IRS1 ELISA Kit for Human(Homo sapiens) | EIAab.comIRS1 ELISA Kit for Human(Homo sapiens)

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IRS1 (Gene name),

Insulin receptor substrate 1 (Protein name ), IRS1_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

IRS1;

Protein name:

Insulin receptor substrate 1(IRS-1);

Alternative:

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Interacts with UBTF and PIK3CA (By similarity). Interacts (via phosphorylated YXXM motifs) with PIK3R1 (By similarity). Interacts with ROCK1 and FER (By similarity). Interacts (via PH domain) with PHIP (By similarity). Interacts with GRB2 (By similarity). Interacts with SOCS7. Interacts (via IRS-type PTB domain) with IGF1R and INSR (via the tyrosine-phosphorylated NPXY motif). Interacts with ALK.

Function:

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.

Subcellular Location:

N/A

Protein Attributes:

Sequence length:

1242

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

750:

800:

850:

900:

950:

1000:

1050:

1100:

1150:

1200:

1242:

PPPPPPPHQP | LGSGESSSTR | RSSEDLSAYA | SISFQKQPED | RQ

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

STRING:

9606.ENSP00000304895

SMR:

P35568

UniGene:

Hs.471508

MIM:

125853

KEGG:

hsa:3667

Pfam:

PF02174

String:

P35568

Uniprot:

P35568

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ELISA Kit for Human IRS-1

Cat.:

E1660h

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96T

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ELISA Kit for Human IRS-1

Cat.:

E1660m

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96T

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ELISA Kit for Human IRS-1

Cat.:

E1660r

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96T

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CLIA Kit for Human IRS-1

Cat.:

U1660r

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Looking for manual.

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Packing:

96T

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CLIA Kit for Human IRS-1

Cat.:

U1660m

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MSDS:

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Packing:

96T

CLIA Kit for Human IRS-1

Cat.:

U1660h

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Packing:

96T

Polyclonal Antibody for Human IRS-1

Cat.:

P1660Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human IRS-1

Cat.:

P1660Rb-h

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40ug/0.2ml

Polyclonal Antibody for Human IRS-1

Cat.:

P1660Rb-r

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human IRS-1

Protein for Human IRS-1

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Human skeletal muscle insulin receptor substrate-1. Characterization of the cDNA, gene, and chromosomal localization."
Araki E. , Sun X.J. , Haag B.L. III , Chuang L.M. , Zhang Y. , Yang-Feng T.L. , White M.F. , Kahn C.R.
Diabetes42:1041-1054(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Skeletal muscle.
2.

"Cloning and increased expression of an insulin receptor substrate-1-like gene in human hepatocellular carcinoma."
Nishiyama M. , Wands J.R.
Biochem. Biophys. Res. Commun.183:280-285(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
3.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Eye.
4.

"The insulin receptor substrate (IRS-1) is a PEST protein that is susceptible to calpain degradation in vitro."
Smith L.K. , Bradshaw M. , Croall D.E. , Garner C.W.
Biochem. Biophys. Res. Commun.196:767-772(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: TURNOVER
5.

"Non-SH2 domains within insulin receptor substrate-1 and SHC mediate their phosphotyrosine-dependent interaction with the NPEY motif of the insulin-like growth factor I receptor."
Craparo A. , O'Neill T.J. , Gustafson T.A.
J. Biol. Chem.270:15639-15643(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH IGF1R
6.

"Distinct modes of interaction of SHC and insulin receptor substrate-1 with the insulin receptor NPEY region via non-SH2 domains."
He W. , O'Neill T.J. , Gustafson T.A.
J. Biol. Chem.270:23258-23262(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH INSR
7.

"Phosphotyrosine-dependent interaction of SHC and insulin receptor substrate 1 with the NPEY motif of the insulin receptor via a novel non-SH2 domain."
Gustafson T.A. , He W. , Craparo A. , Schaub C.D. , O'Neill T.J.
Mol. Cell. Biol.15:2500-2508(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH INSR
8.

"Adipose-specific expression, phosphorylation of Ser794 in insulin receptor substrate-1, and activation in diabetic animals of salt-inducible kinase-2."
Horike N. , Takemori H. , Katoh Y. , Doi J. , Min L. , Asano T. , Sun X.J. , Yamamoto H. , Kasayama S. , Muraoka M. , Nonaka Y. , Okamoto M.
J. Biol. Chem.278:18440-18447(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF SER-794;PHOSPHORYLATION AT SER-794
9.

