KPNA1 ELISA Kit for Human(Homo sapiens) | EIAab.comKPNA1 ELISA Kit for Human(Homo sapiens)

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KPNA1 (Gene name),

Importin subunit alpha-1 (Protein name ), IMA1_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

KPNA1(RCH2;SRP1);

Protein name:

Importin subunit alpha-1;

Alternative:

Nucleoprotein interactor 1(NPI-1);Karyopherin subunit alpha-1;SRP1-beta;RAG cohort protein 2;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Forms a complex with importin subunit beta-1. Interacts with ANP32E. Interacts with ZIC3 (By similarity). Interacts with the nucleoprotein of influenza A viruses. Binds to HCMV (human cytomegalovirus) UL84, HIV-1 Vpr and to ebolavirus VP24. Interacts with APEX1.

Function:

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.

Subcellular Location:

Cytoplasm Nucleus

Protein Attributes:

Sequence length:

538

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

538:

EHYFGTEDED | SSIAPQVDLN | QQQYIFQQCE | APMEGFQL

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

hsa:3836

UniGene:

Hs.161008

SMR:

P52294

Pfam:

PF00514

STRING:

9606.ENSP00000332455

KEGG:

hsa:3838

MIM:

600685

String:

P52294

MIM:

600686

UniGene:

Hs.594238

SMR:

P52292

Uniprot:

P52294

FOR

ELISA Kit for Human Importin subunit alpha-1

CLIA Kit for Human Importin subunit alpha-1

Polyclonal Antibody for Human Importin subunit alpha-1

Monoclonal Antibody for Human Importin subunit alpha-1

Protein for Human Importin subunit alpha-1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Identification of hSRP1 alpha as a functional receptor for nuclear localization sequences."
Weis K. , Mattaj I.W. , Lamond A.I.
Science268:1049-1053(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"Genomic structure of karyopherin alpha2 (KPNA2) within a low-copy repeat on chromosome 17q23-q24 and mutation analysis in patients with Russell-Silver syndrome."
Doerr S. , Schlicker M. , Hansmann I.
Hum. Genet.109:479-486(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT ARG-165
3.

"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
Submitted (2003-05) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANTS VAL-157 AND ASN-453
4.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA];VARIANTS VAL-157 AND ASN-453
tissue: Bone marrow.
tissue: Testis.
6.

Bienvenut W.V. , Boldt K. , von Kriegsheim A.F. , Kolch W.
Submitted (2007-07) to the UniProtKB

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-13; 69-101; 107-117; 239-258; 292-315; 354-388 AND 487-494;CLEAVAGE OF INITIATOR METHIONINE;ACETYLATION AT SER-2;IDENTIFICATION BY MASS SPECTROMETRY
tissue: Hepatoma.
7.

"Rch1, a protein that specifically interacts with the RAG-1 recombination-activating protein."
Cuomo C.A. , Kirch S.A. , Gyuris J. , Brent R. , Oettinger M.A.
Proc. Natl. Acad. Sci. U.S.A.91:6156-6160(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-529
tissue: Cervix carcinoma.
8.

"Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal and beta subunit interacts with peptide repeat-containing nucleoporins."
Moroianu J. , Hijikata M. , Blobel G. , Radu A.
Proc. Natl. Acad. Sci. U.S.A.92:6532-6536(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBUNIT;SUBCELLULAR LOCATION
9.

"The conserved amino-terminal domain of hSRP1 alpha is essential for nuclear protein import."
Weis K. , Ryder U. , Lamond A.I.
EMBO J.15:1818-1825(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DOMAIN IBB
10.

"Export of importin-alpha from the nucleus is mediated by a specific nuclear transport factor."
Kutay U. , Bischoff F.R. , Kostka S. , Kraft R. , Goerlich D.
Cell90:1061-1071(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION IN A COMPLEX WITH RAN AND CSE1L
11.

"Viral protein R regulates nuclear import of the HIV-1 pre-integration complex."
Popov S. , Rexach M. , Zybarth G. , Reiling N. , Lee M.A. , Ratner L. , Lane C.M. , Moore M.S. , Blobel G. , Bukrinsky M.
EMBO J.17:909-917(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HIV-1 VPR
12.

"Determination of the functional domain organization of the importin alpha nuclear import factor."
Herold A. , Truant R. , Wiegand H. , Cullen B.R.
J. Cell Biol.143:309-318(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH XPO2/CSE1L
13.

