IRF3 ELISA Kit for Human(Homo sapiens) | EIAab.comIRF3 ELISA Kit for Human(Homo sapiens)

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IRF3 (Gene name),

Interferon regulatory factor 3 (Protein name ), IRF3_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

IRF3;

Protein name:

Interferon regulatory factor 3(IRF-3);

Alternative:

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer; phosphorylation-induced. Interacts with CREBBP. May interact with MAVS. Interacts with IKBKE and TBK1. Interacts with TICAM1 and TICAM2. Interacts with rotavirus A NSP1 (via C-terminus); this interaction leads to the proteasome-dependent degradation of IRF3. Interacts with RBCK1. Interacts with TRIM21. Interacts with HERC5.

Function:

Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.

Subcellular Location:

Cytoplasm Nucleus Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.

Protein Attributes:

Sequence length:

427

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

427:

LSLTSDQYKA | YLQDLVEGMD | FQGPGES

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF00605

STRING:

9606.ENSP00000310127

UniGene:

Hs.289052

MIM:

603734

KEGG:

hsa:3661

String:

Q14653

SMR:

Q14653

Uniprot:

Q14653

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ELISA Kit for Human IRF-3

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E1491m

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96T

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ELISA Kit for Human IRF-3

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E1491h

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96T

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ELISA Kit for Human IRF-3

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E1491b

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96T

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ELISA Kit for Human IRF-3

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E1491c

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ELISA Kit for Human IRF-3

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E1491p

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96T

CLIA Kit for Human IRF-3

Cat.:

U1491h

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96T

CLIA Kit for Human IRF-3

Cat.:

U1491m

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96T

CLIA Kit for Human IRF-3

Cat.:

U1491b

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96T

CLIA Kit for Human IRF-3

Cat.:

U1491c

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96T

CLIA Kit for Human IRF-3

Cat.:

U1491p

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96T

Polyclonal Antibody for Human IRF-3

Cat.:

P1491Rb-h

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40ug/0.2ml

Polyclonal Antibody for Human IRF-3

Cat.:

P1491Rb-m

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40ug/0.2ml

Polyclonal Antibody for Human IRF-3

Monoclonal Antibody for Human IRF-3

Protein for Human IRF-3

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes."
Au W.W.-C. , Moore P.P.A. , Lowther W.W. , Juang Y.-T. , Pitha P.M.
Proc. Natl. Acad. Sci. U.S.A.92:11657-11661(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
tissue: Retina.
2.

"IRF3 mRNA, nirs splice variants."
Tabata Y. , Sameshima E. , Hayashi A. , Iida K. , Mitsuyama M. , Kanai S. , Furuya T. , Saito T.
Submitted (2003-02) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 5)
3.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1);VARIANT THR-427
tissue: Spleen.
4.

"The DNA sequence and biology of human chromosome 19."
Grimwood J. , Gordon L.A. , Olsen A.S. , Terry A. , Schmutz J. , Lamerdin J.E. , Hellsten U. , Goodstein D. , Couronne O. , Tran-Gyamfi M. , Aerts A. , Altherr M. , Ashworth L. , Bajorek E. , Black S. , Branscomb E. , Caenepeel S. , Carrano A.V. , more...
Nature428:529-535(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA];VARIANT THR-427
6.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4)
tissue: Eye.
tissue: Kidney.
7.

"Mapping of human interferon regulatory factor 3 (IRF3) to chromosome 19q13.3-13.4 by an intragenic polymorphic marker."
Bellingham J. , Gregory-Evans K. , Gregory-Evans C.Y.
Ann. Hum. Genet.62:231-234(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 323-413
8.

"Virus-dependent phosphorylation of the IRF-3 transcription factor regulates nuclear translocation, transactivation potential, and proteasome-mediated degradation."
Lin R. , Heylbroeck C. , Pitha P.M. , Hiscott J.
Mol. Cell. Biol.18:2986-2996(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF 385-SER-SER-386 AND 396-SER--SER-405
9.

"Analyses of virus-induced homomeric and heteromeric protein associations between IRF-3 and coactivator CBP/p300."
Suhara W. , Yoneyama M. , Iwamura T. , Yoshimura S. , Tamura K. , Namiki H. , Aimoto S. , Fujita T.
J. Biochem.128:301-307(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF SER-385 AND SER-386
10.

