-Błaszczyk E

From:Cellular Immunology

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[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
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J. Immunol.143:1366-1371(1989) [PubMed] [Europe PMC] [Abstract]

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Mol. Cell. Biol.9:1946-1957(1989) [PubMed] [Europe PMC] [Abstract]

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"Coding region structure of interleukin-8 gene of human lung giant cell carcinoma LU65C cells that produce LUCT/interleukin-8: homogeneity in interleukin-8 genes."
Hotta K. , Hayashi K. , Ishikawa J. , Tagawa M. , Hashimoto K. , Mizuno S. , Suzuki K.
Immunol. Lett.24:165-169(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
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Jang J.S. , Kim B.E.
Submitted (1998-02) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)
tissue: Peripheral blood leukocyte.
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"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue: Neutrophil.
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"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
Submitted (2003-05) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
9.

"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Halleck A. , Ebert L. , Mkoundinya M. , Schick M. , Eisenstein S. , Neubert P. , Kstrang K. , Schatten R. , Shen B. , Henze S. , Mar W. , Korn B. , Zuo D. , Hu Y. , LaBaer J.
Submitted (2004-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
10.

SeattleSNPs variation discovery resource
Submitted (2001-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
11.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
12.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue: Lung.
13.

"cDNA cloning of human mesangial cell interleukin 8 by polymerase chain reaction."
King C.H. , Gordon G.S. , Konieczkowski M. , Sedor J.R.
Submitted (1992-02) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-97 (ISOFORM 1)
tissue: Kidney.
14.

"Purification of granulocyte chemotactic peptide/interleukin-8 reveals N-terminal sequence heterogeneity similar to that of beta-thromboglobulin."
van Damme J. , van Beeumen J. , Conings R. , Decock B. , Billiau A.
Eur. J. Biochem.181:337-344(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-41;IDENTIFICATION OF MDNCF-A; IL-8(1-77); IL-8(6-77); IL-8(7-77); IL-8(8-77) AND IL-8(9-77);PROTEOLYTIC PROCESSING OF N-TERMINUS
15.

"The neutrophil-activating proteins interleukin 8 and beta-thromboglobulin: in vitro and in vivo comparison of NH2-terminally processed forms."
van Damme J. , Rampart M. , Coning R. , Decock B. , van Osselaer N. , Willems J. , Billiau A.
Eur. J. Immunol.20:2113-2118(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-41;IDENTIFICATION OF MDNCF-A; IL-8(1-77); IL-8(6-77); IL-8(7-77); IL-8(8-77) AND IL-8(9-77);PROTEOLYTIC PROCESSING OF N-TERMINUS;FUNCTION
16.

"Three forms of monocyte-derived neutrophil chemotactic factor (MDNCF) distinguished by different lengths of the amino-terminal sequence."
Yoshimura T. , Robinson E.A. , Appella E. , Matsushima K. , Showalter S.D. , Skeel A. , Leonard E.J.
Mol. Immunol.26:87-93(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-32;IDENTIFICATION OF MDNCF-A; IL-8(1-77) AND IL-8(6-77);PROTEOLYTIC PROCESSING OF N-TERMINUS
17.

"Purification and partial primary sequence of a chemotactic protein for polymorphonuclear leukocytes derived from human lung giant cell carcinoma LU65C cells."
Suzuki K. , Miyasaka H. , Ota H. , Yamakawa Y. , Tagawa M. , Kuramoto A. , Mizuno S.
J. Exp. Med.169:1895-1901(1989) [PubMed] [Europe PMC] [Abstract]

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"Biochemical and biological characterization of NAP-1/IL-8-related cytokines in lesional psoriatic scale."
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Golds E.E. , Mason P. , Nyirkos P.
Biochem. J.259:585-588(1989) [PubMed] [Europe PMC] [Abstract]

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"Isolation and amino acid sequence of a chemotactic protein, LECT/interleukin 8, from a human myeloid leukemia cell line, ML-1."
Suzuki K. , Yamakawa Y. , Matsuo Y. , Kamiya T. , Minowada J. , Mizuno S.
Immunol. Lett.36:71-81(1993) [PubMed] [Europe PMC] [Abstract]

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Gregory H. , Young J. , Schroeder J.-M. , Mrowietz U. , Christophers E.
Biochem. Biophys. Res. Commun.151:883-890(1988) [PubMed] [Europe PMC] [Abstract]

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22.