"Protein kinase C Theta inhibits insulin signaling by phosphorylating IRS1 at Ser(1101)."
Li Y. , Soos T.J. , Li X. , Wu J. , Degennaro M. , Sun X. , Littman D.R. , Birnbaum M.J. , Polakiewicz R.D.
J. Biol. Chem.279:45304-45307(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-1101
10.

"ALK receptor tyrosine kinase promotes cell growth and neurite outgrowth."
Motegi A. , Fujimoto J. , Kotani M. , Sakuraba H. , Yamamoto T.
J. Cell Sci.117:3319-3329(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION BY ALK;INTERACTION WITH ALK
11.

"Deletion of SOCS7 leads to enhanced insulin action and enlarged islets of Langerhans."
Banks A.S. , Li J. , McKeag L. , Hribal M.L. , Kashiwada M. , Accili D. , Rothman P.B.
J. Clin. Invest.115:2462-2471(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH SOCS7
12.

"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Rush J. , Moritz A. , Lee K.A. , Guo A. , Goss V.L. , Spek E.J. , Zhang H. , Zha X.-M. , Polakiewicz R.D. , Comb M.J.
Nat. Biotechnol.23:94-101(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-662;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
13.

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V. , Blagoev B. , Gnad F. , Macek B. , Kumar C. , Mortensen P. , Mann M.
Cell127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-629 AND SER-636;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
14.

"Recruitment of insulin receptor substrate-1 and activation of NF-kappaB essential for midkine growth signaling through anaplastic lymphoma kinase."
Kuo A.H. , Stoica G.E. , Riegel A.T. , Wellstein A.
Oncogene26:859-869(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH ALK;FUNCTION
15.

"S6K directly phosphorylates IRS-1 on Ser-270 to promote insulin resistance in response to TNF-(alpha) signaling through IKK2."
Zhang J. , Gao Z. , Yin J. , Quon M.J. , Ye J.
J. Biol. Chem.283:35375-35382(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-270; SER-307; SER-636 AND SER-1101
16.

"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T. , Yi W. , Lu B. , Park S.K. , Xu T. , Lee J.-D. , Yates J.R. III
J. Proteome Res.7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
17.

"The CUL7 E3 ubiquitin ligase targets insulin receptor substrate 1 for ubiquitin-dependent degradation."
Xu X. , Sarikas A. , Dias-Santagata D.C. , Dolios G. , Lafontant P.J. , Tsai S.C. , Zhu W. , Nakajima H. , Nakajima H.O. , Field L.J. , Wang R. , Pan Z.Q.
Mol. Cell30:403-414(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: UBIQUITINATION;PHOSPHORYLATION AT SER-307; SER-312; SER-527 AND SER-636;MUTAGENESIS OF SER-307; SER-312; SER-527; SER-636 AND SER-639
18.

"Large-scale proteomics analysis of the human kinome."
Oppermann F.S. , Gnad F. , Olsen J.V. , Hornberger R. , Greff Z. , Keri G. , Mann M. , Daub H.
Mol. Cell. Proteomics8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
19.

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V. , Lundgren D.H. , Hwang S.-I. , Rezaul K. , Wu L. , Eng J.K. , Rodionov V. , Han D.K.
Sci. Signal.2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-348;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Leukemic T-cell.
20.

"The double-stranded RNA-dependent protein kinase differentially regulates insulin receptor substrates 1 and 2 in HepG2 cells."
Yang X. , Nath A. , Opperman M.J. , Chan C.
Mol. Biol. Cell21:3449-3458(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT SER-312 AND TYR-941
21.

"Structural basis for IL-4 receptor phosphopeptide recognition by the IRS-1 PTB domain."
Zhou M.-M. , Huang B. , Olejniczak E.T. , Meadows R.P. , Shuker S.B. , Miyazaki M. , Trueb T. , Shoelson S.E. , Fesik S.W.
Nat. Struct. Biol.3:388-393(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR OF 157-267
22.