"ARL4, an ARF-like protein that is developmentally regulated and localized to nuclei and nucleoli."
Lin C.Y. , Huang P.H. , Liao W.L. , Cheng H.J. , Huang C.F. , Kuo J.C. , Patton W.A. , Massenburg D. , Moss J. , Lee F.J.
J. Biol. Chem.275:37815-37823(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ARL4A
14.

"Identification of a karyopherin alpha 2 recognition site in PLAG1, which functions as a nuclear localization signal."
Braem C.V. , Kas K. , Meyen E. , Debiec-Rychter M. , Van De Ven W.J.M. , Voz M.L.
J. Biol. Chem.277:19673-19678(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PLAG1
15.

"Cluster analysis of an extensive human breast cancer cell line protein expression map database."
Harris R.A. , Yang A. , Stein R.C. , Lucy K. , Brusten L. , Herath A. , Parekh R. , Waterfield M.D. , O'Hare M.J. , Neville M.A. , Page M.J. , Zvelebil M.J.
Proteomics2:212-223(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MASS SPECTROMETRY
tissue: Mammary cancer.
16.

"Importin KPNA2 is required for proper nuclear localization and multiple functions of NBS1."
Tseng S.-F. , Chang C.-Y. , Wu K.-J. , Teng S.-C.
J. Biol. Chem.280:39594-39600(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH NBN
17.

"Analysis of nuclear transport signals in the human apurinic/apyrimidinic endonuclease (APE1/Ref1)."
Jackson E.B. , Theriot C.A. , Chattopadhyay R. , Mitra S. , Izumi T.
Nucleic Acids Res.33:3303-3312(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH APEX1
18.

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
19.

"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A. , Villen J. , Gerber S.A. , Rush J. , Gygi S.P.
Nat. Biotechnol.24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
20.

"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T. , Yi W. , Lu B. , Park S.K. , Xu T. , Lee J.-D. , Yates J.R. III
J. Proteome Res.7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
21.

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H. , Olsen J.V. , Bairlein M. , Gnad F. , Oppermann F.S. , Korner R. , Greff Z. , Keri G. , Stemmann O. , Mann M.
Mol. Cell31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
22.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62 AND SER-490;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
23.

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S. , Helbig A.O. , Slijper M. , Krijgsveld J. , Heck A.J. , Mohammed S.
Anal. Chem.81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
24.

"Characterization of Snail nuclear import pathways as representatives of C2H2 zinc finger transcription factors."
Mingot J.M. , Vega S. , Maestro B. , Sanz J.M. , Nieto M.A.
J. Cell Sci.122:1452-1460(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SNAI1 AND SNAI2
25.

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Leukemic T-cell.
26.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62 AND SER-490;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
27.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
28.

"Importin alpha protein acts as a negative regulator for Snail protein nuclear import."
Sekimoto T. , Miyamoto Y. , Arai S. , Yoneda Y.
J. Biol. Chem.286:15126-15131(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SNAI1
29.

"CTNNBL1 is a novel nuclear localization sequence-binding protein that recognizes RNA-splicing factors CDC5L and Prp31."
Ganesh K. , Adam S. , Taylor B. , Simpson P. , Rada C. , Neuberger M.
J. Biol. Chem.286:17091-17102(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH CTNNBL1
30.

"Facioscapulohumeral muscular dystrophy region gene 1 is a dynamic RNA-associated and actin-bundling protein."
Sun C.Y. , van Koningsbruggen S. , Long S.W. , Straasheijm K. , Klooster R. , Jones T.I. , Bellini M. , Levesque L. , Brieher W.M. , van der Maarel S.M. , Jones P.L.
J. Mol. Biol.411:397-416(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH FRG1
31.

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2;PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
32.

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P. , Lasa M. , Polevoda B. , Gazquez C. , Elosegui-Artola A. , Kim D.S. , De Juan-Pardo E. , Demeyer K. , Hole K. , Larrea E. , Timmerman E. , Prieto J. , Arnesen T. , Sherman F. , Gevaert K. , Aldabe R.
Proc. Natl. Acad. Sci. U.S.A.109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
33.

"Structure of importin-beta bound to the IBB domain of importin-alpha."
Cingolani G. , Petosa C. , Weis K. , Muller C.W.
Nature399:221-229(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 11-54

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