"Purification of a human monocyte-derived neutrophil chemotactic factor that has peptide sequence similarity to other host defense cytokines."
Yoshimura T. , Matsushima K. , Tanaka S. , Robinson E.A. , Appella E. , Oppenheim J.J. , Leonard E.J.
Proc. Natl. Acad. Sci. U.S.A.84:9233-9237(1987) [PubMed] [Europe PMC] [Abstract]

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"Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes."
Walz A. , Peveri P. , Aschauer H. , Baggiolini M.
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"Endothelial and leukocyte forms of IL-8. Conversion by thrombin and interactions with neutrophils."
Hebert C.A. , Luscinskas F.W. , Kiely J.-M. , Luis E.A. , Darbonne W.C. , Bennett G.L. , Liu C.C. , Obin M.S. , Gimbrone M.A. Jr. , Baker J.B.
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Clark-Lewis I. , Mose B. , Walz A. , Baggiolini M. , Scott G.J. , Aebersold R.
Biochemistry30:3128-3135(1991) [PubMed] [Europe PMC] [Abstract]

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"Neutrophil gelatinase B potentiates interleukin-8 tenfold by aminoterminal processing, whereas it degrades CTAP-III, PF-4, and GRO-alpha and leaves RANTES and MCP-2 intact."
Van den Steen P.E. , Proost P. , Wuyts A. , Van Damme J. , Opdenakker G.
Blood96:2673-2681(2000) [PubMed] [Europe PMC] [Abstract]

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"Identification of biologically active chemokine isoforms from ascitic fluid and elevated levels of CCL18/pulmonary and activation-regulated chemokine in ovarian carcinoma."
Schutyser E. , Struyf S. , Proost P. , Opdenakker G. , Laureys G. , Verhasselt B. , Peperstraete L. , Van de Putte I. , Saccani A. , Allavena P. , Mantovani A. , Van Damme J.
J. Biol. Chem.277:24584-24593(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION OF IL-8(5-77) AND IL-8(6-77);FUNCTION;PROTEOLYTIC PROCESSING OF N-TERMINUS
28.

"Interleukin-8, a chemotactic and inflammatory cytokine."
Baggiolini M. , Clark-Lewis I.
FEBS Lett.307:97-101(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
29.

"Regulation of the immune response by the interaction of chemokines and proteases."
Struyf S. , Proost P. , Van Damme J.
Adv. Immunol.81:1-44(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
30.

"Citrullination of CXCL8 by peptidylarginine deiminase alters receptor usage, prevents proteolysis, and dampens tissue inflammation."
Proost P. , Loos T. , Mortier A. , Schutyser E. , Gouwy M. , Noppen S. , Dillen C. , Ronsse I. , Conings R. , Struyf S. , Opdenakker G. , Maudgal P.C. , Van Damme J.
J. Exp. Med.205:2085-2097(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CITRULLINATION AT ARG-27
31.

"Citrullination of CXCL8 increases this chemokine's ability to mobilize neutrophils into the blood circulation."
Loos T. , Opdenakker G. , Van Damme J. , Proost P.
Haematologica94:1346-1353(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CITRULLINATION AT ARG-27
32.

"Endoplasmic reticulum stress-activated C/EBP homologous protein enhances nuclear factor-kappaB signals via repression of peroxisome proliferator-activated receptor gamma."
Park S.H. , Choi H.J. , Yang H. , Do K.H. , Kim J. , Lee D.W. , Moon Y.
J. Biol. Chem.285:35330-35339(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INDUCTION
33.

"Determination of the secondary structure of interleukin-8 by nuclear magnetic resonance spectroscopy."
Clore G.M. , Appella E. , Yamada M. , Matsushima K. , Gronenborn A.M.
J. Biol. Chem.264:18907-18911(1989) [PubMed] [Europe PMC] [Abstract]

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34.

"Three-dimensional structure of interleukin 8 in solution."
Clore G.M. , Appella E. , Yamada M. , Matsushima K. , Gronenborn A.M.
Biochemistry29:1689-1696(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR
35.

"Structure and activity of a chimeric interleukin-8-melanoma-growth-stimulatory-activity protein."
Sticht H. , Auer M. , Schmitt B. , Besemer J. , Horcher M. , Kirsch T. , Lindley I.J. , Rosch P.
Eur. J. Biochem.235:26-35(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 28-80
36.

"Structure of a CXC chemokine-receptor fragment in complex with interleukin-8."
Skelton N.J. , Quan C. , Reilly D. , Lowman H.
Structure7:157-168(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF COMPLEX TO RECEPTOR
37.

"Crystallization of human interleukin-8. A protein chemotactic for neutrophils and T-lymphocytes."
Baldwin E.T. , Franklin K.A. , Appella E. , Yamada M. , Matsushima K. , Wlodawer A. , Weber I.T.
J. Biol. Chem.265:6851-6853(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS)
38.

"Comparison of the solution nuclear magnetic resonance and crystal structures of interleukin-8. Possible implications for the mechanism of receptor binding."
Clore G.M. , Gronenborn A.M.
J. Mol. Biol.217:611-620(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR;COMPARISON WITH X-RAY STRUCTURE
39.

"Crystal structure of interleukin 8: symbiosis of NMR and crystallography."
Baldwin E.T. , Weber I.T. , St Charles R. , Xuan J.C. , Appella E. , Yamada M. , Matsushima K. , Edwards B.F. , Clore G.M. , Gronenborn A.M.
Proc. Natl. Acad. Sci. U.S.A.88:502-506(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS);STRUCTURE BY NMR
40.

"Receptor-binding conformation of the 'ELR' motif of IL-8: X-ray structure of the L5C/H33C variant at 2.35 A resolution."
Gerber N. , Lowman H. , Artis D.R. , Eigenbrot C.
Proteins38:361-367(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF MUTANT

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