"Crystal structure of the pleckstrin homology-phosphotyrosine binding (PH-PTB) targeting region of insulin receptor substrate 1."
Dhe-Paganon S. , Ottinger E.A. , Nolte R.T. , Eck M.J. , Shoelson S.E.
Proc. Natl. Acad. Sci. U.S.A.96:8378-8383(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 4-267
23.

"Structure and autoregulation of the insulin-like growth factor 1 receptor kinase."
Favelyukis S. , Till J.H. , Hubbard S.R. , Miller W.T.
Nat. Struct. Biol.8:1058-1063(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 889-902 IN COMPLEX WITH IGF1R
24.

"Aminoacid polymorphisms of insulin receptor substrate-1 in non-insulin-dependent diabetes mellitus."
Almind K. , Bjoerbaek C. , Vestergaard H. , Hansen T. , Echwald S. , Pedersen O.
Lancet342:828-832(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS PRO-512 AND ARG-971
25.

"Deletion of Gly723 in the insulin receptor substrate-1 of a patient with noninsulin-dependent diabetes mellitus."
Esposito D.L. , Mammarella S. , Ranieri A. , della Loggia F. , Capani F. , Consoli A. , Mariani-Costantini R. , Caramia F.G. , Cama A. , Battista P.
Hum. Mutat.7:364-366(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT NIDDM GLY-723 DEL
26.

"Novel allele of the insulin receptor substrate-1 bearing two non-conservative amino acid substitutions in a patient with noninsulin-dependent diabetes mellitus."
Mammarella S. , Creati B. , Esposito D.L. , Arcuri P. , della Loggia F. , Capani F. , Mariani-Costantini R. , Caramia F.G. , Battista P. , Cama A.
Hum. Mutat.11:411-411(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS NIDDM TYR-1043 AND TYR-1095
27.

"The Gly-->Arg(972) amino acid polymorphism in insulin receptor substrate-1 affects glucose metabolism in skeletal muscle cells."
Hribal M.L. , Federici M. , Porzio O. , Lauro D. , Borboni P. , Accili D. , Lauro R. , Sesti G.
J. Clin. Endocrinol. Metab.85:2004-2013(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION OF VARIANT ARG-971
28.

"The Arg(972) variant in insulin receptor substrate-1 is associated with an atherogenic profile in offspring of type 2 diabetic patients."
Marini M.A. , Frontoni S. , Mineo D. , Bracaglia D. , Cardellini M. , De Nicolais P. , Baroni A. , D'Alfonso R. , Perna M. , Lauro D. , Federici M. , Gambardella S. , Lauro R. , Sesti G.
J. Clin. Endocrinol. Metab.88:3368-3371(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ASSOCIATION OF VARIANT ARG-971 WITH NIDDM
29.

"Genetic polymorphism PC-1 K121Q and ethnic susceptibility to insulin resistance."
Abate N. , Carulli L. , Cabo-Chan A. Jr. , Chandalia M. , Snell P.G. , Grundy S.M.
J. Clin. Endocrinol. Metab.88:5927-5934(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT ARG-971
30.

"A novel T608R missense mutation in insulin receptor substrate-1 identified in a subject with type 2 diabetes impairs metabolic insulin signaling."
Esposito D.L. , Li Y. , Vanni C. , Mammarella S. , Veschi S. , Della Loggia F. , Mariani-Costantini R. , Battista P. , Quon M.J. , Cama A.
J. Clin. Endocrinol. Metab.88:1468-1475(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT ARG-608;CHARACTERIZATION OF VARIANT ARG-608
31.

"G972R IRS-1 variant impairs insulin regulation of endothelial nitric oxide synthase in cultured human endothelial cells."
Federici M. , Pandolfi A. , De Filippis E.A. , Pellegrini G. , Menghini R. , Lauro D. , Cardellini M. , Romano M. , Sesti G. , Lauro R. , Consoli A.
Circulation109:399-405(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT ARG-971;ASSOCIATION WITH ENDOTHELIAL DYSFUNCTION;CARDIOVASCULAR DISEASE
32.

"Human insulin receptor substrate-1 (IRS-1) polymorphism G972R causes IRS-1 to associate with the insulin receptor and inhibit receptor autophosphorylation."
McGettrick A.J. , Feener E.P. , Kahn C.R.
J. Biol. Chem.280:6441-6446(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT ARG-971;MOLECULAR MECHANISM OF LINKAGE TO NIDDM

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