"Regulated nuclear-cytoplasmic localization of interferon regulatory factor 3, a subunit of double-stranded RNA-activated factor 1."
Kumar K.P. , McBride K.M. , Weaver B.K. , Dingwall C. , Reich N.C.
Mol. Cell. Biol.20:4159-4168(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;MUTAGENESIS OF 77-LYS-ARG-78; 86-ARG-LYS-87 AND 139-ILE-LEU-140
11.

"Identification of distinct signaling pathways leading to the phosphorylation of interferon regulatory factor 3."
Servant M.J. , ten Oever B. , LePage C. , Conti L. , Gessani S. , Julkunen I. , Lin R. , Hiscott J.
J. Biol. Chem.276:355-363(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION
12.

"IRF3 and IRF7 phosphorylation in virus-infected cells does not require double-stranded RNA-dependent protein kinase R or Ikappa B kinase but is blocked by Vaccinia virus E3L protein."
Smith E.J. , Marie I.J. , Prakash A. , Garcia-Sastre A. , Levy D.E.
J. Biol. Chem.276:8951-8957(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INHIBITION OF PHOSPHORYLATION BY VACCINIA VIRUS PROTEIN E3
13.

"HHV-8 encoded vIRF-1 represses the interferon antiviral response by blocking IRF-3 recruitment of the CBP/p300 coactivators."
Lin R. , Genin P. , Mamane Y. , Sgarbanti M. , Battistini A. , Harrington W.J. Jr. , Barber G.N. , Hiscott J.
Oncogene20:800-811(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HHV-8 PROTEIN VIRF1
14.

"Control of IRF-3 activation by phosphorylation."
Yoneyama M. , Suhara W. , Fujita T.
J. Interferon Cytokine Res.22:73-76(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
15.

"A novel Toll/IL-1 receptor domain-containing adapter that preferentially activates the IFN-beta promoter in the Toll-like receptor signaling."
Yamamoto M. , Sato S. , Mori K. , Hoshino K. , Takeuchi O. , Takeda K. , Akira S.
J. Immunol.169:6668-6672(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TICAM1
16.

"Interferon regulatory factor 3 is a cellular partner of rotavirus NSP1."
Graff J.W. , Mitzel D.N. , Weisend C.M. , Flenniken M.L. , Hardy M.E.
J. Virol.76:9545-9550(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ROTAVIRUS A NSP1
17.

"LPS-TLR4 signaling to IRF-3/7 and NF-kappaB involves the toll adapters TRAM and TRIF."
Fitzgerald K.A. , Rowe D.C. , Barnes B.J. , Caffrey D.R. , Visintin A. , Latz E. , Monks B. , Pitha P.M. , Golenbock D.T.
J. Exp. Med.198:1043-1055(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TICAM2
18.

Fitzgerald K.A. , Rowe D.C. , Barnes B.J. , Caffrey D.R. , Visintin A. , Latz E. , Monks B. , Pitha P.M. , Golenbock D.T.
J. Exp. Med.198:1451-1451(2003)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
19.

"Triggering the interferon antiviral response through an IKK-related pathway."
Sharma S. , tenOever B.R. , Grandvaux N. , Zhou G.-P. , Lin R. , Hiscott J.
Science300:1148-1151(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION
20.

"Mechanisms of the TRIF-induced interferon-stimulated response element and NF-kappaB activation and apoptosis pathways."
Han K.J. , Su X. , Xu L.-G. , Bin L.H. , Zhang J. , Shu H.-B.
J. Biol. Chem.279:15652-15661(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TICAM1
21.

"SIKE is an IKK epsilon/TBK1-associated suppressor of TLR3- and virus-triggered IRF-3 activation pathways."
Huang J. , Liu T. , Xu L.-G. , Chen D. , Zhai Z. , Shu H.-B.
EMBO J.24:4018-4028(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH IKBKE AND TBK1
22.

"VISA is an adapter protein required for virus-triggered IFN-beta Signaling."
Xu L.-G. , Wang Y.-Y. , Han K.-J. , Li L.-Y. , Zhai Z. , Shu H.-B.
Mol. Cell19:727-740(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MAVS
23.

