ITIH3 ELISA Kit for Human(Homo sapiens) | EIAab.comITIH3 ELISA Kit for Human(Homo sapiens)

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Index > Protein center > ITIH3(Gene name) > Human

ITIH3 (Gene name),

Inter-alpha-trypsin inhibitor heavy chain H3 (Protein name ), ITIH3_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

ITIH3;

Protein name:

Inter-alpha-trypsin inhibitor heavy chain H3(ITI heavy chain H3;ITI-HC3;Inter-alpha-inhibitor heavy chain 3);

Alternative:

Serum-derived hyaluronan-associated protein(SHAP);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

I-alpha-I plasma protease inhibitors are assembled from one or two heavy chains (H1, H2 or H3) and one light chain, bikunin. Inter-alpha-inhibitor (I-alpha-I) is composed of H1, H2 and bikunin, inter-alpha-like inhibitor (I-alpha-LI) of H2 and bikunin, and pre-alpha-inhibitor (P-alpha-I) of H3 and bikunin.

Function:

May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

890

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

750:

800:

850:

890:

QKDYRKDASI | GTKVVCWFVH | NNGEGLIDGV | HTDYIVPNLF |

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Hs.76716

Pfam:

PF06668

MIM:

146650

SMR:

Q06033

STRING:

9606.ENSP00000415769

String:

Q06033

KEGG:

hsa:3699

Uniprot:

Q06033

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ELISA Kit for Human ITI heavy chain H3

Cat.:

E13559r

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Packing:

96T

ELISA Kit for Human ITI heavy chain H3

Cat.:

E13559h

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Manual:

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Packing:

96T

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ELISA Kit for Human ITI heavy chain H3

MSDS:

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ELISA Kit for Human ITI heavy chain H3

Cat.:

E13559b

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MSDS:

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Packing:

96T

ELISA Kit for Human ITI heavy chain H3

Cat.:

E13559m

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Manual:

Looking for manual.

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Packing:

96T

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CLIA Kit for Human ITI heavy chain H3

Cat.:

U13559m

Price:

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Manual:

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Packing:

96T

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CLIA Kit for Human ITI heavy chain H3

MSDS:

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CLIA Kit for Human ITI heavy chain H3

Cat.:

U13559r

Price:

Please sign in first.

MSDS:

Please sign in first.

Packing:

96T

CLIA Kit for Human ITI heavy chain H3

Cat.:

U13559b

Price:

Please sign in first.

MSDS:

Please sign in first.

Packing:

96T

CLIA Kit for Human ITI heavy chain H3

Cat.:

U13559h

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human ITI heavy chain H3

Cat.:

P13559Rb-r

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human ITI heavy chain H3

Cat.:

P13559Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human ITI heavy chain H3

Cat.:

P13559Rb-m

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human ITI heavy chain H3

Protein for Human ITI heavy chain H3

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.8%

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery was determined by spiking various levels of ITIH3 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	104	94-105
Plasma	102	93-106

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ITIH3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-113%	97-109%	95-111%	98-109%
Saliva(n=3)	94-108%	96-108%	97-107%	95-107%
heparin plasma(n=5)	93-110%	98-105%	99-106%	94-110%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
Saliva(n=3)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Human pre-alpha-trypsin inhibitor-precursor heavy chain. cDNA and deduced amino-acid sequence."
Bourguignon J. , Diarra-Mehrpour M. , Thiberville L. , Bost F. , Sesboue R. , Martin J.-P.
Eur. J. Biochem.212:771-776(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2);VARIANT LYS-315
tissue: Liver.
2.

"Human inter-alpha-trypsin inhibitor heavy chain H3 gene. Genomic organization, promoter analysis, and gene linkage."
Diarra-Mehrpour M. , Sarafan N. , Bourguignon J. , Bonnet F. , Bost F. , Martin J.-P.
J. Biol. Chem.273:26809-26819(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
tissue: Blood.
3.

Suzuki Y. , Sugano S. , Totoki Y. , Toyoda A. , Takeda T. , Sakaki Y. , Tanaka A. , Yokoyama S.
Submitted (2005-04) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue: Liver.
4.

"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M. , Scherer S.E. , Kaul R. , Wang J. , Yu J. , Sudbrak R. , Buhay C.J. , Chen R. , Cree A. , Ding Y. , Dugan-Rocha S. , Gill R. , Gunaratne P. , Harris R.A. , Hawes A.C. , Hernandez J. , Hodgson A.V. , Hume J. , more...
Nature440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
6.

"Tandem orientation of the inter-alpha-trypsin inhibitor heavy chain H1 and H3 genes."
Diarra-Mehrpour M. , Bourguignon J. , Sarafan N. , Bost F. , Sesbouee R. , Muschio-Bonnet F. , Martin J.-P.
Biochim. Biophys. Acta1219:551-554(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-27 (ISOFORM 2)
7.

"Analysis of inter-alpha-trypsin inhibitor and a novel trypsin inhibitor, pre-alpha-trypsin inhibitor, from human plasma. Polypeptide chain stoichiometry and assembly by glycan."
Enghild J.J. , Thoegersen I.B. , Pizzo S.V. , Salvesen G.
J. Biol. Chem.264:15975-15981(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 34-53; 467-481 AND 501-519
8.

"Human plasma inter-alpha-trypsin inhibitor is encoded by four genes on three chromosomes."
Diarra-Mehrpour M. , Bourguignon J. , Sesboue R. , Mattei M.-G. , Passage E. , Salier J.-P. , Martin J.-P.
Eur. J. Biochem.179:147-154(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 345-890 (ISOFORMS 1/2)
tissue: Liver.
9.

"Chondroitin 4-sulfate covalently cross-links the chains of the human blood protein pre-alpha-inhibitor."
Enghild J.J. , Salvesen G. , Hefta S.A. , Thoegersen I.B. , Rutherfurd S. , Pizzo S.V.
J. Biol. Chem.266:747-751(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 635-651;CROSS-LINK SITE TO BIKUNIN
10.

"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
Liu T. , Qian W.-J. , Gritsenko M.A. , Camp D.G. II , Monroe M.E. , Moore R.J. , Smith R.D.
J. Proteome Res.4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-91 AND ASN-580
tissue: Plasma.

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