"Distinct functions of IRF-3 and IRF-7 in IFN-alpha gene regulation and control of anti-tumor activity in primary macrophages."
Solis M. , Goubau D. , Romieu-Mourez R. , Genin P. , Civas A. , Hiscott J.
Biochem. Pharmacol.72:1469-1476(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION
24.

"Type I interferon gene induction by the interferon regulatory factor family of transcription factors."
Honda K. , Takaoka A. , Taniguchi T.
Immunity25:349-360(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION
25.

Honda K. , Takaoka A. , Taniguchi T.
Immunity25:849-849(2006)

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ERRATUM
26.

"Negative feedback regulation of cellular antiviral signaling by RBCK1-mediated degradation of IRF3."
Zhang M. , Tian Y. , Wang R.P. , Gao D. , Zhang Y. , Diao F.C. , Chen D.Y. , Zhai Z.H. , Shu H.B.
Cell Res.18:1096-1104(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION;INTERACTION WITH RBCK1
27.

"The E3 ubiquitin ligase Ro52 negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3."
Higgs R. , Ni Gabhann J. , Ben Larbi N. , Breen E.P. , Fitzgerald K.A. , Jefferies C.A.
J. Immunol.181:1780-1786(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TRIM21;POLYUBIQUITINATION
28.

"Ebola virus protein VP35 impairs the function of interferon regulatory factor-activating kinases IKKepsilon and TBK-1."
Prins K.C. , Cardenas W.B. , Basler C.F.
J. Virol.83:3069-3077(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION BY IKBKE AND TBK1
29.

"Regulation of immunity and oncogenesis by the IRF transcription factor family."
Savitsky D. , Tamura T. , Yanai H. , Taniguchi T.
Cancer Immunol. Immunother.59:489-510(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW ON FUNCTION
30.

"Positive regulation of interferon regulatory factor 3 activation by Herc5 via ISG15 modification."
Shi H.X. , Yang K. , Liu X. , Liu X.Y. , Wei B. , Shan Y.F. , Zhu L.H. , Wang C.
Mol. Cell. Biol.30:2424-2436(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ISGYLATION AT LYS-193; LYS-360 AND LYS-366;MUTAGENESIS OF LYS-193; LYS-360 AND LYS-366;INTERACTION WITH HERC5
31.

"Human DEAD box helicase 3 couples IkappaB kinase epsilon to interferon regulatory factor 3 activation."
Gu L. , Fullam A. , Brennan R. , Schroder M.
Mol. Cell. Biol.33:2004-2015(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH DDX3X AND IKBKE;PHOSPHORYLATION AT SER-396
32.

"Structural insights into the functions of TBK1 in innate antimicrobial immunity."
Shu C. , Sankaran B. , Chaton C.T. , Herr A.B. , Mishra A. , Peng J. , Li P.
Structure21:1137-1148(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-3; SER-14; THR-75; SER-97; THR-180; SER-188; THR-237; THR-244; THR-253; SER-398; THR-404 AND SER-427;PHOSPHORYLATION AT SER-386 BY TBK1;MUTAGENESIS OF SER-386
33.

"X-ray crystal structure of IRF-3 and its functional implications."
Takahasi K. , Suzuki N.N. , Horiuchi M. , Mori M. , Suhara W. , Okabe Y. , Fukuhara Y. , Terasawa H. , Akira S. , Fujita T. , Inagaki F.
Nat. Struct. Biol.10:922-927(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 175-427;DISULFIDE BOND
34.

"Crystal structure of IRF-3 reveals mechanism of autoinhibition and virus-induced phosphoactivation."
Qin B.Y. , Liu C. , Lam S.S. , Srinath H. , Delston R. , Correia J.J. , Derynck R. , Lin K.
Nat. Struct. Biol.10:913-921(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 173-427;DISULFIDE BOND
35.

"Crystal structure of ATF-2/c-Jun and IRF-3 bound to the interferon-beta enhancer."
Panne D. , Maniatis T. , Harrison S.C.
EMBO J.23:4384-4393(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 1-112
36.

"Crystal structure of IRF-3 in complex with CBP."
Qin B.Y. , Liu C. , Srinath H. , Lam S.S. , Correia J.J. , Derynck R. , Lin K.
Structure13:1269-1277(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 173-394

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